Application of microalgae carbon sequestration enhancer and culture medium containing enhancer

A culture medium and enhancer technology, applied in the direction of microorganism-based methods, microorganisms, single-cell algae, etc., can solve the problems of limited physical and chemical properties of culture fluid, low concentration of total inorganic carbon, and growth limitation of microalgae, and achieve no Effects of poisoning, improving carbon fixation efficiency, and improving conversion efficiency

Pending Publication Date: 2020-11-13
HENAN UNIVERSITY
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  • Abstract
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Problems solved by technology

However, limited by the physical and chemical properties of the culture medium, CO 2 The concentration of total inorganic carbon dissolved in the liquid phase is still not high, especially in the near-neutral pH environment, in the culture solution flowing out from the carbon supplementation site, part of the carbon source is lost to the air, and part of the carbon source is converted into carbonate form, thus causing carbon source limitation for microalgae growth

Method used

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  • Application of microalgae carbon sequestration enhancer and culture medium containing enhancer
  • Application of microalgae carbon sequestration enhancer and culture medium containing enhancer
  • Application of microalgae carbon sequestration enhancer and culture medium containing enhancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A columnar glass photoreactor (height 50cm, inner diameter 6cm) is used indoors for microalgae cultivation, and aeration stones (aperture 30-60μm) commonly used in this field are used for ventilation, and the light intensity provided by 8 fluorescent tubes is 100μmol / m 2 / s of 12h:12h (light:dark) lighting.

[0028] The cultured algae is Chlorella vulgaris, which comes from the freshwater algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences, numbered FACHB-8. In the control group, BG11 was used as medium to culture Chlorella, and the culture volume was 1L. 0.5% methanol by volume was added to the medium of the methanol experimental group; polyethylene glycol dimethyl ether was added to the medium of the NHD experimental group, with a final concentration of 5 mmol / L; propylene carbonate was added to the medium of the PC experimental group, and finally The concentration was 5mmol / L; N-methylpyrrolidone was added to the culture medium of the NMP experi...

Embodiment 2

[0037] A columnar glass photobioreactor was used to cultivate marine Nannochloropsis indoors. The algae species came from the Freshwater Algae Species Bank of the Institute of Hydrobiology, Chinese Academy of Sciences, number 926. The control group was cultured in f / 2 medium, and the experimental group was cultured in f / 2 medium supplemented with PC. The final concentration of PC was 5 mmol / L. Others are the same as in Example 1, the inoculation concentration of microalgae cells is 0.05g / L.

[0038] During the culture period, the average total inorganic carbon source concentration in the culture solution of the control group was 4.95mmol / L. The average total inorganic carbon source concentration in the culture medium of the methanol experimental group was 6.46mmol / L, which was 62.2% higher than that of the control group. The average biological carbon fixation efficiency of the control group was 121.2 mg / L / d after 8 days of batch culture, and the average biological carbon fixa...

Embodiment 3

[0040] Carry out an open track pool culture experiment. The runway pool has a circumference of 70 meters and a width of 3 meters. The algal species is Scenedesmus dimorphus, which comes from the freshwater algae species bank of the Institute of Hydrobiology, Chinese Academy of Sciences, number 496. Cultivate Scenedesmus dimorphus with BG11 medium, the depth of the culture medium is 20 cm; add PC to the final concentration of 5 mmol / L when preparing the medium; rely on the natural evaporation of water to maintain the temperature of the culture medium not exceeding 30°C; the flow of the culture medium Driven by the stirring impeller, the carbon replenishment adopts automatic control (there are related control devices and technology published documents, such as patents CN200410009360.4 and CN201210138598.1). The raceway pond culture in the medium without adding PC was used as the control group. The results showed that the average total inorganic carbon concentration in the cult...

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Abstract

The invention relates to application of a microalgae carbon sequestration enhancer in improving microalgae carbon sequestration efficiency. The invention also relates to a culture medium capable of improving the carbon sequestration efficiency of microalgae, the culture medium comprises a microalgae carbon sequestration enhancer, and the microalgae carbon sequestration enhancer is one or a combination of more of methanol, polyethylene glycol dimethyl ether, propylene carbonate or N-methyl pyrrolidone. The invention also relates to a method for culturing microalgae by using the culture medium to improve the carbon sequestration efficiency of microalgae. According to the microalgae carbon sequestration enhancer and the culture medium containing the microalgae carbon sequestration enhancer provided by the invention, the carbon sequestration efficiency of microalgae can be improved, and the microalgae carbon sequestration enhancer and the culture medium containing the microalgae carbon sequestration enhancer have no toxic effect on the microalgae and cannot be absorbed and consumed by the microalgae. By using the culture medium and the method provided by the invention, the cultured algae cells have high carbon sequestration efficiency, high carbonic anhydrase activity and high photosynthetic oxygen release rate, and are beneficial to treat CO2-containing tail gas by using microalgae, thereby achieving the effect of emission reduction.

Description

technical field [0001] The present invention relates to the field of environmental protection and microalgae cultivation, more particularly, relates to the application of microalgae carbon fixation enhancer in improving microalgae carbon fixation efficiency, the medium that can improve microalgae carbon fixation efficiency, and the use of the above medium for cultivation Microalgae to improve the efficiency of microalgae carbon fixation Background technique [0002] Microalgae Biological Method for CO Emission Reduction 2 It is of great significance to alleviate the greenhouse effect and develop a low-carbon economy. However, the microalgae carbon sequestration industry is still in its infancy, and there are some technical bottlenecks, such as low-concentration CO such as flue gas. 2 The low solubility of gases in water and the slow rate of bioconversion of carbon sources by microalgae need to be addressed. [0003] At present, the cultivation of microalgae is mostly carr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38B01D53/84B01D53/62C12R1/89
CPCC12N1/12C12N1/38B01D53/84B01D53/62B01D2257/504Y02A50/20Y02P20/59
Inventor 孙中亮王强周创创陈辉
Owner HENAN UNIVERSITY
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