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Umbilical cord mesenchymal stem cell injection liquid, preparation method and application thereof

A stem cell and injection technology, applied in the field of stem cells, can solve the problems of less application of acute rejection, painful economic burden for patients, low immunogenicity, etc., and achieve the effects of easy industrial preparation, long storage time, and reduced incidence.

Inactive Publication Date: 2016-12-21
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, except for re-liver transplantation, conventional treatment methods are often ineffective or even ineffective, and re-transplantation of liver brings a greater economic burden and mental and physical pain to patients
[0004] With the continuous development of stem cell biology research, MSC has biological characteristics such as low immunogenicity and strong immune regulation (can evade immune recognition, suppress immune response), inflammatory chemotaxis, tissue repair, etc. Injection of MSC can improve the immune tolerance after transplantation, prolong the survival time of the graft, and has also been effectively used in the treatment of graft-versus-host disease, especially in the treatment of immune rejection such as kidney transplantation and bone marrow transplantation. effect, but it is less used in the treatment of acute rejection after liver transplantation

Method used

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  • Umbilical cord mesenchymal stem cell injection liquid, preparation method and application thereof
  • Umbilical cord mesenchymal stem cell injection liquid, preparation method and application thereof
  • Umbilical cord mesenchymal stem cell injection liquid, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Preparation method of umbilical cord mesenchymal stem cells

[0069] Under sterile conditions, take out the fresh umbilical cord that has been away from the mother for no more than 2 hours, and screen whether the mother is carrying infectious disease pathogens (negative for hepatitis B surface antigen, hepatitis C antibody, syphilis antibody, and AIDS antibody), and place the fresh umbilical cord in a double Antibiotic PBS was transported back to the laboratory in a 4°C incubator.

[0070] Clean the fresh umbilical cord with sterile PBS, then disinfect it with 75% ethanol, and then clean it with PBS. Finally, cut the umbilical cord to 1~3cm 3 Evenly inoculate the pieces with the size of 0.5 ~ 1cm in the petri dish, slowly add 50μL ~ 100μL / cm 2 Complete medium, placed at 37°C, CO 2 The concentration was 5% in the incubator for static culture.

[0071] After the cells adhere to the wall and fuse, digest with trypsin, centrifuge, divide into multiple culture ...

Embodiment 2

[0084] Prepare 100mL stem cell injection: 30mL of human serum albumin stock solution with albumin concentration of 10% (final concentration of mass volume ratio is 3%), low molecular weight heparin calcium 0.1mL (volume percentage content is 0.1%), vitamin C 0.5 g (mass volume ratio final concentration is 0.5%), pearl ginsenoside 12.5g (mass volume ratio final concentration is 12.5%) and 0.9% sodium chloride injection 69.9mL are mixed, and the collected P5 generation umbilical cord mesenchymal stem cells (final cell concentration 1×10 6 individual / mL) in the mixed solution, resuspended in The cells are stored in the cryopreservation bag, and the preparation is complete.

[0085] The reserved samples of the umbilical cord mesenchymal stem cell injection prepared above are tested for endotoxin, bacteria and fungi, and mycoplasma. If the test results are all negative, it is qualified.

Embodiment 3

[0087] Prepare 100mL stem cell injection: 5mL of human serum albumin stock solution with albumin concentration of 20% (final concentration of mass volume ratio is 1%), low molecular weight heparin calcium 0.1mL (volume percentage is 0.1%), vitamin C 0.3 g (mass volume ratio final concentration is 0.3%), pearl ginsenoside 5g (mass volume ratio final concentration is 5%) and 0.9% sodium chloride injection 94.9mL are mixed, and the collected P5 generation umbilical cord mesenchymal stem cells ( Cell final concentration 1×10 6 individual / mL) in the mixed solution, resuspended in The cells are stored in the cryopreservation bag, and the preparation is complete.

[0088] The reserved samples of the umbilical cord mesenchymal stem cell injection prepared above are tested for endotoxin, bacteria and fungi, and mycoplasma. If the test results are all negative, it is qualified.

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Abstract

The invention relates to the technical field of stem cells and especially relates to an umbilical cord mesenchymal stem cell injection liquid, a preparation method and an application thereof. The injection liquid comprises umbilical cord mesenchymal stem cells, human albumin, low molecular heparin calcium, vitamin C, panax japonicus majoris saponin and a solvent. The injection liquid is prepared from the umbilical cord mesenchymal stem cells, so that the materials are convenient to obtain and the method does not violate ethical issue. The injection liquid can effectively prolong preservative time of the cells and the preserved stem cells have high activity, so that the injection liquid is convenient to use. After refusion, the injection can promote differentiation and maturation of the mesenchymal stem cell to liver cells, thus reducing incidence rate of GVHD.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to an umbilical cord mesenchymal stem cell injection and a preparation method and application thereof. Background technique [0002] The number of people infected with hepatitis B virus (HBV) in my country has reached 120 million, and the number of patients with chronic hepatitis is as high as more than 30 million. Among them, viral hepatitis B (hepatitis B) is the main cause of liver cirrhosis and primary hepatocellular carcinoma (liver cancer). The number of deaths due to liver cirrhosis, liver cancer, and liver failure is as high as 300,000 to 500,000, which seriously threatens people's health. For end-stage hepatitis, cirrhosis, and hereditary and metabolic liver diseases, orthotopic liver transplantation is currently the only sure and effective treatment, but there are various complications after liver transplantation, mainly including infection complications and rejection ,...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K9/08A61K35/51A61P37/06A61K31/727A61K31/375
CPCA61K9/0019A61K9/08A61K31/375A61K31/727A61K35/28A61K35/51A61K36/25A61K36/344A61K38/385A61K2300/00
Inventor 葛啸虎陈海佳王一飞李丽娟王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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