Pig feed additive for improving immunity and pig feed with same

A pig feed additive and immunity technology, which is applied in the field of pig feed additives and pig feed, can solve the problems of irregular occurrence of stress-response diseases, failure to meet the requirements of pig breeding industry, increase production costs, etc., and achieve optimal intestinal flora Set up, improve the immunity of suckling pigs, and promote the effect of growth and reproduction

Inactive Publication Date: 2019-05-31
刘坚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It not only adds extra burden to the production process, but also increases more production costs
In addition, the respiratory tract, digestive tract, reproductive tract, mammary gland infection caused by many conditional pathogens and stress r...

Method used

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  • Pig feed additive for improving immunity and pig feed with same
  • Pig feed additive for improving immunity and pig feed with same
  • Pig feed additive for improving immunity and pig feed with same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The antibacterial effect of embodiment 1 quinoa bran

[0028] 1. Experimental materials and instruments

[0029] Experimental materials: quinoa bran, absolute ethanol, methanol, n-butanol, saline, diatomaceous earth, common broth medium, agar, Staphylococcus aureus, Escherichia coli, Salmonella, paper discs with a diameter of 5 mm, 96 orifice plate.

[0030] Experimental equipment: YXQ-LS-70A vertical pressure steam sterilizer, Shanghai Boxun Industrial Co., Ltd.; BPMJ-150F mold incubator, Shanghai Yiheng Scientific Instrument Co., Ltd.

[0031] 2. Solution preparation

[0032] N-butanol layer: Take 8.3 mg of the n-butanol layer and dissolve it in 2 mL of methanol to a concentration of 4.15 mg / mL.

[0033] Culture medium: take ordinary broth liquid medium, dissolve it in ultrapure water, and keep stirring, put it into a Erlenmeyer flask with a cotton stopper, autoclave at 121°C for 15 minutes, and set aside.

[0034] Bacterial liquid: Activate Staphylococcus aureus,...

Embodiment 2

[0051] The fermentation of embodiment 2 cassava residues

[0052] 1. Preparation of experimental materials

[0053] Pleurotus os-treatus and Coriolus versicolor were stored on the slope of the refrigerator at 4°C.

[0054] Activation medium: Potato liquid medium (PDY) (m / v): 20% potato extract, 2%-3% sucrose, sterilized at 121° C. for 30 minutes.

[0055] Solid fermentation medium: Weigh 50 g of fresh cassava residue (15 g in dry weight) into a 250 mL Erlenmeyer flask, seal with plastic film, and sterilize at 121°C for 30 minutes.

[0056] 2. Fermentation treatment of cassava residue

[0057] Divide small inoculated pieces from the potato dextrose agar (PDA) slant to PDY medium, culture on a shaker (28°C, 120r / min) for 7 days, add Pleurotus pellagra liquid and variegated cloud to the solid fermentation medium 5mL each of the mushroom solution was cultured statically at 28°C for 15 days.

[0058] 3. Test method

[0059] The content of lignin in cassava residues is based on t...

Embodiment 3

[0064] Antagonism experiment of embodiment 3 compound probiotic bacterial classification

[0065] Using the plate confrontation method, take the activated strains on the plate, inoculate Lactobacillus acidophilus, Bacillus subtilis and Saccharomyces boulardii in two pairs on the ordinary nutrient agar medium, and inoculate them in a constant temperature and humidity incubator at 40°C. Cultivate in medium for 3 days, observe whether the intersection is inhibited and the phenomenon of colony shrinkage and disappearance occurs, so as to judge whether there is antagonism among the strains. The results are shown in Table 5, there is no antagonistic effect between the strains, and they can grow well in the same environment, and can be used to prepare mixed bacterial agents.

[0066] Table 5 Antagonistic experiment results of three kinds of probiotics at 40°C

[0067] ("+" means antagonistic, "-" means no antagonistic)

[0068] bacteria

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Abstract

The invention discloses a pig feed additive for improving immunity and a pig feed with the same. The pig feed additive is prepared from, by weight, 2-20 parts of a rhizoma imperatae extraction solution, 0.3-1 part of folic acid, 0.05-0.3 part of vitamin B12 and 1-3 parts of a probiotics compound preparation; the probiotics compound preparation is prepared from 2-5*10<9> CFU/g lactic acid bacteria,0.8-1.5*10<8> CFU/g saccharomyces cerevisiae and 2-5*10<9> CFU/g bacillus subtilis. The pig feed additive has the advantages that nutrients required for the growth of live pigs are reasonably configured according to the growth characteristics of the live pigs, and the production performance and immunity of the live pigs can be effectively improved.

Description

technical field [0001] The invention belongs to the field of feed for livestock breeding, and in particular relates to a pig feed additive for improving immunity and a pig feed comprising the same. Background technique [0002] In recent years, pig diseases have occurred frequently. At present, in pig farms, the means of immunization and vaccination are generally used to improve the immunity of pigs. However, when the old disease is not gone and the new disease is coming again, a pig needs to be vaccinated several times or even more in a short life. It not only adds extra burden to the production process, but also increases more production costs. In addition, the respiratory tract, digestive tract, reproductive tract, mammary gland infection caused by many conditional pathogens and stress response diseases caused by non-conditional pathogen factors occur irregularly, so the specific immunity of pigs can only be improved by immunization and vaccination It has been unable to...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/14A23K10/18A23K10/30A23K10/37A23K10/38A23K20/147A23K20/174A23K50/30
CPCY02P60/87
Inventor 刘坚
Owner 刘坚
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