Selenium oligosaccharide freeze-dried powder and application thereof to preparation of selenium-enriched feed for selenium-enriched culture of sea cucumbers
A technology of freeze-dried powder and selenium oligosaccharide, which is applied in the application, animal feed, glycosylase and other directions, can solve the problems of high viscosity, poor water solubility and large molecular weight of selenium polysaccharide, and achieves low molecular weight, easy absorption and low viscosity. Effect
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Embodiment 1
[0039] Example 1: Screening and Identification of Bacillus N1-1
[0040] 1. Screening and activation of Bacillus N1-1
[0041] Weigh 1g of deep-sea cold spring mud sediment, add 9mL of sterile seawater, take 0.1mL and gradually dilute to a concentration of 10-2~10-6, and spread it on the plate medium (5g peptone, 1g yeast powder, 0.1g ferric phosphate, 15g of agar powder, 1000mL of seawater filtered through a 0.22μm membrane, pH 7.6), cultured in a constant temperature incubator at 25°C. Pick a single colony according to the characteristics of the colony shape and size, and separate and purify it by streaking three times until a variety of pure bacterial cultures are obtained. Then, configure 2216E fermentation medium (5g peptone, 1g yeast powder, 0.1g iron phosphate, 1000mL seawater filtered by 0.22μm membrane, pH 7.6), return to normal temperature after high temperature sterilization, and inoculate the purified strain with an inoculation loop. Into the fermentation medium,...
Embodiment 2
[0046] Embodiment 2: the preparation of selenium oligosaccharide freeze-dried powder
[0047] (1) Bacillus N1-1 was inoculated into the fermentation medium with an inoculation amount of 2.5%, cultivated at 35°C and 150r / min at a constant temperature overnight, and the fermentation liquid was collected as selenide carrageenase enzyme liquid, and then the enzyme liquid A vacuum freeze-drying machine was used to carry out vacuum freeze-drying (conditions: pre-freezing temperature -40° C., protective agent trehalose concentration 2.0%, enzyme solution thickness 5 mm, and pre-freezing time 10 h) to obtain a selenized carrageenan-solidified enzyme preparation.
[0048] The activity of selenide carrageenase was determined by DNS (3,5-dinitrosalicylic acid) method. Specifically, 1 mL of enzyme solution was mixed with 1 mL of 0.2% selenide carrageenan substrate (pH = 7.4), and placed in a constant temperature water bath for 3.0 h at a temperature of 40 ° C. control group. Take 1mL of...
Embodiment 3
[0054] Embodiment 3: the cultivation of selenium-enriched sea cucumber
[0055] 1. Preparation of selenium oligosaccharide feed
[0056] Add the lyophilized selenium oligosaccharide powder prepared in Example 2 to the basal feed; specifically, mix all the components of the basal feed with the lyophilized selenium oligosaccharide powder, add water to a semi-dispersed state and mix well, then Use a granulator to process and granulate it, spray grease on the surface of the feed granules after granulation, and pack it after natural cooling, which is the selenium-enriched feed for selenium-enriched sea cucumber cultivation.
[0057] Wherein, the basic feed consists of the following components by mass: 3 parts of sargassum, 1 part of sargassum, 1.5 parts of large leafy vegetables, 1 part of kelp powder, 1 part of scallop powder, 1 part of shrimp powder, 1 part of oyster powder, 1 part of Haihong powder, 10 parts of sea mud, 0.4 part of oligosaccharide, 0.4 part of spore M3, and 0.3...
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