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Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases

A technology of Bacillus velesi and Bacillus, applied in the application, chemicals and bacteria for biological control, etc., can solve the problems of imperfect bio-control bacteria resource pool and environmental pollution, and reduce the occurrence of banana diseases. , good control effect

Active Publication Date: 2018-12-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extensive use of chemical pesticides not only causes severe environmental pollution, but also makes pathogenic bacteria resistant to stress; the existing resource bank of bio-control bacteria is not yet perfect

Method used

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  • Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases
  • Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases
  • Bacillus velezensis and its broad spectrum bacteriostasis and use in prevention and treatment on banana diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and Screening of Bacillus 3-10

[0037] 1. Strain isolation

[0038] Sample collection: In the vegetable field in Huadu District, Guangzhou City, Guangdong Province, soil samples below 5 cm from the soil surface were excavated and stored in bags, and brought back to the laboratory for strain isolation.

[0039] Isolation of bacterial strains: Soil samples were isolated by dilution coating plate method. Specifically, after the collected soil samples were thoroughly mixed, 4.0 g was weighed, soaked in 20 mL of sterile water and shaken, and incubated at 28°C for 20 min on a shaker at 180 rpm for gradient dilution. Take the dilution factor as 10 5 -10 9 100 µL of the diluted solution was spread on the LB plate, air-dried at room temperature, sealed, and incubated upside down in a 28°C incubator for 24 h. Pick a single colony grown on the LB plate, label it, place it in a 2 mL centrifuge tube containing 500 µL of liquid LB, and culture it overnight at...

Embodiment 2

[0041] Example 2 Identification of Bacillus 3-10

[0042] 1. Morphological identification

[0043] Strain 3-10 is Gram-positive; it can reduce nitrate, liquefy gelatin, produce acid, peptone, produce catalase, amylase, and can utilize fructose, trehalose, glucose and sucrose.

[0044] 2. Molecular identification

[0045] In order to clarify the taxonomic status of strains 3-10 obtained in Example 1, we identified strains 3-10 using the method of multilocus sequence analysis (Multilocussequences analysis, MLSA) combined with phylogenetic analysis (Phylogenetic analysis).

[0046] 16S rDNA sequence analysis is a molecular biological identification method for bacterial identification. The present invention analyzes the 16S rDNA sequence and combines the conservative housekeeping gene gyrA and gyrB sequence, using MEGA 5.0 software to construct a phylogenetic tree using the Neighbor-joining method, and comprehensively analyzing the evolutionary relationship of 3-10, the resea...

Embodiment 3

[0049] Embodiment 3 Determination of the antibacterial spectrum of biocontrol bacteria 3-10

[0050] 1. In order to better study the biocontrol potential of bacterial strain 3-10 of the present invention, we have studied the bacteriostatic spectrum of this bacterium. The specific operation is as follows:

[0051] (1) Explore whether 3-10 has the ability to inhibit the growth of other pathogenic bacteria. Using the same method as in Example 1 strain screening, replace the pathogenic bacteria MS2 with the same amount of rice base rot pathogen EC1, banana bacterial soft rot MS3, and R. solanacearum EP1 (the first layer of 15 mL LB replaced with 15 mL of TTC), Brassicaceae black rot fungus Xcc, rice bacterial blight Xoo, peach rot fungus Pantoea ananatis PP1, yellow skin rot pathogen Pantoea anthophila CL1, the rest of the steps remain unchanged.

[0052] (2) Explore whether 3-10 has the ability to inhibit the growth of pathogenic fungi. The fungi tested included smut, down...

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Abstract

The invention discloses a broad-spectrum antibacterial effect of Bacillus velezensis 3-10 and its application in prevention and treatment on banana diseases. The strain is preserved in the Guangdong provincial microbial culture collection center on April 30, 2018 and has a preservation number of GDMCC NO. 60366. The strain 3-10 has broad-spectrum antibacterial effects on banana bacterial soft rotpathogen, Ralstonia solanacearum, xanthomonas campestris, peach rot pathogen, Clausena lansium rot pathogen, colletotrichum capsici, colletotrichum gloeosporioides, banana fusarium wilt pathogen, rhizoctonia solani, Xanthomonas oryzae pv. oryzae, peronophythora litchi, magaporthe grisea, ustilago maydis and Xanthomonas campestris pv., can significantly alleviate the occurrence of banana bacterialsoft rot and banana wilt, and has better effects in a pot experiment. The strain can be used to develop a broad spectrum of biological pesticide for preventing and treating important crop bacterial and fungal diseases and especially for preventing and treating banana diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. More specifically, a strain of Bacillus Velez ( Bacillus velezensis ) 3-10 and its broad-spectrum antibacterial effect and its application in the control of banana diseases. Background technique [0002] Banana is one of the four major fruits in the world. my country's banana production areas are mainly concentrated in Yunnan, Guangdong, Guangxi, Fujian, Hainan and Taiwan, with a total output of 12.075238 million tons, ranking second in the world. Bananas are often infected by diseases during the production process, such as common scab, black spot, leaf spot, bunchy top, rot, and wilt, etc., which seriously affect the yield and quality of bananas. [0003] Such as banana bacterial soft rot ( Dickeya zeae ) was first discovered in Guangdong in 2009. More than 80% of the surveyed fields were infected with the disease, and the incidence rate ranged from 20-40%. Only from...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 周佳暖胡明李洁玲陈霞张炼辉徐领会
Owner SOUTH CHINA AGRI UNIV
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