Paracoccus GN-9 and application thereof

A technology of GN-9 and Paracoccus, applied in the field of bioengineering, can solve the problems of changing soil nitrogen cycle, not being able to meet the demand of crop high yield to the maximum extent, and limited nitrogen, so as to promote plant growth, rich in carbon sources, and eco-friendly toxic effect

Active Publication Date: 2022-04-29
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrogen available in the soil is very limited and cannot meet the high yield requirements of crops to the maximum extent
Artificial application of nitrogen fertilizer is one of the most impor

Method used

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  • Paracoccus GN-9 and application thereof
  • Paracoccus GN-9 and application thereof
  • Paracoccus GN-9 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation and Identification of Bacteria

[0020] 1. Medium:

[0021] Ashby nitrogen-fixing culture medium (Ashby 1L): potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, calcium carbonate 5.0g, mannitol 10g, calcium sulfate 0.1g, with ddH 2 O to constant volume, adjust the pH to 7.0-7.5.

[0022] Nitrogen fixation medium (1L): mannitol 20g, potassium dihydrogen phosphate 0.2g, dipotassium hydrogen phosphate 0.8g, magnesium sulfate 0.2g, calcium sulfate 0.1g, yeast extract 0.5g, iron chloride trace, sodium molybdate trace, with ddH 2 O to constant volume, adjust the pH to 7.2.

[0023] 2. Source of the strain:

[0024] Azotobacter isolated from coral sand. Take 5g of the sample and put it into a 100ml centrifuge tube, add 50ml of sterile water to shake evenly, place it at room temperature, and let it stand until it becomes clear. The prepared soil suspension was gradiently diluted in an ultra-clean workbench, and 100 μL of the suspen...

Embodiment 2

[0031] Physiological and biochemical analysis of bacterial strains

[0032] The physiological and biochemical indicators of the strain GN-9 were tested using the Mérieux API 20NE non-fermenting G-bacteria identification kit.

[0033] GN-9 can utilize glucose, maltose, arabinose and mannose as the sole carbon source, but cannot utilize galactose.

[0034] Potassium nitrate reduction test was positive, indicating that GN-9 can reduce nitrate.

[0035] Oxidase and urea tests were all positive, indicating that the bacteria can decompose hydrogen peroxide and urea.

[0036] The detailed results are as follows:

[0037] Table 1 Physiological and biochemical properties analysis

[0038] Table 1 Analysis of Physiological and Biochemical Properties

[0039]

[0040] Note: +: Positive reaction; -: Negative reaction

Embodiment 3

[0042] Phosphate-solubilizing ability test results of strains

[0043] Phosphorus-dissolving medium (1L): glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.3g, magnesium sulfate 0.3g, manganese sulfate 0.03g, potassium sulfate 0.3g, ferrous sulfate 0.03g, tricalcium phosphate 5.0g, agar 15g.

[0044] Drop 6 μL of strain GN-9 on the phosphorus-dissolving medium, and after 5 days of growth, the size of the phosphorus-dissolving circle tends to be stable (such as figure 2 As shown), the colony is raised, light yellow, translucent, sticky, and the size of the phosphorus-dissolving circle is 37.08mm. It is proved that the strain has strong phosphorus-dissolving ability, that is, it can convert ineffective phosphorus that plants cannot absorb into available phosphorus that plants can absorb.

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Abstract

The invention belongs to the field of microbial resource development and utilization. The invention discloses a paracoccus sp. GN-9, and the preservation number of the paracoccus sp. GN-9 is CGMCC (China General Microbiological Culture Collection Center) No.23114. The paracoccus sp. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC for short) on August 5, 2021. The Paracoccus sp. GN-9 is short in culture period, free of ecological toxicity, rich in available carbon source variety, resistant to salt and alkali, capable of fixing nitrogen in a self-living mode, capable of decomposing insoluble phosphorus in soil and secreting indoleacetic acid and siderophores at the same time, capable of remarkably promoting growth of corn and rice and quite wide in application prospect in microbial fertilizer production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Background technique [0002] Nitrogen is the most critical nutrient element in the process of plant growth, and it is an important component of amino acids and proteins. The available nitrogen in the soil is very limited and cannot meet the high yield requirements of crops to the greatest extent. Artificial application of nitrogen fertilizer is one of the most important measures to achieve high crop yield, but excessive nitrogen fertilizer will change the soil nitrogen cycle, significantly reduce microbial biomass nitrogen, and lead to increased salinization. Excessive enrichment of salt ions and high pH value not only cause osmotic stress, water loss and poisoning of plant cells, but also affect the activities of microorganisms, restrict the transformation and supply of nitrogen and other nutrients, resulting in a lack of available nutrients in the soil . [0003] In agroecosystems, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/80C05F11/08C09K17/14C12P17/10C12P1/04C09K101/00C09K109/00C12R1/01
CPCC05F11/08C05G3/80C09K17/14C12P17/10C12P1/04C09K2101/00C09K2109/00
Inventor 仲乃琴曹晶晶赵盼汪志琴赵永龙刘璐王秀
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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