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Preparation method of high-purity mannan oligosaccharide

A mannan oligosaccharide and mannanase technology, applied in the field of konjac manna oligosaccharide production, can solve the problem of increasing enzymatic hydrolysis raw material induction and subsequent application instructions, affecting product yield and production cost, and not simplifying and improving the process and other problems, to achieve the effect of reducing cleaning frequency, good anti-fouling ability and high operating pressure

Active Publication Date: 2013-12-25
ENSHI TINJOY BIO TECH
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Problems solved by technology

[0005] Chinese patent application No. 200710051436.3 "Production method of high-purity mannan oligosaccharides" discloses that mannanase is used to enzymatically hydrolyze konjac fine powder, and then undergo centrifugal filtration, diatomite filtration, microfiltration, ion exchange, and ultrafiltration. , Vacuum drying, and spray drying to produce mannan oligosaccharides, the enzyme activity is average, the substrate concentration is 10-20%, the ratio of enzyme and substrate is 1:10-20 (v / w), the biological activity of glucomannan in vegetable gum The conversion rate is 70-90%. Diatomaceous earth filtration and microfiltration are used in the process, resulting in large loss, vacuum concentration, and high energy consumption.
Affect product yield and production cost
Chinese Patent Application No. 201110305324 "Research on the Preparation of High-purity Mannan Oligosaccharides by Enzymolysis of Hemicellulose" is basically the same as the method disclosed in Application No. 200710051436.3, but the introduction of enzymolysis raw materials and subsequent application instructions are added, and there is no simplification and improvement in the process
[0006] The above technologies all use plant gums such as konjac powder and guar gum as raw materials, and prepare mannan oligosaccharides by mannanase enzymolysis. Big, the sugar liquid is turbid, need to use acid and alkali to adjust the pH, the pollution is large, it can only be limited to the laboratory stage, and cannot be used for industrial production
According to the patent, neutral β-mannanase is mainly used to prepare mannan oligosaccharides, but the enzyme activity is low, the substrate concentration is still limited, the separation and purification process is also complicated, and the production cost is high, which will limit the popularization of more people in the market. Consumption

Method used

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Embodiment 1

[0047] A preparation method of high-purity mannooligosaccharides, comprising the steps of:

[0048] (1) Manno-oligosaccharide mixture produced by enzymatic hydrolysis and transformation: use Aspergillus niger to ferment to produce highly active neutral β-mannanase, the preservation number is: CCTCC NO.M2012554, the enzyme activity is as high as 5000-8000μmol / ml The solution is purified and stored at 0 or 1 or 2 or 3 or 4 or 5°C for use. Add 2 tons of water to a 5-ton enzymatic hydrolysis tank, heat it to 55°C with steam, add 4L of neutral β-mannanase enzyme solution, stir for 2 or 3 minutes to activate the enzyme solution, and slowly add the viscosity to 20000mpa.s Konjac fine powder 400kg, add in 1 hour. Keep the enzymolysis temperature at 53 or 54 or 55 or 56 or 57°C, pH6.5, enzymolysis time or 2 or 3 or 4 hours, stop enzymolysis when the viscosity of the mannan-oligosaccharide mixture is 40mpa.s, and obtain the oligosaccharide mixture , wherein the biotransformation rate ...

Embodiment 2

[0063] A preparation method of high-purity mannooligosaccharides, comprising the steps of:

[0064] (1) Manno-oligosaccharide mixture produced by enzymatic hydrolysis and conversion: using Aspergillus niger to ferment to produce a highly active neutral β-mannanase, the preservation number is: CCTCC NO.M2012554, the enzyme activity is as high as 4500 μmol / ml, and the enzyme solution is passed through The purification process is stored at 0-5°C for later use. Add 2.5 tons of water into a 5-ton enzymatic hydrolysis tank, heat it to 54°C with steam, add 4.5L of neutral β-mannanase enzyme solution, stir for 2 to 3 minutes to activate the enzyme solution, and slowly add the viscosity to 20000mpa. s konjac powder 550kg, add in 1 hour. Keep the enzymolysis temperature at 53-56°C, pH6.5, enzymolysis time 3.5 hours, measure the viscosity of the manno-oligosaccharide mixture to 60mpa.s, stop the enzymolysis, and obtain the oligosaccharide mixture, in which the glucomannan in konjac flou...

Embodiment 3

[0080] A preparation method of high-purity mannooligosaccharides, comprising the steps of:

[0081] (1) Manno-oligosaccharide mixture produced by enzymatic hydrolysis and conversion: using Aspergillus niger to ferment to produce a highly active neutral β-mannanase, the preservation number is: CCTCC NO.M2012554, the enzyme activity is as high as 4500 μmol / ml, and the enzyme solution is passed through The purification process is stored at 0-5°C for later use. Add 1.5 tons of water into a 5-ton enzymatic hydrolysis tank, heat it to 53°C with steam, add 2.5L of neutral β-mannanase enzyme solution, stir for 3 minutes to activate the enzyme solution, and slowly add Guar gum 255kg, added in 1 hour. Keep the enzymolysis temperature at 53-57°C, pH 6.0, and enzymolysis time for 4.5 hours to measure the viscosity of the mannan-oligosaccharide mixture to 70mpa. The biotransformation rate is 96%.

[0082] (2) Centrifugal separation:

[0083] Use a horizontal screw centrifuge LW250-1000 t...

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Abstract

The invention discloses a preparation method of high-purity mannan oligosaccharide. The preparation method comprises the steps of 1, producing a mannan oligosaccharide mixture through enzymolysis conversion; 2, carrying out centrifugal separation: separating enzymatic hydrolysate through a centrifugal machine; 3, carrying out ceramic membrane filtration: filtering centrifugate through a ceramic membrane group as precision separation to remove protein, starch and polysaccharide substances; 4, carrying out ion exchange refining: desalting, decoloring and deodorizing filtrate through a mannan oligosaccharide ceramic membrane; 5, carrying out 2000 molecular weight ultrafiltration: ultrafiltering ion exchange refining liquid through a polyether sulfone-polyamide composite membrane with interception as Dalton to obtain a polymerization degree; 6, carrying out nanofiltration: filtering sugar liquid through a ceramic membrane, ion exchange refining and an ultrafiltration membrane; and 7, carrying out spray drying: preheating a nano-filtered concentrated solution, and drying in a spray drying tower. The dried product contains more than 95% of mannan oligosaccharide, and has an obvious function of multiplying bifidobacterium; meanwhile, the product, as a most promising substitute, can reduce harmful bacteria.

Description

Technical field [0001] The present invention involves the technical field of konjac mannite hypotonal production, and it also involves a method of preparation of mannite hypotonal, especially the method of separating high -purity glycotose with ceramic membrane separation.Konjac one -dew hypotonal can be used for food additives, which are suitable for dairy products, biscuits, acidic beverages, etc.It can also be made into a health product powder and oral liquid preparation. It has enhanced immunity function, auxiliary blood lipids, auxiliary blood sugar -lowering functions, regulating intestinal flora functions, and laxative functions. It plays a good health effect on people. Background technique [0002] Portal hydrolycean (oligosaccharides, Oligosaccharides) refers to a type of sugar that is connected by 2 to 10 liquids to form a direct or branch chain through the glucoside bond.In addition to the good physical and chemical characteristics such as low thermal value, stability,...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C12P19/00C07H3/06C07H1/06C08B37/00
Inventor 王光荣赵西选杨富键向阳梅德斌徐曙光余梦成
Owner ENSHI TINJOY BIO TECH
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