Flexible electrochemical electrode, continuous glucose monitoring sensor and preparation method thereof

Abstract

The invention discloses a flexible electrochemical electrode, a continuous glucose monitoring sensor equipped therewith and a preparation method thereof. Golden layers on two sides of a chemical gold-plating film are utilized as a working electrode and a comparison-counter electrode in order to form an electrochemistry electrode system. Petal-shaped platinum nanoparticles are subjected to electro-deposition on the surface of the working electrode as a catalysis layer. Then, electrophoretic deposition carbon nanotube / Nafion mesh layer is utilized as an anti-interference layer. An enzyme biochemical sensitive layer is formed through static adsorption on the anti-interference layer and coated with a polyurethane mass transfer restriction protection layer after cross-linking and solidity of glutaraldehyde. Therefore, the continuous glucose monitoring sensor is prepared. Without lithography or screen printing and other technologies, an electrochemical electrode system can be constructed so that processing technology is effectively simplified and large scale production is easily achieved and production cost is lowered. In the meantime, advantages such as broad linear range, high interference immunity, high response sensitivity and long term stability are obtained.

Description

technical field [0001] The invention relates to a biosensor, in particular to a flexible electrochemical electrode, a continuous glucose monitoring sensor equipped with the electrochemical electrode and a preparation method thereof, belonging to the technical field of an amperometric continuous glucose monitoring sensor. Background technique [0002] Diabetes is one of the common chronic diseases that seriously threaten human health. It is still incurable, but effective blood sugar management can greatly reduce the incidence of complications and improve the quality of life of patients. Blood glucose testing is the gold standard for diabetes diagnosis and management. In order to solve the inconvenience and pain caused by fingertip puncture blood collection many times a day, industry technicians have created a continuous blood glucose monitoring system (CGMS) that can be implanted under the skin. Application No. PCT / US2005 / 032102 and International Publication No. WO / 2006 / 02929...

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Problems solved by technology

However, most of these methods are complicated in process and low in scale, which makes the production cost of such products high and expensive, which restricts the popularization and application of CGMS system

Moreover, for the sensor preparation process based on a flexible polymer film, the deposition of wires and electrodes on the insulating polymer film is usually achieved by vacuum magnetron sputtering, photolithography, screen printing, jet printing and other technologies, but vacuum The vapor-deposited metal layer often has poor bonding force with the substrate and is very easy to fall off; while the electroless plating method in the photolithography process, the method of electroless plating on the surface of the film usually contains dangerous or toxic reagents. With the ROHS and WEEE standards for this Strict control of chemical reagents, the strong oxidant surface etching process necessary in this type of process has gradually changed to low-temperature plasma surface treatment and transition layer process, but there are still complex processes, special requirements for the size and shape of the workpiece, and the combination of the coating and the substrate problems such as poor precision; screen printing materials have traditional techniques and mature technology, but there are also problems such as low precision, difficulty in miniaturization, and serious waste of materials. Now it has gradually developed into jet printing manufacturing, but this emerging method is still Faced with many challenges, such as the characteristic control of jet printing ink, nozzle clogging, the bonding force between the substrate and the ink, the curing method of the ink, etc.

In addition, for the process of using extremely thin metal wires as electrodes, it is generally realized by using expensive platinum-iridium filaments with polytetrafluoroethylene insulating jackets, and the wire part with the insulation layer removed is used as the working electrode, and the Ag / AgCl coated The silver paste or the part wound with thin Ag wire and chlorinated is used as the contrast electrode to realize the two-electrode electrochemical system. The raw material cost of this kind of process is high, and because the two-electrode system can only be configured in the radial direction, the electrode sensing part is relatively long , needs to be implanted in a deep part of the skin, which is prone to accidental damage to capillaries. Moreover, the manufacturing process of this sensor is complicated, which is not conducive to large-scale production, resulting in high production costs.

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