Plasma metabolism micromolecular markers related to lung cancer early diagnosis and application thereof

A technology for metabolizing small molecules and early diagnosis, applied in material separation, analysis materials, measurement devices, etc., can solve the problems of high missed or misdiagnosed rate, low detection rate of markers, limited clinical application, etc., and achieve strong correlation between outcomes , Improve sensitivity and specificity, and achieve the effect of accurate judgment

Active Publication Date: 2018-08-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, imaging and sputum exfoliation cytology are still the main means of screening for early lung cancer, especially the pathological types of lung cancer are diverse, and clinical diagnosis mainly relies on imaging examinations: chest X-ray, CT (computed tomography, computerized tomography) ), PET-CT (Positron EmissionTomography/Computer Tomography, Positron Emission Tomography, Positron Emission Computed Tomography), but these examinations have certain

Method used

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  • Plasma metabolism micromolecular markers related to lung cancer early diagnosis and application thereof
  • Plasma metabolism micromolecular markers related to lung cancer early diagnosis and application thereof
  • Plasma metabolism micromolecular markers related to lung cancer early diagnosis and application thereof

Examples

Experimental program
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Effect test

experiment example 1

[0043] One. Objectives and methods

[0044] 1. Specimen collection and processing

[0045] Collect whole blood samples of 43 healthy volunteers and 43 preoperative lung cancer patients and place them in K 2 In the EDTA blood collection tube, centrifuge at 4°C and 8000rpm for 10 minutes, take the upper layer of plasma and EP tube, and quickly place it in a cold storage at -80°C for testing. Take 20μL from the plasma samples of healthy volunteers, mix them to prepare QC samples, and store them in the same way.

[0046] 2. Main reagents

[0047] Heptadecanoic acid (content> 99.9%), methoxyamine hydrochloride (MOX, content> 99.9%), N-methyl trimethylsilyl trifluoroacetamide (MSTFA, content> 99.9%) and pyridine were purchased from Sigma-Aldrich (St. Louis, MO, USA); formic acid, methanol and acetonitrile (HPLC reagents, Merck, Germany); ultrapure water (MILIQ ultrapure water from Milipore, USA) ).

[0048] 3. Detection and identification of plasma differential metabolites

[0049] 3.1. UPL...

Embodiment 2

[0088] Example 2: Preparation of the detection kit:

[0089] Based on the plasma metabolism small molecule markers provided by the present invention, a detection kit is prepared, which contains the following components:

[0090] Standards for plasma metabolism small molecular markers: including propionic acid, aspartic acid, glucopyranoside, citric acid, allose, talose, galactopyranos, pyruvate, tryptophan, leucylpro Acid, glycerol phosphorylcholine, oleoylcarnitine, lysophosphatidylcholine (18:3), lysophosphatidylcholine (18:2), lysophosphatidylcholine (18:1), lysophosphatidylcholine Choline (22:6), lysophosphatidylethanolamine (20:0), lysophosphatidylethanolamine (18:2), tyrosylvaline;

[0091] Solvent for extracting plasma metabolites: a 1:1 ratio of methanol and acetonitrile mixed solution, 80% methanol aqueous solution (for UPLC-IT-TOF / MS sample preparation); methanol, methoxyamine hydrochloride and N-methyltrimethyl Silicon-based trifluoroacetamide (for GC-MS sample preparati...

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Abstract

The invention discloses a group of plasma metabolism micromolecular markers related to lung cancer early diagnosis and an application thereof. The plasma metabolism micromolecular markers comprise oneor more of the following markers: propionic acid, aspartic acid, furan glucoside, citric acid, allose, talose, galactopyranose, pyruvic acid, tryptophan, leucyl proline, glyceryl phosphoryl choline,oleoyl carnitine, lysophosphatidyl choline (18:3), lysophosphatidyl choline (18:2), lysophosphatidyl choline (18:1), lysophosphatidyl choline (22:6), lysophosphatidyl ethanolamine (20:0), lysophosphatidyl ethanolamine (18:2), and complex aminoacyl valine. Compared with the traditional protein biomarkers, the group of the provided markers has the stronger outcome relevance with diseases, and is stable, minimally invasive, and easy to detect, capable of greatly improving the sensitivity and the specificity of the related disease diagnosis, and suitable for the early clinical diagnosis and monitoring of lung cancer.

Description

Technical field [0001] The invention belongs to the technical field of biochemistry, and specifically relates to a group of small molecular markers of plasma metabolism related to the early diagnosis of lung cancer and their applications. Background technique [0002] Lung cancer is one of the tumors with increasing morbidity and mortality in the past half century, and it is the most harmful malignant tumor to human health and life. The 5-year survival rate of lung cancer has not improved significantly in the past 20 years, about 14%. Because most patients with lung cancer are already at an advanced stage when they are discovered, they can only receive palliative treatment, resulting in a short survival time. Therefore, early diagnosis and early treatment will significantly improve the survival of patients and increase the survival rate of lung cancer patients. At present, imaging and sputum exfoliation cytology are still the main methods for screening early lung cancer, especi...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822
Inventor 黄寅张尊建许风国惠慧华云飞杨旭萍李琳睿
Owner CHINA PHARM UNIV
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