A yeast product with anti-oxidation and selenium supplement functions and preparation method thereof
A technology for anti-oxidation and selenium supplementation, which is applied in microorganism-based methods, biochemical equipment and methods, and microbial dissolution, etc., can solve the problems of only paying attention to the absorption and utilization rate of selenium, the absorption rate is not high, and the antioxidant function is ignored. Achieving the effect of helping digestion and absorption and promoting absorption
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[0025] The preparation method of yeast product in the embodiment of the present invention 1-9 is:
[0026] Add 20 μg / ml sodium selenite to the basal medium in six times at 4h, 7h, 10h in the logarithmic phase of growth of brewer’s yeast and 12h, 14h, and 16h in the stable growth phase, at a temperature of 30°C, shake the flask for 24h, and collect Bacteria liquid, centrifuged and dried to obtain bacteria, the selenium content of the obtained bacteria is 4000mg / kg; control humidity 80%, inactivate at 55°C for 2 hours, to ensure the death of yeast and keep the activity of intracellular enzymes; enter the enzymolysis tank, enzymolysis Before the tank is used, pre-exclude oxygen, fill with a certain volume of nitrogen, take the inactivated bacteria as the benchmark, add 0.5w% neutral protease, 0.3w% papaya enzyme, 0.3w% NaCl, adjust to pH = 6.0, temperature 55 Autolyze at ℃ for 20 hours; drop to 4℃ and immediately collect from the solution for centrifugation at a centrifugation sp...
Embodiment 1
[0028] The yeast product was added to the base material at a dosage of 0.2 mg / kg, and the feed was delivered on 20 days.
[0029] The ingredients of the above-mentioned base material are: 45% of corn, 15% of soybean meal, 10% of rapeseed meal, 10% of rice bran meal, 15% of wheat bran, 4% of premixed feed, and 1% of soybean oil.
[0030] The formula of the above premixed feed is: vitamin premix 25‰, trace element premix 125‰, lysine 50‰, choline 30‰, pig compound enzyme 35‰, calcium 4.5‰, phosphorus 1.5‰, salt 3 ‰, flavoring agent 36‰, stone powder 200‰, calcium hydrogen phosphate 240‰, carrier 250‰.
[0031] The active ingredients of the above vitamin premix are: vitamin A6750IU / kg, vitamin D1500IU / kg, vitamin E30mg / kg, vitamin K1.5mg / kg, vitamin B 1 1.5mg / kg, vitamin B 2 3.8mg / kg, vitamin B 6 2.3mg / kg, vitamin B 12 17μg / kg, niacin 15mg / kg, calcium pantothenate 7.5mg / kg, folic acid 0.8mg / kg, biotin 0.12mg / kg.
[0032] The active ingredients of the above trace element ...
Embodiment 2
[0034] The difference from Example 1 is that the animals were fed within 30 days.
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