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A method for efficiently preparing mannose acid by multi-microbial step-by-step fermentation

A mannose acid, step-by-step fermentation technology, applied in the field of bioengineering and chemical engineering, can solve problems such as difficulties and achieve the effect of improving product yield and purity

Active Publication Date: 2020-10-30
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, based on existing technologies and techniques, it is relatively difficult to prepare high-purity mannose acid (salt) from raw materials such as konjac

Method used

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  • A method for efficiently preparing mannose acid by multi-microbial step-by-step fermentation

Examples

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Effect test

Embodiment example 1

[0022] In a 250ml Erlenmeyer flask system, add 50mL of 50g / L mannose and 30g / L glucose solution. First, insert Saccharomyces cerevisiae to a cell concentration of 2g / L (dry basis) and place it in a constant temperature reactor at 170rpm and 30°C for anaerobic ethanol fermentation. After 16 hours of reaction, the glucose concentration is lower than 3g / L and the remaining mannose is 44.5g / L to stop the ethanol fermentation. reaction. The centrifugation method was adopted, and the Saccharomyces cerevisiae was fully settled by centrifugation under the condition of 6000rpm for 10min. The isolated ethanol fermentation broth containing a large amount of mannose was inoculated into Gluconobacter oxydans to a cell concentration of 8 g / L (dry basis), and placed in a constant temperature reactor at 220 rpm and 30° C. for whole-cell catalysis. Add 10g / L calcium carbonate to maintain the pH value of the catalytic system at around 4.5-6.0. Stop the reaction until the mannose is completely...

Embodiment example 2

[0024] 1000g (dry basis) konjac is mechanically ground to powder, transferred to a 15L stainless steel cooking kettle, added 1.25% dilute hydrochloric acid, and the solid-liquid ratio (1:10, w / v) is fully impregnated. Heating to 120° C. with an electric jacket, reacting for 60 minutes, and filtering to obtain a dilute hydrochloric acid hydrolyzate, which contains 44.2 g / L mannose and 25.9 g / L glucose. The dilute acid hydrolyzate was neutralized to pH 6.5 by calcium hydroxide and then transferred to a 15L mechanically stirred reactor. Add Candida shohata to a cell concentration of 2g / L (dry basis). The reaction temperature was controlled at 28-30° C. and the mechanical stirring rate was 300 r / min, and the concentration of glucose and mannose in the hydrolyzate and the recovery rate of mannose were monitored by high-performance liquid chromatography. After 12 hours of reaction, the concentration of glucose in the acid hydrolyzate was lower than 2g / L, and the recovery rate of ma...

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Abstract

The invention discloses a method for efficiently preparing mannonic acid from multiple microorganisms through different steps of fermentation. The method is mainly characterized by comprising the following steps: in a konjak diluted acid hydrolysis system with 40-50g / L of mannose and 23-28g / L of glucose, selectively converting glucose in a raw material hydrolysis liquid into ethanol by using candida shehatae or brewer's yeast; when the concentration of the glucose is lower than 3g / L, terminating the reaction in time, separating a fermentation liquid from yeast cells, introducing gluconobacteroxydans into the separated fermentation liquid, and performing biological conversion, wherein the glucose fermentation product, namely the ethanol, is capable of effectively inhibiting later catabolism of the gluconobacter oxydans to a fermentation product, namely mannonic acid, of the ethanol, and furthermore the product yield of the mannonic acid is effectively increased. By adopting the method,the product ethanol is not removed in the glucose fermentation process, the later yield of the mannonic acid is greater than 74%, and the product mass volume concentration is greater than 3%.

Description

[0001] 1. Technical field [0002] The invention relates to the fields of bioengineering and chemical engineering, in particular to a method for efficiently preparing mannose acid by multi-microbe step-by-step fermentation. [0003] 2. Background technology [0004] Organic acids are the third fermented products after antibiotics and amino acids in the world, and the market space is huge. Among them, sugar acid can be produced by biotransformation of sugar components in plant raw materials and is widely used in food, medicine, feed and other industries, as sour agent, cement water reducer, mud dispersant, glass cleaning agent, metallurgical remover, etc. Rust agents, metal ion chelators, and bio-based platform compounds. Compared with traditional gluconic acid, mannonic acid (salt), as a new hexonic acid, has its own unique processing and application properties, and its use is being continuously explored and expanded. [0005] Mannonic acid can be produced by oxidation of man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12P7/58C12R1/865C12R1/72C12R1/01
CPCC12P7/06C12P7/065C12P7/58Y02E50/10
Inventor 徐勇周鑫刘鑫露勇强朱均均俞娟李鑫余世袁
Owner NANJING FORESTRY UNIV
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