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Multifunctional dry plate test board and preparation method and application thereof

A detection board, a multifunctional technology, applied in the preparation of test samples, biochemical equipment and methods, biological testing, etc., can solve the problems of low detection efficiency, not very wide application, hindering the development and comprehensive popularization of detection technology, etc. Achieve the effects of high degree of automation, fast detection speed, scientific design and development and technical route

Inactive Publication Date: 2009-01-28
吕炜锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, dry (bio)chemical rapid detection technology is still an emerging technology in the field of clinical detection, and it is not widely used at home and abroad. Single-item detection has low detection efficiency, which is also an important reason for hindering the further development and comprehensive popularization of dry (bio)chemical detection technology

Method used

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  • Multifunctional dry plate test board and preparation method and application thereof
  • Multifunctional dry plate test board and preparation method and application thereof
  • Multifunctional dry plate test board and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 ALT dry film detection plate

[0023] The ALT dry film detection panel is based on the pyruvate oxidase method for the quantitative detection of alanine aminotransferase in human blood samples. First, ALT catalyzes the transformation of alanine and α-ketoglutarate into pyruvate and glutamic acid, and pyruvate and phosphate, O 2 Under the catalysis of pyruvate oxidase, it is oxidized to generate acetyl phosphate and hydrogen peroxide, and the generated hydrogen peroxide has strong oxidizing property, which can oxidize the reductive color developing agent to produce color change. The reaction formula is as follows:

[0024]

[0025]

[0026]

[0027] Each assay plate contains the following biochemical reagents:

[0028] L-alanine 168~252μg

[0029] α-ketoglutarate 15.7~25.6μg

[0030] TPP 10.9~17.4μg

[0031] MgCl 2 1.7~2.4μg

[0032] Pyruvate oxidase 1.9~2.8U

[0033] Peroxidase 18.0~26.3U

[0034] Chromogen 24.8~35.6μg

...

Embodiment 2

[0043] Embodiment 2 (blood fat, blood sugar dry film detection board)

[0044] The blood lipid and blood sugar dry film test board is based on the cholesterol oxidase method, the phosphoglycerol oxidase method, and the glucose oxidase method for the quantitative detection of total cholesterol, high-density lipoprotein, triglyceride and glucose in human blood samples. Its reaction equation is as follows respectively:

[0045]

[0046]

[0047]

[0048]

[0049]

[0050]

[0051]

[0052]

[0053]

[0054] Each assay plate contains the following biochemical reagents:

[0055] Cholesterol esterase 0.1~0.9U

[0056] Cholesterol oxidase 0.01~0.07U

[0057] Glycerol kinase 0.2~0.7U

[0058] Phosphate glycerol oxidase 0.1~0.9U

[0059] Horseradish peroxidase 0.1~0.5U

[0060] MgCl 2 1.7~2.4μg

[0061] Glucose oxidase 0.2~1U

[0062] ATP 18~26μg

[0063] Chromogen 50~120μg

[0064] Preparation of blood lipid and blood sugar dry fil...

Embodiment 3

[0071] Example 3 Renal function (creatinine, blood urea nitrogen, blood potassium, carbon dioxide content) detection board

[0072] The detection principle of creatinine is based on the fact that creatinine generates creatine under the action of creatinase, creatine generates sarcosine under the action of creatinase, and finally sarcosine generates hydrogen peroxide under the action of sarcosine oxidase, so that the chromogenic agent is oxidized color.

[0073]

[0074]

[0075]

[0076]

[0077] The detection principle of urea nitrogen is based on the fact that urea generates ammonia under the action of urease, and ammonia develops color under the action of sodium hypochlorite and sodium salicylate.

[0078]

[0079]

[0080] The detection principle of serum potassium is based on the fact that once the ionophore (2,3-naphtho-15-crown-5) in the reaction system specifically binds to potassium ions, the reporter substrate (7-decyl-MEDPIN) will lose a proton, th...

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Abstract

The invention provides a multifunctional dry plate checkout console, and a preparation method and application thereof, belonging to the field of medical instrument. The multifunctional dry plate checkout console comprises a solid reaction carrier film (1) used for adsorbing biochemical reagent, a carbon fibre bar frame (2), a supporting pad (5) with the upper layer attached by thin fibreglass paper (3), a hard bracket (7) added by whole blood filter paper (4), and an elastic pad (6). The product of the invention is a disposable medical detection consumable belonging to quick micro-detection, can quantitatively / qualitatively and quickly detect a plurality of biochemical indexes in human body blood samples (whole blood, blood plasma and serum), with the detection process completed within 5 minutes; the preparation method and the application thereof have important significance for critical quick quantitative detection and disease diagnosis.

Description

technical field [0001] The invention relates to a multifunctional dry film detection board, its preparation method and its application in medical diagnosis. Specifically, it relates to the structure, preparation method, and application of a multi-functional dry film detection board capable of rapid quantitative or qualitative simultaneous detection of multiple biochemical indicators, and its application in medical testing and clinical diagnosis. Background technique [0002] Medical examination has always been one of the most accurate and effective means of disease diagnosis. Today, with the rapid development of medical testing technology, people can use the advanced technology of various disciplines to serve clinical testing and diagnose diseases in a timely and effective manner. Since the end of the last century, people have applied fully automatic biochemical instruments integrating life science technology, electronic technology, computer technology and other advanced te...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N1/28G01N21/78C12Q1/00
Inventor 吕炜锋蒋昵真郝曦东
Owner 吕炜锋
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