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A kind of preparation method of compound microecological preparation effervescent tablet

A technology of compound microecology and effervescent tablets, which is applied in the direction of pharmaceutical formulations, drug combinations, and medical preparations containing active ingredients, etc. It can solve the inconvenience of storage and transportation for users, it is difficult to ensure accurate measurement of animals, and reduce the number of viable bacteria To achieve the effect of improving feed utilization, increasing convenience and reducing stress

Active Publication Date: 2016-06-22
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this feeding method is simple and convenient, since the probiotics are mostly lactic acid bacteria, their vitality is relatively fragile, and mixing them in feed will significantly reduce the number of viable bacteria
In addition, due to co-feeding with the feed, it is difficult to ensure the accurate measurement of animal absorption, thus affecting the curative effect
In addition, the freeze-dried product must be kept in a "cold chain" environment at a lower temperature during the entire storage and transportation process, which brings a lot of inconvenience to the storage and transportation of the product and the use of users.

Method used

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  • A kind of preparation method of compound microecological preparation effervescent tablet
  • A kind of preparation method of compound microecological preparation effervescent tablet
  • A kind of preparation method of compound microecological preparation effervescent tablet

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of probiotics

[0034] (1) Preparation of seeds for production

[0035] Culture of lactic acid bacteria

[0036] Preheat the MRS liquid medium to 37°C, inoculate the lactic acid bacteria seed liquid at 2% inoculation amount, and culture it at 37°C for 18-24 hours, harvest the bacterial liquid and store it at -4°C for later use.

[0037] Culture of Bacillus

[0038] Preheat the Bacillus culture medium to 37°C, inoculate the Bacillus seed solution with 1% inoculation amount, and culture it at 37°C for 18-24 hours, harvest the bacteria solution and store it at 4°C for later use.

[0039] The lactic acid bacteria are: one or more of Bifidobacterium bifidum, Lactobacillus lactis, Lactobacillus buchneri, and Bifidobacterium longum.

[0040] The bacillus is: one or more of Bacillus licheniformis, Bacillus coagulans and Bacillus subtilis.

[0041] Described MRS liquid culture medium is:

[0042] Peptone 10.0g, beef extract 10.0g, yeast extract...

Embodiment 2

[0045] Embodiment 2: Preparation of compound Chinese medicine polysaccharide and Chinese medicine flavone

[0046] a. According to 20 parts of Salvia miltiorrhiza, 15 parts of licorice, 20 parts of ginkgo biloba, 20 parts of astragalus, 15 parts of ginseng, 20 parts of Codonopsis pilosula, and 20 parts of wolfberry, weigh each Chinese medicine raw material, chop it, wash it, soak it in cold water overnight, and then add Purified water with 15 times the weight of the raw material, in a water bath to 90°C, and kept at 90°C, boiled for 2 hours, stirring every 10 minutes during the boiling process;

[0047] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0048] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, and the supernatan...

Embodiment 3

[0054] Embodiment 3: preparation of lyoprotectant

[0055] Each 1000ml phosphate buffer solution contains the following components: 40g of Chinese medicine polysaccharides, 30g of Chinese medicine flavonoids, 8g of vitamin combination (vitamin C1.6g, vitamin E0.8g, vitamin D30.6g, vitamin B12.2g, vitamin K1.6g, vitamin B21. 2g), gelatin 5g, sucrose 50g; or every 1000ml of phosphate buffer contains the following components: Chinese medicine polysaccharide 70g, Chinese medicine flavonoids 40g, vitamin combination 7g (vitamin C1.4g, vitamin E1.75g, vitamin D30.7g, vitamin B11. 4g, vitamin K0.7g, vitamin B21.05g), gelatin 6g, sucrose 60g. Described Chinese medicine polysaccharide and Chinese medicine flavonoid are obtained by embodiment 2.

[0056] Preparation method: Accurately weigh each component, dissolve in 1000ml of phosphate buffer solution, filter and sterilize through a 0.22um filter membrane under sterile conditions, and store at 4°C for later use.

[0057] Phosphate b...

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Abstract

The invention discloses a method for preparing effervescent tablets of compound probiotics, which belongs to the technical field of veterinary biological products. It includes the following process steps: 1) preparation of probiotics; 2) freeze-drying of mixed preparations; 3) preparation of effervescent tablets. The present invention has the following beneficial effects: the compound probiotic effervescent tablet prepared by the present invention reduces the transportation requirements of the product and increases the convenience for users to use; the compound probiotic effervescent tablet prepared by the present invention can be used in drinking water The feeding of probiotics while immunizing live vaccines reduces manpower and material resources, and reduces the stress on chickens; the compound probiotic effervescent tablets prepared by the present invention can enhance the immunity of chickens and improve the feed quality of chickens. Utilization rate, increase the egg production rate of chickens, reduce ammonia emissions from chicken houses, and improve the living environment of chickens.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a compound microecological preparation effervescent tablet. Background technique [0002] Probiotics have beneficial effects on humans and animals. Probiotics in probiotics can produce antibacterial substances, compete with harmful bacteria for niches, inhibit the growth and reproduction of pathogenic bacteria, and adjust and restore the balance of intestinal flora. In addition, probiotics can become non-specific immune factors, stimulate host cells through the bacteria themselves or cell wall components, activate the immune system, increase the body's antibody level or macrophage activity, thereby enhancing the body's resistance. The beneficial bacteria in the probiotics multiply in the digestive tract, which can promote the effective synthesis, absorption and utilization of a series of nutrients such as amino acids and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/46A61K36/815A61K35/744A61K35/742A61P37/04A61K31/715
Inventor 张秀文李阳闫艳丽刘雪王二先盛璐丝
Owner 浙江美保龙生物技术有限公司
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