Loop-mediated isothermal amplification primer group of plesimonas shigelloides, nucleic acid test strip kit and detection method

Abstract

The invention discloses a loop-mediated isothermal amplification primer group of plesimonas shigelloides, a nucleic acid test strip kit and a detection method. The detection method comprises the following steps: (1) treating a sample; (2) treating a primer; (3) performing loop-mediated isothermal amplification reaction; and (4) detecting a nucleic acid test strip. The primer group is as follows: apair of inner primers, a pair of outer primers and a pair of loop primers are designed according to the sequence of a hugA gene which is one of important genes of an encoding plesimonas shigelloidesferroheme iron ion utilizing system, and 8 independent areas on a target sequence can be identified specifically. The kit can detect the plesimonas shigelloides rapidly and sensitively, an expensive instrument is not needed, reaction can be completed within one hour only by one constant-temperature water bath pot, operation is simple, cost is low, the reaction result is easy to observe, the specificity is high, and the kit is very suitable for on-site detection of export quarantine, a primary laboratory and a farm and easy to popularize and apply extensively.

Description

technical field [0001] The present invention relates to the field of biotechnology. Background technique [0002] Plesiomonas shigelloides is a Gram-negative, aerobic or facultatively anaerobic, oxidase-positive, motile bacillus that has been associated with human pathogenic Vibrio and Aeromonas It belongs to Vibriaceae together, and later studies found that it is closer to P. shigella and Proteus at the molecular level. In the second edition of "Bergey's Handbook of Systematic Bacteriology", P. shigella Bacteria were assigned to the Enterobacteriaceae, Proximonas genus. [0003] P.shigella-like is an important enteroinflammatory pathogen in humans, which can cause symptoms such as acute gastroenteritis and diarrhea in humans, and can also cause sepsis, meningitis, septic arthritis, osteomyelitis, peritonitis, cellulitis and pneumonia Symptoms of extraintestinal infection. [0004] P.shigella-like bacteria are widely distributed in nature, especially in aquatic ecological...

AI Technical Summary

Problems solved by technology

The microbial culture method is time-consuming and the culture conditions are complicated; the interpretation of physiological and biochemical identification results depends on the subjective judgment of the operator, resulting in poor repeatability of the results and easy misjudgment; molecular detection such as PCR and real-time fluorescent PCR technology requires expensive equipment and technology High standard

Due to their respective defects, most of the conventional pathogen detection technologies are not suitable for on-site rapid detection at the grassroots level

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