Preparation method of multifunctional electrospinning scaffold for bone regeneration

A multi-functional, bone regeneration technology, applied in the direction of electrospinning, tissue regeneration, pharmaceutical formulations, etc., can solve the problems of shortening the transplantation time and success rate of in vitro culture, slow and long-lasting release, etc., and achieves controllable equipment cost and good stability , the effect of simple process

Active Publication Date: 2021-12-28
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the osteogenic application of deferoxamine, the patent "a polylactic acid-based bone tissue scaffold loaded with icariin and deferoxamine and its preparation method and application" uses surface-modified icariin and deferoxamine Amines can significantly improve the mechanical properties of the stent and are beneficial to the formation of the vascular network; the patent "An artificial periosteum loaded with deferoxamine and its preparation method" uses chemical bonds to bond DFO with amino groups to DFO-high Molecular materials are blended on the surface of artificial periosteum to achieve the effect of slow and long-lasting release; the patent "artif

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of multifunctional electrospinning scaffold for bone regeneration
  • Preparation method of multifunctional electrospinning scaffold for bone regeneration
  • Preparation method of multifunctional electrospinning scaffold for bone regeneration

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] A preparation method of a multifunctional electrospun scaffold for bone regeneration described in the present invention (the embodiment of the present invention discloses a preparation method of a three-dimensional multifunctional tissue engineering scaffold with antibacterial, osteogenic and angiogenesis-promoting), specifically The operation steps are as follows:

[0031] (1), prepare lysophosphatidic acid nanoparticles and deferoxamine nanoparticles respectively, and set aside for later use;

[0032] Specifically, the preparation method for preparing lysophosphatidic acid nanoparticles and deferoxamine nanoparticles is: the desolvation method can be selected to prepare albumin-coated lysophosphatidic acid nanoparticles;

[0033] Deferoxamine nanoparticles are prepared by two-step method to prepare lipid nanoparticles or organic solvent volatilization method to prepare nanoparticles such as liposomes;

[0034] In order to ensure sustained release, the prepared lysoph...

Embodiment 1

[0047] Nanoparticle preparation: Lysophosphatidic acid nanoparticles were prepared with lysophosphatidic acid as the active substance, albumin, chitosan, and absolute ethanol as auxiliary materials, with a particle size of about 250nm and a PDI of 0.23; deferoxamine as the active substance, phospholipids, Glycerol, SPAN80, and Tween 80 were used as excipients to prepare deferoxamine lipid nanoparticles, with a particle size of about 280 nm and a PDI of 0.18; the obtained nanoparticle solution was frozen at -80°C and then freeze-dried for 24 hours;

[0048] Preparation of electrospinning solution: Weigh polycaprolactone and polylactic acid copolymers and distribute them as 0.5g and 0.5g; add them into 6ml of hexafluoroisopropanol solvent, and stir evenly on a magnetic stirrer to obtain a polymer solution; Take 4.2ml of the solution, add 20mg of lysophosphatidic acid nanoparticles, add 23mg of zinc oxide nanoparticles, and stir until evenly distributed; at the same time, take 1.8...

Embodiment 2

[0052] Nanoparticle preparation: Lysophosphatidic acid nanoparticles were prepared with lysophosphatidic acid as the active substance, albumin, chitosan, and absolute ethanol as auxiliary materials, with a particle size of about 120nm and a PDI of 0.18; deferoxamine as the active substance, phospholipids, Glycerol and SPAN80 were used as auxiliary materials to prepare deferoxamine lipid nanoparticles with a particle size of about 336nm and a PDI of 0.21; the obtained nanoparticle solution was frozen at -80°C and then freeze-dried for 24 hours;

[0053] Preparation of electrospinning solution: Weigh 1g of silk fibroin; add it to 6ml of trifluoroethanol and dichloromethane mixed solvent, stir evenly on a magnetic stirrer to obtain a polymer solution; take 4.2ml of the solution and add lysophosphatidic acid nano 40 mg of particles, add 15 mg of zinc oxide nanoparticles, magnetically stir until evenly distributed; at the same time take 1.8ml of the solution, add 4 mg of deferoxamin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention provides a preparation method of a multifunctional electrospinning scaffold for bone regeneration. The method belongs to the field of tissue engineering and regenerative medicine, and comprises the following specific steps: 1, preparing lysophosphatidic acid nanoparticles and deferoxamine nanoparticles; 2, preparing a medical polymer mixed solution and dividing the medical polymer mixed solution into two equal parts; 3, preparing a coaxial electrospinning shell layer solution from the lysophosphatidic acid nanoparticles, zinc oxide nanoparticles and the medical polymer mixed solution; 4, preparing a coaxial electrospinning core layer solution from the deferoxamine nanoparticles and another medical polymer mixed solution; 5, preparing the electrospinning scaffold with a core-shell structure; and 6, performing vacuum drying until a solvent is removed, thereby finally obtaining the product. According to the prepared multifunctional electrospinning scaffold for bone regeneration, the lysophosphatidic acid nanoparticles and the zinc oxide nanoparticles are arranged on the shell layer, the deferoxamine nanoparticles are arranged on the core layer, and the electrospinning scaffold has the functions of resisting bacteria, promoting osteogenesis, promoting revascularization and the like at the same time and can be used for repairing bone tissues in a complex microenvironment.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and regenerative medicine, and relates to a method for preparing a multifunctional electrospun scaffold for bone regeneration. The prepared scaffold material has the functions of antibacterial, bone formation and blood vessel regeneration. Background technique [0002] In the prior art, as an effective way to restore function and achieve aesthetic restoration, dental implants have attracted more and more attention, and the clinical demand is strong. In 2011, the annual number of dental implants in China was only about 120,000. With a growth rate of 56%, it is one of the fastest-growing dental implant markets in the world; the key to the success of dental implant technology is to have sufficient bone mass and soft tissue capable of proliferating at the implant site, but due to trauma, periodontal disease infection, and tumor resection Various reasons lead to insufficient healthy bone mas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/54A61L27/58A61L27/02A61L27/26A61F2/28D01D5/34D01D5/00D01F1/10D01F8/02D01F8/14
CPCA61F2/28A61L27/54A61L27/58A61L27/025A61L27/26D01F8/14D01F8/02D01F1/10D01D5/34D01D5/003D01D5/0069A61F2002/2817A61F2002/2835A61F2002/3093A61F2002/30003A61F2002/3006A61F2240/001A61F2250/0067A61L2430/02A61L2300/624A61L2300/404A61L2300/412
Inventor 钱蕴珠邢丹蕾钱志远
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap