A protective agent for resisting UV-B radiation damage

A radiation damage and protective agent technology, applied in the field of chemistry, can solve the problems of side effects of organic synthetic preparations, etc.

Inactive Publication Date: 2006-12-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although organically synthesized anti-ultraviolet radiation preparations have the advantages of good effect and low production...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Dilute protective agent 1 with phosphate buffer solution at a concentration of 50 mg / L, and co-culture with JB6 monolayer skin cells for 1 hour, and carry out an intensity of 110 μW / cm 2 For UV-B treatment, the radiation time was 30 minutes, and the JB6 cells directly treated with UV-B radiation without co-cultivation with this preparation were used as a control. Physiological indicators and gene expression detection were performed after the treatment. The results showed that after UV-B treatment, the lipid peroxidation level of JB6 cells increased by more than 30%, and the gene expression level of cyclooxygenase 2 was significantly enhanced; the phosphorylation level and enzyme activity of protein kinase B and phosphoinositide-3-kinase were also significantly Increase. Cyclooxygenase 2 gene expression is one of the main indicators of cell carcinogenesis; at the same time, protein kinase B and phosphoinositide-3-kinase are also important signal transduction proteins in...

Embodiment 2

[0017] After the protective agent 3 was diluted with 250mg / L aqueous solution and sprayed on Arabidopsis seedlings, the intensity was 190μW / cm 2 UV-B radiation treatment for 1h. The results show that only spraying distilled water and not spraying the Arabidopsis thaliana formulated by the present application will cause obvious wilting symptoms, and at the same time, related enzymes of flavonoid metabolic pathways (such as dihydroflavone dehydrogenase, chalcone synthase, etc.) The intensity of gene expression increased significantly, and the enhancement of gene expression related to flavonoid metabolic pathways was the self-defense response of Arabidopsis against UV-B stress; the Arabidopsis plants that were sprayed with the combination of this application and then irradiated with UV-B stood upright Good sex, no obvious damage symptoms, flavonoid metabolic pathway-related gene expression intensity and normal growth plants (4000μmol / cm 2 / s white light irradiation) there was no...

Embodiment 3

[0019] Dilute the protective agent 4 with physiological saline to contain 50mg / L, pretreat the lens of rats cultured in vitro for 1h, and then apply 25μW / cm 2 UV-B radiation treatment for 15 minutes, the normal saline without this preparation was pre-incubated and UV-B radiation was used as a positive control, and the UV-B radiation treatment was not used as a negative control. The results showed that, compared with the negative control, the apoptosis rate of isolated rat lens cells subjected to UV-B radiation after pre-incubation with normal saline increased by 18%, the degree of cross-linking of crystal proteins exceeded 50%, and the integrity of DNA was also significantly reduced. The apoptosis rate of lens cells, the increase of crystal protein cross-linking degree and the decrease of DNA integrity are important indicators of cataract formation. However, after UV-B radiation, the rat lens pre-cultured with 50 mg / L of this preparation, the apoptosis rate, DNA damage and cry...

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Abstract

Disclosed is a protective agent for resisting UV-B radiation injury, which is a composition extracted and purified from tea. The active constituents of the composition include (by weight percent): epigallocatechin-3-gallate (EGCG) 40-65%, epicatechin-3-gallate (ECG)15-25%, and balancing adjuvant.

Description

technical field [0001] The invention belongs to the field of chemistry, and relates to a composition of natural products extracted from plants, in particular to a protective agent capable of efficiently resisting UV-B radiation damage. Background technique [0002] Solar ultraviolet radiation refers to electromagnetic waves with a wavelength range of 150-380nm, among which ultraviolet radiation with a wavelength of less than 280nm is called short-wave ultraviolet or UV-C, and ultraviolet radiation with a wavelength of 280-320nm is called medium-wave ultraviolet or UV-B. Ultraviolet radiation at 320 nm is called long-wave ultraviolet or UV-A. There are three kinds of ultraviolet rays in solar radiation: UV-A, UV-B and UV-C. UV-C radiation has a strong killing effect on organisms, but it can only travel in a vacuum. When it passes through the atmosphere, it is strongly absorbed by the ozone in the stratosphere and rarely reaches the surface; UV-A and UV-B can The UV-B that r...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61K8/97A61K9/00A61P17/16A61P27/02A61Q17/00
Inventor 陆建良梁月荣金晶郑新强
Owner ZHEJIANG UNIV
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