A ha-targeted double metal hydroxide-ultrasmall iron nanomaterial and its preparation and application
A technology of hydroxide and nanomaterials, applied in preparations for in vivo experiments, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of not finding doxorubicin and ultra-small iron nano Particle research reports and other issues, to achieve good blood circulation time in the body, broad prospects, and enhance the effect of nuclear magnetic resonance imaging
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Embodiment 1
[0080] Materials Synthesis:
[0081] (1) under stirring FeCl 3 (1081mg, Adamas reagent, 500g, Batch: P1276861) was dissolved in 40mL of diethylene glycol to form a homogeneous solution. Sodium citrate (471 mg, Sinopharm, 500 g of, batch number: 20140611) was added to the solution, and the mixture was heated to 80 deg.] C in a water bath until a clear solution is formed. Subsequently, sodium acetate (1312mg, Shanghai Chemical Reagent Ling, 500 g of, batch number: 20140124) was added to the mixture and dissolved, and then the mixture was transferred to a volume of 100mL Teflon-lined stainless steel autoclave and sealed in air . The autoclave was placed in an oven at 200 ℃ 4h. After cooling to RT, the black solution was collected by centrifugation (10000rpm, 5min) and 3 times to remove excess reagents and by-product was purified with ethanol. The resulting black product was redispersed in water and lyophilized to obtain a particle size of 3.2nm Fe 3 O 4 NPs powder for further use.
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Embodiment 2
[0086] To assess LDH-Fe 3 O 4 As -HA imaging MRI contrast agents, the material to pure Fe 3 O 4 Nanoparticles r 1 Relaxivity compared, r 1 Longitudinal relaxation time relaxation rate per unit molar concentration of iron, T may be of different concentrations 1 Fitting reciprocal relaxation time is calculated. Preparation Example 1 Fe measured by ICP-AES method embodiment of the test 3 O 4 , LDH-Fe 3 O 4 And LDH-Fe 3 O 4 -HA content of Fe element. We were prepared and the Fe concentration of 1.6mM 0.1,0.2,0.4,0.8 of LDH-Fe 3 O 4 500μL -HA aqueous solution by magnetic resonance imaging analyzer material T at different concentrations of Fe 1 Relaxation effects (such as Figure 8 ). After calculated Fe 3 O 4 , LDH-Fe 3 O 4 And LDH-Fe 3 O 4 -HA of r 1 Values were 0.42,5.53 and 4.38mM -1 s -1 . LDH-Fe 3 O 4 -HA of r 1 Higher than Fe 3 O 4 The r 1 value. LDH-Fe described in Example 1 was prepared 3 O 4 -HA NPs can be used as molecular MR imaging diagnosis excellent T 1 Positive contrast...
Embodiment 3
[0088] In B16 cells as a model, using the CCK-8 assay LDH-Fe 3 O 4 -HA NPs cytotoxicity and LDH-Fe 3 O 4 -HA / Antitumor effect of DOX NPs. Iron concentration of formulated 1000μg / mL LDH-Fe 3 O 4 -HA NPs mother liquor in PBS, and then formulated in the clean bench DOX concentration with sterile PBS and add 10μL of different concentrations of LDH-Fe 3 O 4-HA / PBS solution of DOX. Such that the final concentration of DOX 1.6,3.2,6.3,12.5 and 25μg / mL, the highest concentration corresponding to the content of the carrier to make a set. Overnight and sterilized with ultraviolet irradiation. The cell culture plate was placed in a 5% CO 2 , 37 ℃ continued for 24h and 48h. Then the culture was drained, washed two times with PBS, each well was then added 100μL fresh culture medium (containing 10μL CCK-8,90μL medium), cultured for 4h, the absorbance measured at 450nm using a microplate reader, and cell viability is calculated based on this value.
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