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Nano-fiber surface-modified xenogeneic acellular nerve graft and preparation method thereof

A surface modification, nanofiber technology, applied in fiber treatment, coating, medical science and other directions, can solve the problems of scar formation, affecting the surgical effect, unfavorable nerve connection, etc., to prevent tissue adhesion, firm bonding, and low price. Effect

Inactive Publication Date: 2017-06-30
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the above-mentioned acellular nerve graft is implanted in the body, although it can repair the nerve defect, at the same time, it will definitely promote the proliferation of fibroblasts, secrete a large amount of collagen fibers, and form scars and adhesions, which is not conducive to the connection between nerves and affects Surgical effects, sometimes leading to serious postoperative complications

Method used

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  • Nano-fiber surface-modified xenogeneic acellular nerve graft and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Dissolve polylactic acid-caprolactone copolymer with a weight average molecular weight of 200,000 in hexafluoroisopropanol at a concentration of 0.08g / ml. After stirring at room temperature to form a uniform and stable solution, place it in In the syringe, a high voltage power supply is connected to the needle of the syringe.

[0041] 2. The roller with the xenogeneic decellularized nerve graft fixed on it is used as the collecting device, and the rotating speed is 100 rpm. Start the electrospinning process, control the solution supply flow rate at 1ml / h, apply a voltage of 15KV, and set the distance between the high voltage terminal and the ground terminal at 15cm.

[0042] 3. Washing the collected nanofiber-modified acellular nerve graft with ethanol and deionized water for 12 hours respectively, and repeating 5 times.

[0043] 4. Freeze-dry the product in a freeze dryer (at a temperature of -30°C) for 10 hours, then sterilize it with ethylene oxide and package it...

Embodiment 2

[0046] 1. Dissolve racemic polylactic acid with a weight average molecular weight of 300,000 in dichloromethane at a concentration of 0.075g / ml. After stirring at room temperature to form a uniform and stable solution, put it into a syringe. Connect to high voltage power supply.

[0047] 2. Take the roller fixed with the heterogeneous decellularized nerve as the collection device, and the rotation speed is 250 rpm. Start the electrospinning process, control the solution supply flow rate at 3ml / h, apply a voltage of 12.5KV, and set the distance between the high voltage end and the ground end at 10cm.

[0048] 3. The collected nanofiber-modified acellular nerve graft was washed with ethanol and deionized water for 6 hours each, and repeated 3 times.

[0049] 4. Freeze-dry the product in a lyophilizer (at a temperature of -20°C) for 8 hours, and then sterilize and package it with cobalt-60 with an irradiation dose of 15KGy to obtain the final product. The schematic diagram is as...

Embodiment 3

[0052] 1. Dissolve polylactic acid-glycolic acid copolymer with a weight average molecular weight of 150,000 in acetone at a concentration of 0.15g / ml. After stirring at room temperature to form a uniform and stable solution, put it into a syringe, and the syringe needle Connect to high voltage power supply.

[0053] 2. Take the roller fixed with the xenogeneic decellularized nerve as the collection device, and the rotation speed is 300 rpm. Start the electrospinning process, control the solution supply flow rate at 2ml / h, apply a voltage of 10KV, and set the distance between the high voltage terminal and the ground terminal at 12.5cm.

[0054] 3. Washing the collected nanofiber-modified acellular nerve graft with ethanol and deionized water for 8 hours respectively, and repeating 4 times.

[0055] 4. Freeze-dry the product in a lyophilizer (at a temperature of -15°C) for 6 hours, then sterilize it with an irradiation dose of 13KGy electron beams and package it to obtain the ...

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Abstract

The invention discloses a nano-fiber surface-modified xenogeneic acellular nerve graft and a preparation method thereof. The nano-fiber surface-modified xenogeneic acellular nerve graft consists of an xenogeneic acellular nerve graft and a degradable polyester nano-fiber membrane adhered onto the surface of the xenogeneic acellular nerve graft, wherein the xenogeneic acellular nerve graft is an extracellular matrix obtained by performing acellular disposal on an isolated peripheral nerve of a healthy mammal. The nano-fiber surface-modified xenogeneic acellular nerve graft is extremely low in moisture content after freeze-drying treatment and stable in performance, and can realize no protection liquid and normal-temperature preservation after being sterilized and packaged. The nano-fiber surface-modified xenogeneic acellular nerve graft disclosed by the invention is wide in source, low in cost, free from immunogenicity, good in biocompatibility and safe to use; a microstructure of the peripheral nerve extracellular matrix is completely retained, and a natural pipeline bracket and a nutrition environment are provided for nerve axonal regeneration and nerve conduction function recovery.

Description

technical field [0001] The invention relates to a heterogeneous decellularized nerve graft with nanofiber surface modification and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Peripheral nerve injury may cause partial or total loss of body sensory and motor functions, which seriously affects the functioning of the body and the quality of life of the patient. The repair of peripheral nerve defects, especially the repair of long-distance defects, has always been a difficult clinical problem. At present, the most commonly used method is to use autologous nerve transplantation to bridge the stump to repair the injury. Although the repair effect is good, there are shortcomings such as limited sources, secondary trauma and easy formation of donor site neuromas, which greatly limit its clinical application. . Nerve conduit repair materials developed in recent years have certain effects in repairing peripheral nerve de...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/18A61L27/50A61L27/56D01D5/00
CPCA61L27/3633A61L27/18A61L27/3675A61L27/50A61L27/56A61L2430/32D01D5/003D01D5/0084C08L67/04
Inventor 窦源东尹晓彤孙先昌陈振华任志伟
Owner YANTAI ZHENGHAI BIO TECH
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