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Culture method of strain for sewage treatment and culture medium of culture method

A sewage treatment and culture medium technology, applied in the field of microbial cultivation, can solve the problems of high cost and complicated sewage treatment process, and achieve the effect of reducing usage, reducing cost and increasing the number of viable bacteria

Active Publication Date: 2021-07-23
吉安市傲农现代农业科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most enterprises carry out biological denitrification of sewage mainly through the purchase of ready-made composite bacterial powder for sewage treatment, and because the process of biological denitrification involves a variety of bacterial species, and the optimal growth conditions for different bacterial species are inconsistent, resulting in the need to The more complex sewage treatment process and related equipment and sites, such as the use of multi-stage treatment pools, need to monitor dissolved oxygen, pH, carbon sources, etc. at any time. Therefore, some enterprises have adopted a large increase in the amount of bacterial powder added to achieve the purpose of ammonia nitrogen removal in sewage. high cost

Method used

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  • Culture method of strain for sewage treatment and culture medium of culture method
  • Culture method of strain for sewage treatment and culture medium of culture method
  • Culture method of strain for sewage treatment and culture medium of culture method

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Experimental program
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Embodiment 1

[0089] The present embodiment provides a kind of cultivation method of bacterial classification for sewage treatment, and it comprises the following steps:

[0090] A. First-level training

[0091] Add water with 45% of the volume of the fermenter in the primary fermenter (300L), add the culture medium material (8kg) and stir evenly, then add water to make the volume 65% of the volume of the fermenter, sterilize at 121°C for 20 minutes, and sterilize Finally, the initial pH is adjusted to be 8, the temperature is 22°C, and the stirring speed is 50r / min, and the composite bacterial powder (Gandu composite strain GANDEW-MIX) of 2.5% of the medium weight is added to the medium for sewage treatment, and fermented for 24h. During the fermentation process, alkali supplementation (10wt% sodium hydroxide aqueous solution) is used to control the pH during the fermentation process to be not lower than 6.8 and not higher than 8.0, and the ventilation, stirring speed and temperature are a...

Embodiment 2

[0102] The difference between this embodiment and embodiment 1 is:

[0103] Each liter of medium contains 6g glucose, 2g yeast extract powder, 1.6g peptone, 1.6g urea, 1g ammonium sulfate, 0.1g sodium nitrate, 10g sodium bicarbonate, 1g sodium thiosulfate, 0.06g dipotassium hydrogen phosphate, 2g Magnesium sulfate, 0.01g manganese sulfate, 0.4g calcium chloride, 0.01g zinc chloride, 0.01g cobalt chloride, 0.02g ferrous sulfate, 0.01g ammonium molybdate, 0.2g cysteine ​​and 0.2g methionine acid, and the balance is water.

Embodiment 3

[0105] The difference between this embodiment and embodiment 1 is:

[0106] Each liter medium contains 8g glucose, 1.6g yeast extract powder, 2g peptone, 1g urea, 1.6g ammonium sulfate, 0.16g sodium nitrate, 8g sodium bicarbonate, 1.6g sodium thiosulfate, 0.1g dipotassium hydrogen phosphate, 1g magnesium sulfate, 0.006g manganese sulfate, 0.2g calcium chloride, 0.02g zinc chloride, 0.02g cobalt chloride, 0.01g ferrous sulfate, 0.02g ammonium molybdate, 0.1g cysteine ​​and 0.1g methyl sulfide acid, and the balance is water.

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Abstract

The invention discloses a culture method of a strain for sewage treatment and a culture medium of the culture method, and belongs to the technical field of microbial culture. Every 100 parts of preparation raw materials of the culture medium comprise the following components in parts by weight: 0.6-0.8 part of an organic carbon source, 0.44-0.6 part of an organic nitrogen source, 0.8-1 part of an inorganic carbon source, 0.11-0.116 part of an inorganic nitrogen source, 0.1246-0.249 part of a metal ion source, 0.106-0.17 part of a potential regulator and the balance water. The culture medium is suitable for culture of strains for sewage treatment, the viable count of the cultured strains can be effectively increased, a good sewage treatment effect can be obtained under the condition that the usage amount of bacterial powder is greatly reduced during sewage treatment, and the sewage treatment cost is reduced.

Description

technical field [0001] The invention relates to the technical field of microbial cultivation, in particular to a method for cultivating bacteria for sewage treatment and a culture medium thereof. Background technique [0002] With the rapid development of industry and the improvement of people's living standards, a large number of nitrogen-containing substances have entered the water body, seriously polluting the water body environment, and the nitrogen content in the water has a tendency to gradually increase. In terms of water body pollutant control, ammonia nitrogen has become the focus of my country's sewage treatment work. important content. [0003] Sewage treatment methods are mainly divided into physical and chemical denitrification and biological denitrification. Due to the disadvantages of using physical and chemical methods to treat ammonia nitrogen sewage, it is easy to cause secondary pollution to the environment and the treatment cost is high. At present, biolog...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12R1/38C12R1/01C12R1/07
CPCC12N1/38C12N1/20Y02W10/10
Inventor 张志榕龙毅王小芬郑甜力郭泗虎吴有林
Owner 吉安市傲农现代农业科技有限公司