Application of Japanese ginseng polysaccharide to preparation of medicine for improving immunologic function of patients suffering from liver cancer

A technology of bamboo ginseng polysaccharide and immune function, which is applied in the application field of medicine to achieve high purity, good dose dependence, and the effect of increasing thymus/spleen index

Inactive Publication Date: 2018-04-20
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Have not yet seen related reports in the prior art, this application will fill up this gap

Method used

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  • Application of Japanese ginseng polysaccharide to preparation of medicine for improving immunologic function of patients suffering from liver cancer
  • Application of Japanese ginseng polysaccharide to preparation of medicine for improving immunologic function of patients suffering from liver cancer
  • Application of Japanese ginseng polysaccharide to preparation of medicine for improving immunologic function of patients suffering from liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of bamboo ginseng polysaccharide:

[0026] The raw material of bamboo ginseng is the dried rhizome of Panax japonicus C.A.Mey., a plant of the Araliaceae genus Panax japonicus.

[0027] Proceed as follows:

[0028] Take the dried bamboo ginseng medicinal powder, add 95% ethanol (V / V, the percentage concentration of ethanol mentioned in this manual refers to the volume percentage concentration, and will not be described later), reflux at 60 ° C for 3 hours, and degrease;

[0029] Then add 80% ethanol, reflux at 60°C for 2 hours, remove monosaccharides and oligosaccharides;

[0030] After drying the dregs in an oven, use purified water as a solvent to reflux extract 3 times, 2 hours each time, combine the filtrate and concentrate it in a rotary evaporator until it is in the state of liquid extract, add ethanol, and adjust the ethanol concentration to 80 %, stay overnight at 4°C, filter the next day, precipitate and dry to obtain the crude bamboo ginseng;

...

Embodiment 2

[0040] Effect of the bamboo ginseng polysaccharide prepared in Example 1 on the immune function of liver cancer H22 tumor-bearing mice

[0041] 2.1 Experimental materials

[0042] 2.1.1 Animals and cell lines

[0043] Kunming mice: half male and half male, weighing 18-22 g, provided by the Hubei Provincial Center for Disease Control and Prevention, license number: SCXK (Hubei): 2008-0003.

[0044] The H22 cell line was donated by Mr. Chen Luyi, School of Pharmacy, South Central University for Nationalities, and stored in a -80°C refrigerator. Centrifuge during recovery, remove DMSO in the freezing solution, dilute with normal saline to 1*10 6 1 / mL, inoculate 0.2mL of each mouse in the abdominal cavity, pass passage after seven days, take the ascites of the third-generation H22 ascites tumor mouse, dilute it with normal saline at 1:10, and inoculate 0.2mL of each mouse on the right forelimb of the mouse Under the skin of the axilla, the H22 solid tumor model was established....

Embodiment 3

[0101] Protective effect of the bamboo ginseng polysaccharide prepared in Example 1 on LPS / D-GalN-induced acute liver injury in mice:

[0102] LPS / D-GalN induced acute liver injury in mice was used as a model to study the preventive and protective effects of bamboo ginseng polysaccharides on acute liver injury and try to explore its mechanism.

[0103] 3.1 Experimental animals and reagents

[0104] 3.1.1 Experimental animals

[0105] 48 male Kunming mice of SPF grade, weighing 18-22 g, were purchased from Hubei Experimental Animal Research Center, experimental animal production license number: SCXK (Hubei) 2015-0018.

[0106] 3.1.2 Reagents

[0107] Panax ginseng polysaccharide: prepared in Example 1; lipopolysaccharide (LPS) was purchased from Biosharp; galactosamine (D-GalN) was purchased from Tokyo Chemical Industry; alanine aminotransferase (ALT), aspartic acid amino Transferase (AST), reduced glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) assay...

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Abstract

The invention belongs to the technical field of medicine application of plant polysaccharide and discloses the application of Japanese ginseng polysaccharide to the preparation of a medicine for improving immunologic function of patients suffering from liver cancer. The invention discovers for the first time that the Japanese ginseng polysaccharide can obviously inhibit in-vivo growth of H22 livercancer cells under a certain dosage, and the Japanese ginseng polysaccharide obviously increases the proportion of CD8+T and NK cells by increasing thymus/spleen index of the organism and spleen cellproliferation and enhances the cytotoxic activity on the liver cancer H22 tumor cells. The Japanese ginseng polysaccharide can stimulate immune response of the mouse organism to exert the anti-tumoreffect, and the toxicity on the tumor-bearing mouse is not detected. The related action mechanism comprises the followings: the Japanese ginseng polysaccharide can improve the ability of tumor transplantation induced CD4+T cell apoptosis; the immunostimulation factor level in the tumor-bearing mouse is increased and the immunosuppression factor level is reduced; the tumor-bearing mouse CD4+T cellsare promoted to be differentiated into Th1 to take part in anti-tumor immunostimulation activity; and the immunostimulation activity is generated by resisting inflammation and adjusting polarizationof TAMs, so that the anti-tumor effect is achieved.

Description

technical field [0001] The invention relates to the technical field of medical application of plant polysaccharides, in particular to the application of bamboo ginseng polysaccharides in the preparation of drugs for improving the immune function of patients with liver cancer. Background technique [0002] Liver cancer is a kind of malignant tumor that seriously endangers human health, and its morbidity and mortality have remained high in recent years. The pathogenesis of liver cancer is very complex, among which chronic persistent inflammation and loss of tumor suppressor genes are important factors leading to the occurrence of liver cancer. At present, the mechanisms of most tumor suppressor genes regulating the proliferation and apoptosis of liver cancer cells have been studied clearly. In view of the fact that some tumor suppressor genes are also expressed in immune cells, they have dual functions of inhibiting inflammation and cancer in both immune cells and tumor cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P37/04A61P35/00
CPCA61K31/715
Inventor 邓旭坤江善青舒广文
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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