A kind of Vibrio flexneri and its application in the preparation of multifunctional small tank fertilizer
A technology of Vibrio freundii and Vibrio freundii, applied in the field of Vibrio freundii and its application in the preparation of multifunctional small tank fertilizers, which can solve the problems of high requirements for production facilities and difficulty in miniaturized production, and achieve superior performance , stable performance, enhanced root growth and plant development
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Embodiment 1
[0044] Vibrio flexneri ( Vibrio fumissii ) isolation and identification
[0045] 1. Isolation of strains
[0046]The decayed Sargassum was collected from the intertidal zone, soaked in sterile water, and inoculated in liquid seawater medium for bacterial enrichment. The composition of liquid seawater medium was: peptone 10 g, yeast extract powder 5 g, seawater 1 L; then apply / stretch to the sodium alginate medium, the composition of the sodium alginate medium is: 10 g of sodium alginate, 5 g of ammonium sulfate. Dipotassium hydrogen phosphate 2 g, magnesium sulfate 1 g, ferrous sulfate heptahydrate 0.01 g, ultrapure water 1 L, incubate at 30°C for 24 h, observe the single colonies grown, and select the dominant bacteria that grow well on the sodium alginate plate Carry out numbering transfer; then stain the original plate with Gram's iodine solution to observe whether there is a transparent circle in each numbered strain. The appearance of a transparent circle means that it...
Embodiment 2
[0060] After activation, the single strain of Vibrio flexneri obtained through screening in the above examples was inoculated into the cooled seawater enrichment medium, and cultured with shaking at 30°C for 24 hours to obtain a single strain of Vibrio flexneri bacteria liquid.
[0061] The seawater enrichment medium: 10 g of peptone, 5 g of yeast extract powder, and 1 L of seawater were prepared and sterilized at 110°C for 30 min, cooled to room temperature before use.
[0062] In terms of parts by mass, add 100 parts of crushed dry Sargassum, 20 parts of Vibrio flexneri single-strain bacterial liquid, and 160 parts of water into the fermenter, control the temperature at 25°C in an unsealed state, and ferment for 7 days to obtain Fermentation material I, then add water into the fermenter, the mass-number ratio of fermentation material I to water is 1:3, stir evenly, soak and stand for 24 hours to obtain fermentation material II;
[0063] The fermented material II obtained abo...
Embodiment 3
[0068] A kind of multifunctional small pot fertilizer and preparation method thereof
[0069] The raw materials for preparing the multifunctional small pot fertilizer include by weight: 100 parts of dried sargassum after crushing, 5 parts of brown sugar, 20 parts of fine rice bran, 20 parts of compound microbial bacterial agent, and 160 parts of water;
[0070] The above crushed dried sargassum is obtained by crushing fresh sargassum salvaged by a crusher after drying in the sun.
[0071] The above-mentioned composite microbial bacterial agent is composed of Vibrio flexneri ( Vibrio fumissii ), Bacillus subtilis, Bacillus licheniformis, and Lactobacillus plantarum are directly mixed according to the weight ratio of each bacterial solution 1:1:1:1.
[0072] The Vibrio flexneri ( Vibrio fumissii ) was selected for the above example to obtain a single strain of Vibrio flexneri after activation, inoculate it into the cooled seawater enrichment medium, and culture it with shaki...
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