Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases

A technology of Bacillus velesi and Bacillus, applied in the application, chemicals and bacteria for biological control, etc., can solve the problems of imperfect bio-control bacteria resource pool and environmental pollution, and reduce the occurrence of banana diseases. , good control effect

Active Publication Date: 2018-12-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extensive use of chemical pesticides not only causes severe environmental pollution, but also makes patho

Method used

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  • Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases
  • Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases
  • Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1 Isolation and screening of Bacillus 3-10

[0037] 1. Strain isolation

[0038] Sample collection: In the vegetable field in Huadu District, Guangzhou City, Guangdong Province, soil samples less than 5 cm from the surface of the soil were dug and stored in bags, and then brought back to the laboratory for strain isolation.

[0039] Strain isolation: the soil samples are separated by the dilution coating plate method. Specifically, after the collected soil sample is thoroughly mixed, 4.0 g is weighed, soaked in 20 mL of sterile water, shaken, and incubated at 28° C. and 180 rpm on a shaker for 20 minutes to perform gradient dilution. Take the dilution factor as 10 5 -10 9 Spread 100 µL of the diluted solution on the LB plate, dry it at room temperature, seal it, and invert it in an incubator at 28°C for 24 h. Pick a single colony growing on the LB plate, label it, and place it in a 2 mL centrifuge tube containing 500 µL of liquid LB. Incubate at 28°C and shake on a 1...

Example Embodiment

[0041] Example 2 Identification of Bacillus 3-10

[0042] 1. Morphological identification

[0043] Strains 3-10 are Gram-positive; it can reduce nitrates, liquefy gelatin, produce acid and peptone, produce catalase, amylase, and can utilize fructose, trehalose, glucose and sucrose.

[0044] 2. Molecular identification

[0045] In order to clarify the taxonomic status of strains 3-10 obtained in Example 1, we used multilocus sequence analysis (MLSA) combined with phylogenetic analysis (Phylogenetic analysis) to identify strains 3-10.

[0046] 16S rDNA sequence analysis is a molecular biological identification method for bacterial identification. The present invention analyzes 16S rDNA sequences and combines conservative housekeeping genes. gyrA with gyrB Sequence, use MEGA 5.0 software and Neighbor-joining method to construct a phylogenetic tree, comprehensively analyze the evolutionary relationship of 3-10, the research shows that the 16S rDNA sequence of 3-10 is Bacillus velezensis...

Example Embodiment

[0049] Example 3 Determination of antibacterial spectrum of biocontrol bacteria 3-10

[0050] 1. In order to better study the biocontrol potential of the strain 3-10 of the present invention, we have studied the antibacterial spectrum of the strain. The specific operations are as follows:

[0051] (1) Explore whether 3-10 has the ability to inhibit the growth of other pathogenic bacteria. Using the same method as the strain screening in Example 1, replace the pathogenic bacteria MS2 with the same amount of rice basal rot fungus EC1, banana bacterial soft rot MS3, and bacterial wilt fungus EP1 with the same culture conditions and methods (first layer 15 mL Replaced LB with 15 mL TTC), cruciferous black rot fungus Xcc, rice bacterial blight fungus Xoo, peach rot fungus Pantoea ananatis PP1, yellow skin rot pathogen Pantoea anthophila CL1, the remaining steps remain unchanged.

[0052] (2) Explore whether 3-10 has the ability to inhibit the growth of pathogenic fungi. Fungi for tes...

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Abstract

The invention discloses a broad-spectrum antibacterial effect of Bacillus velezensis 3-10 and its application in prevention and treatment on banana diseases. The strain is preserved in the Guangdong provincial microbial culture collection center on April 30, 2018 and has a preservation number of GDMCC NO. 60366. The strain 3-10 has broad-spectrum antibacterial effects on banana bacterial soft rotpathogen, Ralstonia solanacearum, xanthomonas campestris, peach rot pathogen, Clausena lansium rot pathogen, colletotrichum capsici, colletotrichum gloeosporioides, banana fusarium wilt pathogen, rhizoctonia solani, Xanthomonas oryzae pv. oryzae, peronophythora litchi, magaporthe grisea, ustilago maydis and Xanthomonas campestris pv., can significantly alleviate the occurrence of banana bacterialsoft rot and banana wilt, and has better effects in a pot experiment. The strain can be used to develop a broad spectrum of biological pesticide for preventing and treating important crop bacterial and fungal diseases and especially for preventing and treating banana diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. More specifically, a strain of Bacillus Velez ( Bacillus velezensis ) 3-10 and its broad-spectrum antibacterial effect and its application in the control of banana diseases. Background technique [0002] Banana is one of the four major fruits in the world. my country's banana production areas are mainly concentrated in Yunnan, Guangdong, Guangxi, Fujian, Hainan and Taiwan, with a total output of 12.075238 million tons, ranking second in the world. Bananas are often infected by diseases during the production process, such as common scab, black spot, leaf spot, bunchy top, rot, and wilt, etc., which seriously affect the yield and quality of bananas. [0003] Such as banana bacterial soft rot ( Dickeya zeae ) was first discovered in Guangdong in 2009. More than 80% of the surveyed fields were infected with the disease, and the incidence rate ranged from 20-40%. Only from...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 周佳暖胡明李洁玲陈霞张炼辉徐领会
Owner SOUTH CHINA AGRI UNIV
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