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Artificial amniotic bone synovial membrane and preparation method thereof

An amniotic membrane and synovial membrane technology, applied in the field of biomedical materials, can solve the problems of easy calcification, insufficient mechanical strength, and body support of biological bone synovium, and achieve the effects of improving mechanical strength, blocking calcium salt deposition, and improving support.

Active Publication Date: 2021-12-24
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biological bone synovium is easy to calcify, has poor durability, and has certain immunogenicity
At the same time, the current biological bone synovium is not strong enough to support the body

Method used

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  • Artificial amniotic bone synovial membrane and preparation method thereof
  • Artificial amniotic bone synovial membrane and preparation method thereof
  • Artificial amniotic bone synovial membrane and preparation method thereof

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preparation example Construction

[0031] Second aspect, the present invention also provides a kind of preparation method of artificial amniotic bone synovium, comprises the following steps:

[0032] S1. Soak the polymer biomaterial loaded with slow-release drugs in an aqueous solution containing carbodiimide and N-hydroxysuccinimide;

[0033] S2, soaking the amnion basement membrane or the amnion basement membrane solution in an aqueous solution containing amino group small molecular substances;

[0034] S3. Put the soaked polymer biomaterial, the soaked amnion basement membrane or the amnion basement membrane fluid in the amniotic membrane fluid, and obtain the artificial amniotic bone synovium after a cross-linking reaction.

[0035] The preparation method of the artificial amniotic bone synovium of the embodiment of the present application, the macromolecule biomaterial loaded with slow-release drugs is the intima layer; the amniotic basement membrane fluid is placed in an aqueous solution containing amino ...

Embodiment 1

[0054] The embodiment of the present application provides a method for preparing artificial amniotic bone synovium, comprising the following steps:

[0055] S1. Acquisition of amnion: Obtain the amnion in a sterile environment, soak it in saline containing penicillin, streptomycin and penicillin for 30 minutes, wash it several times to remove the blood, and then bluntly separate the amnion from the chorion of the placenta , spread its upper surface on a sterilized cellulose acetate membrane, and set aside;

[0056] S2. Acquisition of amniotic basement membrane: the amniotic membrane is separated from the fibroblast layer, spongy layer, epithelial cell layer and compact layer, then dehydrated and decellularized, and then washed with normal saline containing penicillin and streptomycin;

[0057] S3, dissolving gelatin, chitosan and polyvinyl alcohol in water to make a mixed solution, then adding a crosslinking agent and stirring evenly to prepare chitosan polymerized hydrogel; w...

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Abstract

The invention provides an artificial amniotic bone synovial membrane and a preparation method thereof. The artificial amniotic bone synovial membrane comprises an inner synovial membrane layer and an outer synovial membrane layer; the outer synovial membrane layer wraps the periphery of the inner synovial membrane layer; the space between the inner synovial membrane layer and the outer synovial membrane layer is filled with amniotic fluid; the inner synovial membrane layer is made of a polymer biomaterial loaded with sustained-release medicines; and the outer synovial membrane layer is an amniotic basement membrane or a liquefied and recured amniotic basement membrane liquid surface layer. According to the artificial amniotic bone synovial membrane, the inner synovial membrane layer is made of the polymer biomaterial loaded with the sustained-release medicines, and the outer synovial membrane layer is the amniotic basement membrane or the liquefied and recured amniotic basement membrane liquid surface layer; the sustained-release medicines loaded on the inner synovial layer act on the knee joint precisely; and the outer synovial membrane layer can realize rapid endothelialization and obstruct calcium salt deposition to improve the anti-calcification performance and keep the non-immunogenicity, and prevent permeation of blood components and tissue fluid components, calcium salt deposition and thrombus formation to prolong the service time.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an artificial amniotic bone synovium and a preparation method thereof. Background technique [0002] The synovium is the inner layer of the joint capsule, light red, smooth and shiny, thin and soft, composed of loose connective tissue. All structures in the joint cavity, except articular cartilage and meniscal cartilage plate, are wrapped by synovium. The synovial membrane secretes synovial fluid, which plays an important role in joint movement. The normal synovium is divided into two layers, a thin cellular layer (luminal layer) and a vascular layer (subintimal layer), attached to the non-articular surface and covering the bone surface in the joint capsule. The knee joint, as a part to maintain balance, enables people to use two feet to support the weight of a person who should originally be supported by four feet without falling down. Therefore, the knee joint is...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/20A61L27/22A61L27/24A61L27/54
CPCA61L27/3633A61L27/3604A61L27/24A61L27/225A61L27/222A61L27/20A61L27/3645A61L27/3687A61L27/54A61L2300/602A61L2400/18A61L2430/24
Inventor 周辉王月虹
Owner GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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