Preparation method of multifunctional electrospinning scaffold for bone regeneration

Abstract

The invention provides a preparation method of a multifunctional electrospinning scaffold for bone regeneration. The method belongs to the field of tissue engineering and regenerative medicine, and comprises the following specific steps: 1, preparing lysophosphatidic acid nanoparticles and deferoxamine nanoparticles; 2, preparing a medical polymer mixed solution and dividing the medical polymer mixed solution into two equal parts; 3, preparing a coaxial electrospinning shell layer solution from the lysophosphatidic acid nanoparticles, zinc oxide nanoparticles and the medical polymer mixed solution; 4, preparing a coaxial electrospinning core layer solution from the deferoxamine nanoparticles and another medical polymer mixed solution; 5, preparing the electrospinning scaffold with a core-shell structure; and 6, performing vacuum drying until a solvent is removed, thereby finally obtaining the product. According to the prepared multifunctional electrospinning scaffold for bone regeneration, the lysophosphatidic acid nanoparticles and the zinc oxide nanoparticles are arranged on the shell layer, the deferoxamine nanoparticles are arranged on the core layer, and the electrospinning scaffold has the functions of resisting bacteria, promoting osteogenesis, promoting revascularization and the like at the same time and can be used for repairing bone tissues in a complex microenvironment.

Description

Technical field [0001] The invention belongs to the technical field of tissue engineering and regenerative medicine, and relates to a method for preparing a multifunctional electrospun scaffold for bone regeneration. The prepared scaffold material also has the functions of antibacterial and promoting bone and blood vessel regeneration. Background technique [0002] Among the existing technologies, dental implants have attracted more and more attention as an effective way to restore function and achieve aesthetic restoration, and the clinical demand is strong. In 2011, the annual number of dental implants in my country was only about 120,000. From 2011 to 2017, the number of composite dental implants was With a growth rate of 56%, it is one of the fastest-growing dental implant markets in the world; the key to the success of dental implant technology is sufficient bone mass and soft tissue capable of proliferation at the implant site. However, due to trauma, periodontal disease...

AI Technical Summary

Problems solved by technology

[0004] At present, in the osteogenic application of deferoxamine, the patent "a polylactic acid-based bone tissue scaffold loaded with icariin and deferoxamine and its preparation method and application" uses surface-modified icariin and deferoxamine Amines can significantly improve the mechanical properties of the stent and are beneficial to the formation of the vascular network; the patent "An artificial periosteum loaded with deferoxamine and its preparation method" uses chemical bonds to bond DFO with amino groups to DFO-high Molecular materials are blended on the surface of artificial periosteum to achieve the effect of slow and long-lasting release; the patent "artificial drug-loaded coaxial regenerative vascular stent and its composite process preparation method" uses two processes to combine different materials to prepare a three-layer drug-loaded vascular stent, simulated The three-layer structure of natural blood vessels shortens the time required for in vitro culture and transplantation and the success rate; then, clinical bone defects are often accompanied by infection and inflammation, and how to deal with the complex microenvironment becomes the key to improving the ability of bone regeneration; currently, preparation Coaxial electrospun scaffolds that promote antibacterial, osteogenesis and angiogenesis have not been reported

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