Silk fibroin hemostatic material and preparation method thereof

Abstract

The invention relates to the field of application of silk fibroin and especially relates to a silk fibroin hemostatic material and a preparation method thereof. The preparation method includes the following steps: S1) preparing a silk fibroin solution with mass percentage being 1-6%; S2) preparing a polyethylene glycol solution with mass percentage being 10-40%, wherein molecular weight of the polyethylene glycol is 1,000-10,000; S3) mixing the silk fibroin solution and the polyethylene glycol solution according to the volume ratio of 10:0.25-10:3, and performing freeze drying to prepare the silk fibroin hemostatic material. The silk fibroin hemostatic material has good water absorption, is tightly combined with a wound and is not liable to fall off, has excellent hemostatic effect on body surface and in-vivo hemorrhage, is degradable and absorbable and can prevent tissue adhesion due to operation, has no inflammatory reactions and is low in cost.

Description

technical field [0001] The invention relates to the application field of silk fibroin, in particular to a silk fibroin hemostatic material and a preparation method thereof. Background technique [0002] Conventional methods of hemostasis include manual extrusion, cauterization and wound suturing, but these methods sometimes cannot effectively stop bleeding, thereby prolonging the operation time. In addition, imperfect hemostasis methods often require secondary operations. For many years, in the bleeding treatment of skin wounds and soft tissue organ injuries such as spleen and kidney, most of them use naturally derived degradable hemostatic materials, mainly including gelatin sponge, oxidized cellulose, microfiber collagen powder, fibrin glue, and chitosan , collagen sponge, etc., and they each have certain shortcomings: 1) gelatin and collagen have poor tissue adsorption, and the hemostatic function of both depends on sufficient platelets and coagulation factors, so the app...

AI Technical Summary

Problems solved by technology

[0002] Conventional methods of hemostasis include artificial extrusion, cauterization and wound suturing, but these methods sometimes cannot effectively stop bleeding, thereby prolonging the operation time. In addition, incomplete hemostasis methods often require secondary operations

For many years, in the bleeding treatment of skin wounds and soft tissue organ injuries such as spleen and kidney, most of them use naturally derived degradable hemostatic materials, mainly including gelatin sponge, oxidized cellulose, microfiber collagen powder, fibrin glue, and chitosan , collagen sponge, etc., and they each have certain shortcomings: 1) gelatin and collagen have poor tissue adsorption, and the hemostatic function of both depends on sufficient platelets and coagulation factors, so the application is limited; 2) ) Fibrin glue comes from blood, which may cause virus infection; 3) Chitosan materials generally have poor solubility and low mechanical strength, and generally need to be dissolved with acid during processing, and it is easy to produce side effects on the human body after adding acid. Tissue hyperemia, redness, tears and other reactions; 4) The traditional cotton gauze treatment of skin wounds not only has a long healing period and obvious wound scars, but also the patient is quite painful during the dressing change process

However, these materials have the following disadvantages: 1) silk fibroin peptides are generally decomposed into small molecular peptide chains by technical means such as enzymatic hydrolysis, alkali hydrolysis, hydrolysis, and acid hydrolysis, which have low molecular weight and poor mechanical properties. mass production

2) Modified silk is processed by physical graft polymerization, X-ray radiation or organic solvents such as methanol, etc. to change the structure and characteristics of silk, such as the invention patent of CN102505453A, which discloses a modified However, the water absorption of modified silk is poor, which cannot fully combine silk fibroin with blood, which limits the hemostatic effect, and the operation process is complicated and the cost is high, so it is difficult to industrialize production

3) Silk fibroin composite materials, such as the combination of silk fibroin and chitosan, the combination of silk fibroin and hydroxyapatite, etc., such composite materials are not more effective in hemostasis than a single type of material, but instead increase the cost, the gain outweighs the gain

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