Preparation process of live bacillus mucilaginosus preparation

Abstract

The invention relates to a preparation process of a live bacillus mucilaginosus preparation. The preparation process comprises the steps that bacillus mucilaginosus is inoculated into a fermentation medium to be cultured after being subjected to primary and secondary seed enlarged culture; the quantity and conversion rate of bacillus are increased through fed-batch of a carbon source in the fermentation process, thus obviously increasing the unit yield and reducing the unit cost; after fermentation is completed, the fermentation liquor is concentrated by using a vacuum film concentrator and the soluble synergistic substances, growth-stimulated substances and bacteriostatic active substances in the fermentation liquor can be effectively recovered; the concentrated fermentation liquor is dried and granulated by multistage low-temperature spray fluidized drying equipment, the granules are sorted and the fine powder is trapped by a cyclone separator and returns to be re-granulated, thus obtaining the bacillus mucilaginosus product, each gram of the powder of which has not less than 2.0*10<10> of CFU (colony-forming units); the tail gas is treated and is discharged up to the standard. The preparation process has the advantages that the active substances in the fermentation liquor can be furthest retained; the product quality and the equipment utilization rate are high; the biological activity is better maintained; the waste gas and the wastewater are discharged up to the standard; the preparation process can be used for large-scale industrial production.

Description

technical field [0001] The invention relates to a preparation process of a jelly-like bacillus living bacteria preparation. Background technique [0002] Bacillus mucilaginosus, commonly known as potassium bacteria, is a common soil bacterium in farmland. In the early 1950s, the Soviet scholar Aleksandrov first reported that this special bacterium was isolated from the soil, which not only has the ability to activate potassium in silicate minerals, but also can use phosphorus in apatite and fix atmospheric nitrogen. , so called silicate bacteria. The shape of the bacteria is thick and long rod, the end is round, the size is 1-1.2×4-7 microns, it does not move, the Gram staining is indeterminate, and there are often 1-2 large fat particles in the bacteria. In the nutrient-poor selective medium, the bacterium is covered with an oval hypertrophic capsule, the size of which is 5-7×7-10 microns, which is 10-20 times or even 50 times larger than the bacterium; There are even 2-...

AI Technical Summary

Problems solved by technology

[0007] 1. The filling coefficient of the fermentation tank is 60%-70%, and the utilization rate of the equipment is not high;

[0008] 2. Due to the batch fermentation used, the initial concentration of the fermentation medium is too high, the lag period after inoculation is longer, and more by-products are formed during the fermentation process, and the concentration of the fermentation product cannot increase, which eventually leads to higher fermentation costs;

[0009] 3. Flocculation / plate-and-frame filtration or centrifugation technology is generally used for the concentration of fermentation broth. These two technologies generally generate a large amount of wastewater, and the subsequent treatment costs are relatively high;

[0010] 4. The use of flocculation / plate-and-frame filtration or centrifugation technology will also cause the loss of active ingredients in the fermentation supernatant, resulting in a decline in the product effect;

[0011] 5. The inlet temperature of spray drying is generally set at 180°C to 200°C, and the outlet temperature is 80°C to 100°C. Under such a high working temperature, the number of bacteria in some varieties will decrease to a certain extent, which directly affects product quality

[0014] Fermentation and drying and granulation will produce a large amount of waste gas, which often contains volatile organic compounds (VOC), hydrogen sulfide, ammonia, mercaptans and other pollutants, accompanied by unpleasant stench. In the past, fermentation enterprises generally Discharge it directly or simply spray it with water, the two treatment methods will cause serious pollution to the environment