Protein involving self-incompatibility and cross-compatibility control of phanerogam pollen, coding gene thereof and application

Abstract

The invention discloses protein involving self-incompatibility and cross-compatibility control of phanerogam such as Solanaceae plant pollen, coding gene thereof and an application of reducing the gene expression through transgene on aspects of preventing transgene flow and establishing a gene bank of a variety. The invention provides PhSSK1-RNAi carrier and a host cell containing the carrier. The gene of the present invention has an important theoretical significance in revealing molecule mechanism of self-incompatibility of the phanerogam such as Solanaceae plant. Through transgene RNAi, reducing the gene expression can cause pollen to loose compatibility in cross-pollination, having an important effect and meaning on preventing transgene flow of Solanaceae transgene plant and establishing a good trait control gene bank of Solanaceae economic crop such as potato, tomato and the like.

Description

field of invention [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a protein related to flowering plants, such as Solanaceae class self-incompatibility pollen outcross-compatibility control and its coding gene SSK1, as well as using transgenic RNAi to down-regulate the expression of this gene in pollen. It plays the function of pollen outcross compatibility loss; meanwhile, it also involves the comparative analysis of the protein sequence encoded by the gene, etc. Background technique [0002] Transgenic technology is one of DNA recombination technologies. Since the first success of transgenic plants in tobacco in 1983, there have been more than 120 kinds of plants in 35 families. Because the transgenic technology can make the crops have excellent quality and attributes that they did not have originally through the introduction of foreign genes, it can increase crop yield, improve quality, reduce the use...

AI Technical Summary

Problems solved by technology

First, GM crops may pose environmental and health biosafety concerns

Genetically modified crops often carry resistance selection genes, and it has been suggested that these resistance markers may affect the therapeutic effect of antibiotic drugs in humans

In addition, most of the target genes transferred into genetically modified crops are insect-resistant and disease-resistant genes, and the method of genetic modification is usually mediated by pathogenic bacteria or viruses. Therefore, the outflow of pathogenic bacteria and viruses carrying these genes may generate new pathogenic virus [3]

Second, genetically modified crops may cause ecological security risks

The resistance genes of transgenic crops with herbicide resistance may be transferred to weeds or related wild-type varieties through gene flow between species, making these weeds and wild species resistant to herbicides, many times Can become a "superweed" after transfer, which can damage the ecosystem [4-6]

In addition, transgenic crops as exotic varieties usually have a "selective advantage", which may affect the genetic structure of the plant gene pool, resulting in the loss of wild alleles and other genetic resources, and then affect the types of species and populations in the ecosystem. amount, resulting in loss of biodiversity [6]

Third, genetically modified crops may cause safety problems of genetically modified food

Thus, it can be seen that it is an effective method to limit the gene flow of transgenic plants by producing male sterile lines through transgenic methods

However, in these lines where male sterile lines have been constructed, although the resulting male sterile lines significantly limit the drift of transgenes, it also makes it impossible for the obtained transgenic lines to self-fertilize, so it is impossible to obtain corresponding Homozygous, not conducive to breeding

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