Kit for detecting antidepressant drug in serum through ultra-high performance liquid chromatography-tandem mass spectrometry technology

Abstract

The invention relates to a kit for detecting an antidepressant drug in serum through an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The kit is simple in pretreatment process, low in cost, high in sensitivity and high in specificity, separation and detection of the antidepressant drug are completed within 4.5 min, the accuracy and precision basically meet the requirements, the method can be used for quantitative analysis of the clinical antidepressant drug, and a reliable detection method is provided for monitoring the treatment concentration of the clinical antidepressant drug.

Description

technical field [0001] The invention belongs to the technical field of serum detection, and in particular relates to a kit for detecting antidepressant drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] At present, the commonly used drugs for the treatment of depression are divided according to their mechanism of action: norepinephrine reuptake inhibitors, serotonin (5-HT) reuptake inhibitors (SSRIs), 5-HT and norepinephrine reuptake inhibitors SNRIs, norepinephrine and specific serotonergic antidepressants (NaSSAs), 5-HT2 receptor antagonists and 5-HT reuptake inhibitors, norepinephrine and dopamine reuptake inhibitors and melatonin receptor agonists. [0003] The methods for measuring antidepressants in human serum or plasma mainly include HPLC-UV method, GC-MS method and LC-MS / MS method in domestic and foreign literature reports, such as the document "Quantification of 33 antidepressants by LC-MS / MS—comp...

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Problems solved by technology

[0003] The methods for measuring antidepressants in human serum or plasma mainly include HPLC-UV method, GC-MS method and LC-MS / MS method in domestic and foreign literature reports, such as the document "Quantification of 33 antidepressants by LC-MS / MS—comparative "validation in whole blood, plasma and serum" reported a liquid-liquid extraction method for the detection of 33 antidepressants, but the pretreatment required complex procedures such as liquid-liquid extraction, nitrogen blowing and concentration, and each sample collection took 30 minutes

For example, Chinese patent (CN 108982714 A) discloses "a simple and efficient method for detecting the concentration of venlafaxine and its active metabolite o-desvenlafaxine in human plasma". Carry out liquid-liquid extraction, then reconstitute the sample after nitrogen blowing, and then detect the concentration of the analyte by high performance liquid phase fluorescence method; the pretreatment of this invention is time-consuming and laborious, the amount of sample is large, and the analysis time of a single sample is longer

In addition, Chinese patent (CN 109655568 A) discloses a "method and kit for simultaneous determination of 35 psychotropic drugs by HPLC-MS". Although the pre-treatment is simple, the external standard method is used for quantification, and the accuracy is poor

For another example, Northwest Pharmaceutical Journal, Volume 34, Issue 6, November 2019, is entitled "Simultaneous Determination of the Mass Concentrations of Fluoxetine, Norfluoxetine, Paroxetine and Fluvoxamine in Human Plasma by LC-MS / MS Method" ", the invention uses 200 μL plasma for protein precipitation, and the sample volume is large; using roxithromycin as the internal standard of all analytes lacks universal applicability and accuracy, and there may be matrix effect interference; the linear range of the four drugs All the same, not clinically proven, lack of rationality

In addition, because clinical samples are extremely precious, the pre-treatment sample volume should be as small as possible, and the injection volume will seriously pollute the instrument and increase the maintenance cost of the instrument.

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