Beta hydroxy short to medium chain fatty acid polymer

Abstract

The present invention provides a composition comprising a polymer of a β-hydroxy short-medium chain fatty acid, which is used for delivering the β-hydroxy short-medium chain fatty acid or an oligomer thereof to the large intestine. In case the composition is administrated orally, the composition will be delivered to the large intestine, without being degraded in the stomach or short intestine, and degraded by the large intestinal bacterial flora and release the short-medium chain fatty acid or an oligomer thereof. The released short-medium chain fatty acid or an oligomer thereof has useful physiological activities and is effective for treating or preventing inflammatory diseases or cancer in the large intestine.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for delivering a β-hydroxy short-medium chain fatty acid monomer or an oligomer thereof to the large intestine by means of oral administration or a procedure parallel to oral administration. The present invention further relates to a composition which is used for delivering a β-hydroxy short-medium chain fatty acid monomer or an oligomer thereof to the large intestine of a subject by means of oral administration or a procedure parallel to oral administration. [0002] Further, the invention relates to a novel animal feeding stuff. [0003] Further more, the invention relates to a pharmaceutical composition and a functional food product useful for keeping or improving physiologic function, for treating or preventing inflammatory bowel disease and for treating or preventing large intestine cancer. [0004] Still further, the invention relates to a coating composition useful for delivering an active ingredient to the large inte...

AI Technical Summary

Benefits of technology

[0204] The average stool frequency observed in the test group of 32.5 was significantly larger (+30%) than that of the control group of 25. This supports the effect of the invention to activate the intestinal movement and provide better excretion.

[0205] The urination frequency in both groups was about 40 and there were no significant difference between the groups. The average amount of the urine in the test group of 3,946 g was non-significantly lower (−11%) than that in the control group of 4,452 g, Further, the average water content in the feces of the test group of 62.6% was significantly lower (−10%) than that in the control group of 69.4%.

[0206] As is discussed above, the new findings concerning the effects of the present invention including the increase in stool frequency and decrease in the amount of urine were obtained. TABLE 5Result of Feeding Pigs in the Metabolite Cage (Five Days)control grouptest group (SD)(SD)diet intake (kg) 7.14(0.44)**6.41(0.34)body weight gain (kg)3.0(1.0)2.9(0.8)water intake (kg)16.1(3.0) 16.2(1.0) feed conversion ratio kg / kg2.51(0.60)2.24(0.65)stool frequency 32.5(3.4)**25.0(4.0) fecal amount g1,751(207)  1,367(362)  urination frequency40.3(5.1) 41.5(9.4) urine amount (g)3,946(132)* 4,452(499)  water content in the feces 62.6(2.0)**69.4(1.1) (wt %)*p < 0.1 **p < 0.05 Effects on the Nitrogen Balance

[0207] The nitrogen balance was calculated based on the nitrogen amount in the feces and urine determined by the Kjeldahl method, dried ratio of the diet (89.5%), CP value of the diet (26.0%) and the nitrogen amount conversion factor (6.25). Results are summarized in Table 6.

[0208] There were no significant difference in the nitrogen intake and nitrogen excretion in the feces and urine. However, there was an increasing trend in the test group with respect to the amount of the nitrogen accumulation in the body. It was 172.0 g in the control group whereas 189.6 in the test group. The body accumulation ratio in the test group was suggested larger than the control group. In order to confirm the suggestion, the results are expressed in percentage in Table 7. According to table 7, some trends were observed in the test group, namely: the nitrogen accumulation in the body was increased and the nitrogen excretion was decreased, and the ratio of the nitrogen excreted in the urine to that in the feces was reduced (i.e. the ratio excreted in the feces was increased). That is, the composition of the present invention is effective for improving feed conversion ratio and increasing muscle amount as well as decreasing urine disposal cost. It is more difficult to treat nitrogen contents in the urine than those in the feces. TABLE 6Nitrogen Balance Test in Pigstest groupcontrol group(SD)(SD)nitrogen intake (g)252.7(15.6)238.5(12.4)total nitrogen excretion63.1(4.2)66.5(4.7)(g)nitrogen accumulation 189.6(13.6)*172.0(7.1) (g)fecal nitrogen (g)19.5(0.8)19.4(1.1)urinary nitrogen (g)43.7(4.0)47.1(3.5)*p < 0.1 **: p < 0.05

[0209]TABLE 7Calculated Nitrogen Balance Test Resultstest groupcontrol group(SD)(SD)nitrogen accumulation75.0(1.4)*72.1(2.3)rate %total nitrogen excretion25.0(1.4)*27.9(2.3)rate (%)fecal nitrogen excretion7.7(0.4) 8.1(0.6)rate (%)urinary nitrogen17.3(1.4)*19.8(1.6)excretion rate (%)excretion ratioin feces30.9(1.7)*29.1(0.3)in feces orin urine69.1(1.7)*70.9(0.3)urine (%)*<0.1 **: <0.05 EXAMPLE 7 Inflammatory Preventing Effect

Problems solved by technology

However, all of thus administrated resistant carbohydrates are not necessarily converted to short chain fatty acids.

The short chain fatty acid production efficiency from the resistant carbohydrates are generally low.

In addition, the fermentation speed of the resistant carbohydrates is greatly affected by the manner of intake or the existence form of the same in the food.

The other organic acids may affect badly.

For example, production or accumulation of a large amount of lactic acid may cause diarrhea.

However, such procedure allows only intermittent administration.

It is difficult to deliver the acid to entire large intestine by the procedure because there are some parts in the large intestine where the acid cannot reach.

In addition, it is also difficult to keep the effective concentration of the injected short chain fatty acid in the large intestine for long time.

To date, no practical procedure for delivering the short-medium chain fatty acid to the large intestine in a controlled manner has been developed.

However, they are silent about physiological effects of the copolymer on the sheep.

Further, there are some problems in manufacturing the coatings due to the poor solubility of the used polymers in the less-residual volatile organic solvent.