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Endophytic burkholderia gladioli PJB25 and application thereof

A technology of Burkholderia poplar and PJB25, applied to endogenous Burkholderia gladiolus PJB25 and its application fields, can solve the problem that the bacteriostatic effect needs to be improved and the like, and achieve easy storage and transportation, Environmentally friendly, fast reproduction effect

Active Publication Date: 2021-08-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chinese invention patent CN105018371A discloses a Dongxiang wild rice endophytic bacterium Burkholderia gladioli (Burkholderia gladioli) bacterial strain Fse32, and this bacterial strain is to rice sheath blight (Rhizoctoniasolani), wheat scab (Fusarium graminearum), Six kinds of plant pathogens, Sclerotinia scleriorum, Sclerotinialibertiana, Fusarium oxysporum, and Phytophthora capsici, all had antibacterial effects, but the inhibition rate was 30.6%~ 41.5%, of which the inhibitory rate to rice sheath blight is only 30.6%, and the antibacterial effect needs to be improved, and as a pure culture, the individual specificity of the strain is relatively strong, and it is greatly affected by the environment

Method used

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  • Endophytic burkholderia gladioli PJB25 and application thereof
  • Endophytic burkholderia gladioli PJB25 and application thereof
  • Endophytic burkholderia gladioli PJB25 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation, purification and preparation of endogenous Burkholderia gladiolus PJB2S and determination of antibacterial activity against peanut black rot

[0045] 1.1 Culture medium preparation

[0046] NA medium: peptone 10g, sodium chloride 5g, beef extract 3g, agar powder 20g, add water to make up to 1L; sterilize with damp heat at 121℃ for 20min for later use.

[0047] NB culture medium: peptone 10g, sodium chloride 5g, beef extract 3g, add water to make up to 1L; sterilize with damp heat at 121°C for 20min for later use.

[0048] LB medium: peptone 10g, sodium chloride 5g, yeast extract 5g, agar powder 20g, add water to 1L. 121°C damp heat sterilization for 20 minutes for later use.

[0049] PDA medium: Wash and peel the potatoes, weigh 200g of potatoes and cut them into small pieces, add water and boil until rotten (boil for 20-30min, it can be pierced by a glass rod), and filter with eight layers of gauze. Add 20g of glucose, add water to 1000mL, stir ...

Embodiment 2

[0059] Example 2 Molecular Identification of Endophytic Bacteria PJB25

[0060] 2.1 Culture medium preparation

[0061] The preparation of the culture medium is the same as 1.1 in Example 1.

[0062] 2.2 Molecular identification of endophytic bacteria PJB25

[0063] Using the endophytic bacteria PJB25 as a template, the bacterial genome DNA was extracted using the bacterial genome kit, and the 16SrRNA gene general primer fD2 / rP1 was used for PCR amplification.

[0064] Primer sequences: fD2 (5'-AGAGTTTGATCATGGCTCAG-3') and rP1 (5'-ACGGTTACCTTGTTACGACTT-3').

[0065] The PCR reaction system is:

[0066]

[0067] The PCR reaction conditions are:

[0068]

[0069] The amplified 16S rRNA target gene fragment was purified and connected to the pMD19-T vector, transformed into Escherichia coli DH5α, and the positive clones were entrusted to BGI for sequencing, sequence alignment was performed at NCBI, and a phylogenetic tree was constructed using MEGA5. The phylogenetic tr...

Embodiment 3

[0071] Embodiment 3 Endophytic bacteria PJB25 antibacterial spectrum is measured

[0072] 3.1 Preparation of medium

[0073] The preparation of the culture medium is the same as 1.1 in Example 1.

[0074] 3.2 Tested strains

[0075] Five important pathogenic fungi that harm peanut / soybean rhizome growth in production were selected as the test strains for the determination of the antibacterial spectrum of endophytic bacteria PJB25, including: peanut root rot fungus (Fusarium solani), peanut sheath blight fungus (Rhizoctonia solani), peanut Stem rot fungus (Lasiodiplodiatheobromae), peanut anthracnose fungus (Colletotrichumgloeosporioides) and peanut white silkworm fungus (Sclerotium rolfsii) were provided by the fungicide research laboratory of the Department of Plant Pathology, South China Agricultural University.

[0076] 3.3 Determination of antibacterial spectrum by plate confrontation method

[0077] Method is the same as 1.3 in embodiment 1.

[0078] The antibacterial...

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Abstract

The invention belongs to the technical field of biological control on plant diseases, and relates to an endophytic burkholderia gladioli PJB25 strain and an application thereof. According to the invention, the endophytic burkholderia gladioli PJB25 is separated and screened from healthy peanuts. The endophytic burkholderia gladioli PJB25 is safe and non-toxic. The endophytic burkholderia gladioli PJB25 is preserved in Guangdong Microbial Culture Collection Center on June 9, 2020, with preservation number of GDMCC No: 61053. The strain has a significant bacteriostatic effect on entry quarantine pests such as cylindrocladium parasiticum, and can significantly reduce the occurrence of black rot in peanuts. Meanwhile, the strain has an inhibiting effect on major fungal diseases such as peanut root rot, peanut sheath blight, peanut stem rot and peanut anthracnose threatening production of crops such as peanuts. The strain is wide in antibacterial spectrum, and provides a new choice for biological control on plant fungal diseases. The endophytic burkholderia gladioli PJB25 can be used for broad-spectrum microbial agents / bacterial fertilizers with good development and application prospects, and has important significance on green control on plant diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control, and in particular relates to an endophytic Burkholderia gladioli strain PJB25 and application thereof. Background technique [0002] Affected by biological or abiotic factors, plants undergo pathological changes in morphology, physiology, biochemistry, etc., causing plant diseases, which hinder their normal growth and development, and affect economic benefits. Common plant disease control methods include chemical control, physical control, biological control, and disease-resistant breeding. With the domestic emphasis on sustainable agriculture and environmental protection, more and more biological control methods are used in the prevention and control of plant diseases and insect pests. The biological control of plant diseases is the effective control of crop diseases by using beneficial microorganisms and microbial metabolites, among which the biological control of plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 纪春艳敖莉丝潘汝谦徐大高任董董
Owner SOUTH CHINA AGRI UNIV
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