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A method for preparing sericin film material based on enzymatic graft copolymerization

A technology of membrane material and sericin, which is applied in the field of textile chemistry, can solve the problems of decreased biocompatibility of sericin material and harsh reaction conditions, etc., and achieves the reduction of sericin water solubility, high graft copolymerization efficiency, and mild enzyme treatment conditions Effect

Active Publication Date: 2020-01-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical cross-linking method has high efficiency and can improve the formability of sericin materials, but there are also some shortcomings, such as relatively harsh reaction conditions, and some cross-linking agent residues will reduce the biocompatibility of sericin materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Prepare the sericin film material with the method mentioned in the present invention, concrete process step is as follows:

[0043] (1) Preparation of vinyl diazonium salt: Dissolve 10 g / L 3-vinylaniline with 5 g / L hydrochloric acid, add 7.5 g / L sodium nitrite to prepare diazonium salt solution, and treat at 0°C for 40 minutes; After the reaction, use starch-potassium iodide test paper to detect nitrous acid in the diazonium salt solution, remove excess nitrous acid with urea, and adjust the pH of the diazonium salt solution to 9.0;

[0044] (2) Coupling reaction of sericin: add sericin derived from mulberry silk to the diazonium salt solution prepared in step (1), and perform a coupling reaction at 0°C for 10 minutes, wherein the sericin is 10 g / L; then the sericin solution Dialysis against deionized water for 24 hours;

[0045](3) Graft copolymerization of enzymatic sericin and monomer: add methyl methacrylate, horseradish peroxidase and acetylacetone to the sericin ...

Embodiment 2

[0052] Prepare the sericin film material with the method mentioned in the present invention, concrete process step is as follows:

[0053] (1) Preparation of vinyl diazonium salt: Dissolve 12 g / L 4-vinyl aniline with 7.5 g / L hydrochloric acid, add 8 g / L sodium nitrite to prepare diazonium salt solution, and treat at 0°C for 50 min After the reaction finishes, detect nitrous acid in the diazonium salt solution with starch-potassium iodide test paper, remove excessive nitrous acid with urea, and adjust the pH of the diazonium salt solution to 8.5;

[0054] (2) Coupling reaction of sericin: add sericin derived from mulberry silk to the diazonium salt solution prepared in step (1), and perform a coupling reaction at 0°C for 15 minutes, wherein the sericin is 12 g / L; then the sericin solution Dialysis against deionized water for 20 hours;

[0055] (3) Enzyme-catalyzed graft copolymerization of sericin and monomers: add methyl acrylate, horseradish peroxidase and acetylacetone to t...

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Abstract

The invention discloses a method for preparing sericin film material based on enzymatic graft copolymerization, and belongs to the technical field of textile chemistry. According to the method, through coupling reaction and catalyzing, graft copolymerization is conducted on a vinyl ester monomer, so that the water solubility of sericin protein is reduced, and the forming effect of the sericin filmmaterial is improved; through diazotization of vinyl-containing arylamine, diazonium salt is prepared, then the diazonium salt and the sericin protein are subjected to coupling reaction, and finally,enzymatic graft copolymerization is conducted on sericin and the vinyl ester monomer to prepare the sericin film material. The method comprises the following processes and steps of (1) preparation ofthe vinyl-containing diazonium salt; (2) the sericin protein coupling reaction; (3) enzymatic graft copolymerization of the sericin and the monomer; (4) forming of the sericin film material. Comparedwith a method in which a chemical cross-linking method is adopted for introducing a vinyl monomer, and then persulfate or an ultraviolet radiation method and the like is used for triggering graft copolymerization of the sericin and the vinyl ester monomer, the method has high graft copolymerization efficiency, mild enzymatic treatment conditions, and improved formability of the sericin film material.

Description

technical field [0001] The invention relates to a method for preparing sericin membrane material based on enzymatic graft copolymerization, which belongs to the technical field of textile chemistry. Background technique [0002] Silk is composed of silk fibroin and sericin covering the surface of silk fibroin. Silk fibroin has a linear structure and high crystallinity, and is mostly used in textile fiber processing; sericin is a spherical protein, containing more hydrophilic polar amino acids, and its supramolecular structure is mainly random coils, with high water solubility. After the silk is degummed by alkaline method or protease method, the sericin in the outer layer of silk is dissolved in hot water, and most of them are discharged into industrial wastewater as waste, resulting in a great waste of resources and environmental pressure. [0003] There are many polar amino acids in sericin, including carboxyl-containing glutamic acid, glutamine and aspartic acid, phenoli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F289/00C08F220/14C08H1/00C08J5/18C08L51/00
CPCC08F289/00C08H1/00C08J5/18C08J2351/00C08F220/14
Inventor 王平胡浩然袁久刚王强范雪荣
Owner JIANGNAN UNIV
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