Multifunctional dry plate test board and preparation method and application thereof

A detection board, a multifunctional technology, applied in the preparation of test samples, biochemical equipment and methods, biological testing, etc., can solve the problems of low detection efficiency, not very wide application, hindering the development and comprehensive popularization of detection technology, etc. Achieve the effects of high degree of automation, fast detection speed, scientific design and development and technical route

Inactive Publication Date: 2009-01-28
吕炜锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, dry (bio)chemical rapid detection technology is still an emerging technology in the field of clinical detection, and it is not widely used at home and abroad. Single-it

Method used

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  • Multifunctional dry plate test board and preparation method and application thereof
  • Multifunctional dry plate test board and preparation method and application thereof
  • Multifunctional dry plate test board and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1 ALT dry film detection board

[0023] The ALT dry slice detection plate is based on the pyruvate oxidase method to quantitatively detect alanine aminotransferase in human blood samples. First, ALT catalyzes the conversion of alanine and α-ketoglutarate to pyruvate and glutamic acid. Pyruvate and phosphate, O 2 When oxidized under the catalysis of pyruvate oxidase, acetyl phosphate and hydrogen peroxide are generated. The generated hydrogen peroxide has strong oxidizing properties and can oxidize the reducing color developer to produce color changes. The reaction formula is as follows:

[0024]

[0025]

[0026]

[0027] Each test plate contains the following biochemical reaction reagents:

[0028] L-alanine 168~252μg

[0029] α-ketoglutarate 15.7~25.6μg

[0030] TPP 10.9~17.4μg

[0031] MgCl 2 1.7~2.4μg

[0032] Pyruvate oxidase 1.9~2.8U

[0033] Peroxidase 18.0~26.3U

[0034] Chromogenic agent 24.8~35.6μg

[0035] Preparation of ALT dry sli...

Example Embodiment

[0043] Example 2 (Detection board for blood lipids and blood sugar dry tablets)

[0044] The blood lipid and blood sugar dry film detection board is based on the cholesterol oxidase method, the glycerol phosphate oxidase method, and the glucose oxidase method to quantitatively detect the total cholesterol, high-density lipoprotein, triglycerides and glucose in human blood samples. The reaction equations are as follows:

[0045]

[0046]

[0047]

[0048]

[0049]

[0050]

[0051]

[0052]

[0053]

[0054] Each test plate contains the following biochemical reaction reagents:

[0055] Cholesterol esterase 0.1~0.9U

[0056] Cholesterol oxidase 0.01~0.07U

[0057] Glycerin kinase 0.2~0.7U

[0058] Phosphoglycerol oxidase 0.1~0.9U

[0059] Horseradish peroxidase 0.1~0.5U

[0060] MgCl 2 1.7~2.4μg

[0061] Glucose oxidase 0.2~1U

[0062] ATP 18~26μg

[0063] Chromogenic agent 50~120μg

[0064] Preparation of blood lipids and blood sugar dry tablets

...

Example Embodiment

[0071] Example 3 Renal function (creatinine, urea nitrogen, blood potassium, carbon dioxide content) detection board

[0072] The detection principle of creatinine is based on the fact that creatinine produces creatine under the action of creatinase, creatine produces creatine under the action of creatinase, and finally creatine produces hydrogen peroxide under the action of creatine oxidase, thereby oxidizing the color reagent Color rendering.

[0073]

[0074]

[0075]

[0076]

[0077] The detection principle of urea nitrogen is based on the formation of ammonia under the action of urease, and the colour of ammonia under the action of sodium hypochlorite and sodium salicylate.

[0078]

[0079]

[0080] The detection principle of serum potassium is based on the ionophore (2,3-naphtho-15-crown-5) in the reaction system that once specifically binds to potassium ions, the reporter substrate (7-decyl-MEDPIN) will lose protons and change Its color.

[0081] The detection...

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Abstract

The invention provides a multifunctional dry plate checkout console, and a preparation method and application thereof, belonging to the field of medical instrument. The multifunctional dry plate checkout console comprises a solid reaction carrier film (1) used for adsorbing biochemical reagent, a carbon fibre bar frame (2), a supporting pad (5) with the upper layer attached by thin fibreglass paper (3), a hard bracket (7) added by whole blood filter paper (4), and an elastic pad (6). The product of the invention is a disposable medical detection consumable belonging to quick micro-detection, can quantitatively/qualitatively and quickly detect a plurality of biochemical indexes in human body blood samples (whole blood, blood plasma and serum), with the detection process completed within 5 minutes; the preparation method and the application thereof have important significance for critical quick quantitative detection and disease diagnosis.

Description

technical field [0001] The invention relates to a multifunctional dry film detection board, its preparation method and its application in medical diagnosis. Specifically, it relates to the structure, preparation method, and application of a multi-functional dry film detection board capable of rapid quantitative or qualitative simultaneous detection of multiple biochemical indicators, and its application in medical testing and clinical diagnosis. Background technique [0002] Medical examination has always been one of the most accurate and effective means of disease diagnosis. Today, with the rapid development of medical testing technology, people can use the advanced technology of various disciplines to serve clinical testing and diagnose diseases in a timely and effective manner. Since the end of the last century, people have applied fully automatic biochemical instruments integrating life science technology, electronic technology, computer technology and other advanced te...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N1/28G01N21/78C12Q1/00
Inventor 吕炜锋蒋昵真郝曦东
Owner 吕炜锋
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