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Culture medium suitable for seawater phage fermentation as well as preparation method and application of culture medium

A culture medium and phage technology, applied in the field of microorganisms, can solve the problems of high-efficiency and low-cost medium, undisclosed, easy precipitation of liquid medium, etc., achieve good culture effect, avoid precipitation, and reduce the effect of medium components

Active Publication Date: 2021-08-03
QINGDAO PHAGEPHARM BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, 2216E liquid culture medium is usually used as a culture medium for seawater-related bacteria or phages, but there is no targeted and highly effective method for seawater-related bacteriophages. cheap culture medium
At the same time, the 2216E liquid medium has the problem of easy precipitation

Method used

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  • Culture medium suitable for seawater phage fermentation as well as preparation method and application of culture medium
  • Culture medium suitable for seawater phage fermentation as well as preparation method and application of culture medium
  • Culture medium suitable for seawater phage fermentation as well as preparation method and application of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Mix peptone 5.0g, yeast extract powder 1.0g, ferric citrate 0.1g, sodium chloride 19.45g, magnesium chloride 5.98g, sodium sulfate 3.24g, calcium chloride 0.3g, potassium chloride 0.55g, sodium carbonate 0.16g , potassium bromide 0.08g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, disodium hydrogen phosphate 0.008g, water 1000ml, adjust the pH to 7.60, and sterilize at 121°C for 20 minutes.

[0035] 2) Inoculate three strains of Vibrio parahaemolyticus G107, DYW34-1, G36 and corresponding Vibrio parahaemolyticus phage PG31, PG124, PG32 respectively in the above-mentioned medium of 100Ml and the finished product 2216E liquid medium of 100ml commercially available, Fermentation was carried out at 37° C. and 170 rpm for 5 hours.

[0036] 3) The titer of the fermented broth was determined by the double-layer plate method, and the titer was obviously improved.

[0037]

[0038] It can be seen from the experimental results that the phage titer ob...

Embodiment 2

[0040] Prepare medium 350L of the present invention in a 500L fermentation tank, peptone 1750.0g, yeast extract powder 350.0g, ferric citrate 35.0g, sodium chloride 6807.5g, magnesium chloride 2093g, sodium sulfate 1134g, calcium chloride 105g, potassium chloride 192.5 g, sodium carbonate 56g, potassium bromide 28g, boric acid 7.7g, sodium silicate 1.4g, sodium fluoride 0.84g, disodium hydrogen phosphate 2.8g, water 35L, adjust the pH to 7.60, and sterilize at 121°C for 20 minutes.

[0041] Vibrio parahaemolyticus DYW34-1 and the corresponding Vibrio parahaemolyticus phage PG124 were inoculated into a fermenter according to a certain ratio for expanded culture, and fermented at 37° C. and 170 rpm for 5 hours.

[0042] The titer of the fermentation broth was measured by the double-layer plate method, and the titer of phage PG124 was 2.60×10 10 pfu / ml. It shows that the culture medium of the present invention is also applicable to the expanded culture of Vibrio parahaemolyticus...

Embodiment 3

[0044] 1) Mix peptone 5.0g, yeast extract powder 1.0g, ferric citrate 0.1g, sodium chloride 19.45g, magnesium chloride 5.98g, sodium sulfate 3.24g, calcium chloride 0.3g, potassium chloride 0.55g, sodium carbonate 0.16g , potassium bromide 0.08g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride 0.0024g, disodium hydrogen phosphate 0.008g, water 1000ml, adjust the pH to 7.60, and sterilize at 121°C for 20 minutes.

[0045] 2) Vibrio alginolyticus J62 and the corresponding Vibrio alginolyticus phage PJ32 were inoculated in 100 ml of this medium and 100 ml of commercially available finished 2216E liquid medium in a certain proportion, and fermented for 5 hours at 37° C. and 170 rpm.

[0046] 3) The titer of the fermentation broth was determined by the double-layer plate method, and it can be seen that the titer was significantly improved.

[0047]

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Abstract

The invention provides a culture medium suitable for seawater phage fermentation and a preparation method and application thereof. The culture medium is prepared from the following components: 5.0 g of peptone, 1.0 g of yeast extract powder, 0.1 g of ferric citrate, 19.45 g of sodium chloride, 5.98 g of magnesium chloride, 3.24 g of sodium sulfate, 0.2-0.4 g of calcium chloride, 0.55 g of potassium chloride, 0.16 g of sodium carbonate, 0.08 g of potassium bromide, 0.022 g of boric acid, 0.004 g of sodium silicate, 0.0024 g of sodium fluoride and 0.008 g of disodium hydrogen phosphate in every 1000 mL of aqueous solution, and water serves as a solvent. When the culture medium is adopted for fermentation of vibrio bacteriophage, it can effectively improve the clarity of the culture medium and the titer of the bacteriophage fermentation liquor, has the advantages of low cost, simple preparation method and suitability for industrial production, and has good economic benefits.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method and production application of a culture medium formula suitable for Vibrio phage fermentation. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly and has made considerable profits. However, due to the widespread use of antibacterial drugs, it has brought about various problems such as environmental pollution, bacterial resistance, and food safety. In addition, antibiotics have disadvantages such as high cost and long research and development cycle, so there is an urgent need for a new antibacterial technology to solve this problem, which can control diseases without causing harm to the environment and human body, and partially or completely replace antibiotics. [0003] Bacteriophage (phage, bacteriophage) is a virus that parasitizes various bacteria, and is also called a bacterial virus. ...

Claims

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Application Information

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IPC IPC(8): C12N7/00
CPCC12N7/00C12N2795/00051Y02A40/81
Inventor 潘强任慧英孙虎芝庄盈婷白松庄莹
Owner QINGDAO PHAGEPHARM BIO TECH CO LTD
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