Application of Japanese ginseng polysaccharide to preparation of medicine for improving immunologic function of patients suffering from liver cancer
A technology of bamboo ginseng polysaccharide and immune function, which is applied in the application field of medicine to achieve high purity, good dose dependence, and the effect of increasing thymus/spleen index
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Embodiment 1
[0025] Preparation of bamboo ginseng polysaccharide:
[0026] The raw material of bamboo ginseng is the dried rhizome of Panax japonicus C.A.Mey., a plant of the Araliaceae genus Panax japonicus.
[0027] Proceed as follows:
[0028] Take the dried bamboo ginseng medicinal powder, add 95% ethanol (V / V, the percentage concentration of ethanol mentioned in this manual refers to the volume percentage concentration, and will not be described later), reflux at 60 ° C for 3 hours, and degrease;
[0029] Then add 80% ethanol, reflux at 60°C for 2 hours, remove monosaccharides and oligosaccharides;
[0030] After drying the dregs in an oven, use purified water as a solvent to reflux extract 3 times, 2 hours each time, combine the filtrate and concentrate it in a rotary evaporator until it is in the state of liquid extract, add ethanol, and adjust the ethanol concentration to 80 %, stay overnight at 4°C, filter the next day, precipitate and dry to obtain the crude bamboo ginseng;
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Embodiment 2
[0040] Effect of the bamboo ginseng polysaccharide prepared in Example 1 on the immune function of liver cancer H22 tumor-bearing mice
[0041] 2.1 Experimental materials
[0042] 2.1.1 Animals and cell lines
[0043] Kunming mice: half male and half male, weighing 18-22 g, provided by the Hubei Provincial Center for Disease Control and Prevention, license number: SCXK (Hubei): 2008-0003.
[0044] The H22 cell line was donated by Mr. Chen Luyi, School of Pharmacy, South Central University for Nationalities, and stored in a -80°C refrigerator. Centrifuge during recovery, remove DMSO in the freezing solution, dilute with normal saline to 1*10 6 1 / mL, inoculate 0.2mL of each mouse in the abdominal cavity, pass passage after seven days, take the ascites of the third-generation H22 ascites tumor mouse, dilute it with normal saline at 1:10, and inoculate 0.2mL of each mouse on the right forelimb of the mouse Under the skin of the axilla, the H22 solid tumor model was established....
Embodiment 3
[0101] Protective effect of the bamboo ginseng polysaccharide prepared in Example 1 on LPS / D-GalN-induced acute liver injury in mice:
[0102] LPS / D-GalN induced acute liver injury in mice was used as a model to study the preventive and protective effects of bamboo ginseng polysaccharides on acute liver injury and try to explore its mechanism.
[0103] 3.1 Experimental animals and reagents
[0104] 3.1.1 Experimental animals
[0105] 48 male Kunming mice of SPF grade, weighing 18-22 g, were purchased from Hubei Experimental Animal Research Center, experimental animal production license number: SCXK (Hubei) 2015-0018.
[0106] 3.1.2 Reagents
[0107] Panax ginseng polysaccharide: prepared in Example 1; lipopolysaccharide (LPS) was purchased from Biosharp; galactosamine (D-GalN) was purchased from Tokyo Chemical Industry; alanine aminotransferase (ALT), aspartic acid amino Transferase (AST), reduced glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) assay...
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