Method for extracting antimicrobial peptide from chicken blood
A technology of antimicrobial peptide and chicken blood, applied in the field of biological extraction, can solve the problems of low enzymatic hydrolysis efficiency, achieve the effect of improving enzymatic hydrolysis degree, low investment, and inhibiting thrombin activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] The method of extracting bacteriostatic peptides from chicken blood is to treat fresh chicken blood with a sodium hexametaphosphate aqueous solution containing fatty acid methyl ester and triethylamine for anticoagulation, then prepare a fermentation medium, inoculate Aspergillus niger to ferment the fermentation broth, and ferment The solution is subjected to ultrafiltration and gel column chromatography to obtain chicken blood bacteriostatic peptide. Aspergillus niger has a powerful polymer degrading enzyme system, which can grow and ferment quickly on a variety of cheap media. During the process of growth and reproduction, various metabolic enzymes, such as acid protease and neutral protease, are produced, which can be effective The degradation of macromolecular proteins into small molecular peptides has the advantages of low investment, simple operation, good protein degradation effect, and yield of antimicrobial peptides.
[0020] The method of extracting bacteriostat...
Embodiment 2
[0029] The method of extracting bacteriostatic peptide from chicken blood, its specific steps are:
[0030] 1) Anticoagulant treatment of chicken blood: add a 3% sodium hexametaphosphate aqueous solution to fresh chicken blood at a volume ratio of 1:5, stir evenly, and then centrifuge at a temperature of 4℃ and a speed of 5000r / min Centrifuge in the machine for 30 minutes, discard the precipitate, and freeze-dry the supernatant to obtain anticoagulated chicken blood meal for use;
[0031] 2) Preparation of fermentation medium: take 8 parts by weight of anticoagulant chicken blood meal, 20 parts of sucrose, 5 parts of sodium chloride, 0.005 parts of zinc chloride, 2.0 parts of potassium dihydrogen phosphate, 0.4 parts of magnesium sulfate, and 0.01 parts of iron sulfate Vitamin B 1 0.001 parts, 1000 parts of sterile water, mix evenly to obtain fermentation medium;
[0032] 3) Aspergillus niger fermentation: adjust the pH of the fermentation medium to 6.0, then inoculate Aspergillus n...
Embodiment 3
[0038] The method of extracting bacteriostatic peptide from chicken blood, its specific steps are:
[0039] 1) Anticoagulant treatment of chicken blood: add a 3% sodium hexametaphosphate aqueous solution to fresh chicken blood at a volume ratio of 1:5, stir evenly, and then centrifuge at a temperature of 4℃ and a speed of 5000r / min Centrifuge in the machine for 30 minutes, discard the precipitate, and freeze-dry the supernatant to obtain anticoagulated chicken blood meal for use;
[0040] 2) Preparation of fermentation medium: take 8 parts by weight of anticoagulant chicken blood meal, 20 parts of sucrose, 5 parts of sodium chloride, 0.005 parts of zinc chloride, 2.0 parts of potassium dihydrogen phosphate, 0.4 parts of magnesium sulfate, and 0.01 parts of iron sulfate Vitamin B 1 0.001 parts, 1000 parts of sterile water, mix evenly to obtain fermentation medium;
[0041] 3) Aspergillus niger fermentation: adjust the pH of the fermentation medium to 6.0, then inoculate Aspergillus n...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com