Three-dimensional porous tissue engineering stand material and preparation thereof

A tissue engineering scaffold, three-dimensional porous technology, applied in the field of biomedical materials-tissue engineering scaffold materials, can solve the problems of unsatisfactory biodegradability, lack of spatial structure and mechanical strength of natural polymers, etc., and achieves low cost and good biological properties. Activity and degradability, wide-ranging effects

Inactive Publication Date: 2006-10-11
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of materials have been used as bioscaffold materials, such as biopolymer materials, bioceramics / glass, etc., but most of these materials have different shortcomings, and it is difficult to fully meet the requirements of clinical applications.
For example, synthetic polymers (such as PLA, etc.) will have a chronic reaction with the body, and aseptic inflammation will appear after degradation; natural polymers (such as chitosan, collagen, etc.) lack the necessary spatial structure and mechanical strength; bioceramic / glass materials (such as β-calcium phosphate biomaterials, calcium silicate materials, etc.) have special biological activity (good combination with natural bone), but the biodegradability is not satisfactory

Method used

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  • Three-dimensional porous tissue engineering stand material and preparation thereof
  • Three-dimensional porous tissue engineering stand material and preparation thereof
  • Three-dimensional porous tissue engineering stand material and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] With 1M acetic acid solution preparation concentration is that the gelatin solution of 15% and the chitosan solution that concentration is 1%, then by 1: 1 it is uniformly blended, and obtaining gelatin, chitosan content are respectively (by mass) 7.5%, 0.5% gelatin / chitosan mixed solution. Add GPSM and calcium nitrate to the above solution respectively, wherein GPSM / calcium nitrate is (by mass) 1 / 0.05; GPSM / (gelatin+chitosan) is (by mass) 0.5 / 1. The above solution was fully mixed for 4 hours at 40°C until it was completely uniform (no phase separation), and then 25ml of it was injected into a 5cm×8cm mold, sealed, and placed in a thermostat at 40°C for 3 days to obtain gelatin-chitopolymer Sugar-calcium silicate gel. Add 10 ml of 1M ammonia solution to the above-mentioned mold, let it stand at 25° C. for 16 hours, and then fully wash it with 1M NaCl solution and distilled water until neutral. Pre-freeze the ammonia-treated gel at -20°C for 24 hours, and then freeze-d...

Embodiment 2

[0047] Use 0.1M hydrochloric acid solution to prepare a gelatin solution with a concentration of 10%, add GPSM and calcium nitrate to the above solution respectively, wherein GPSM / calcium nitrate is (by quality) 1 / 0.05; GPSM / gelatin is (by quality) 0.5 / 1 . The above solution was fully mixed at 40°C for 4 hours until it was completely uniform (no phase separation), and then 25ml of it was injected into a 5cm×8cm mold, sealed, and placed in a thermostat at 40°C for 3 days to obtain gelatin-silicic acid calcium gel. Add 10 ml of 1M ammonia solution to the above-mentioned mold, let it stand at 25° C. for 16 hours, and then fully wash it with 1M NaCl solution and distilled water until neutral. The ammonia-treated gel was pre-frozen at -20°C for 24 hours, and then freeze-dried at -40°C at 5KPa for more than 48 hours. After freeze-drying, a gelatin-calcium silicate three-dimensional porous scaffold could be obtained. The porosity of the obtained porous material was tested by the Ar...

Embodiment 3

[0049] With 1M acetic acid solution preparation concentration is that the gelatin solution of 15% and the chitosan solution that concentration is 1%, then by 1: 1 it is uniformly blended, and obtaining gelatin, chitosan content are respectively (by mass) 7.5%, 0.5% gelatin / chitosan mixed solution. Add GPSM and calcium nitrate to the above solution respectively, wherein GPSM / calcium nitrate is (by mass) 1 / 0.05; GPSM / (gelatin+chitosan) is (by mass) 0.5 / 1. The above solution was fully mixed for 4 hours at 40°C until it was completely uniform (no phase separation), and then 25ml of it was injected into a 5cm×8cm mold, sealed, and placed in a thermostat at 40°C for 3 days to obtain gelatin-chitopolymer Sugar-calcium silicate gel. Add 10 ml of 1M ammonia solution to the above-mentioned mold, let it stand at 25° C. for 16 hours, and then fully wash it with 1M NaCl solution and distilled water until neutral. Pre-freeze the ammonia-treated gel in liquid nitrogen (-196°C) for 2 hours,...

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Abstract

A three-dimensional porous tissue engineering scaffold material and a preparation method thereof relate to a biomedical material with good biological activity and degradability-a tissue engineering scaffold material. It is composed of chitosan, gelatin, GPSM and calcium nitrate. It is prepared by sol-gel, chemical treatment and freeze drying. The physical and chemical properties and degradation rate of the material are controlled by controlling the blending ratio of collagen / chitosan / GPSM / calcium nitrate, reaction temperature, time and other factors; the microstructure of the material is controlled by adjusting the freezing temperature and other factors. Bioactivity and degradability, suitable porosity and pore size, and can compound growth factors that play a vital role in tissue construction to induce osteoblast differentiation, which is conducive to the formation of bone tissue, and can meet the needs of the new generation of cartilage and bone. The need for tissue engineering scaffold material development.

Description

technical field [0001] The invention relates to a biomedical material-tissue engineering scaffold material with good biological activity and degradability. Background technique [0002] Since the 19th century, bone grafting has been committed to repairing large-scale bone defects caused by trauma, tumors, and infections to restore limb function. But there are many problems with this method, such as immune rejection and shortage of donor tissues and organs. An ideal strategy is to artificially cultivate tissues and organs, and then put forward the concept of tissue engineering, whose goal is to use the composite culture of cells and biological scaffold materials to regenerate bone tissue and achieve the purpose of repair. Among them, on the one hand, the scaffold material serves as a carrier of signal molecules or cells to deliver them to the defect site, and on the other hand, it provides a scaffold for new bone growth. At present, a variety of materials have been used as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/54A61L27/56A61L27/58A61L27/44
Inventor 任磊张其清
Owner XIAMEN UNIV
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