Marine invertebrate cell culture medium

Abstract

The invention discloses a marine invertebrate cell culture medium, which comprises a basic culture medium and a natural culture medium. The natural culture medium is sea cucumber coelomic fluid. The sea cucumber coelomic fluid comes from marine invertebrates, can construct a growth environment more similar to the body for marine invertebrate cell lines, so that marine invertebrate cell lines can make full use of the abundant nutrients contained in the sea cucumber coelomic fluid to achieve the purposes of rapid growth and stable passage. The invention develops the biomass resources of the aquatic commercial animal sea cucumber's processing by-product coelomic fluid, the sea cucumber coelomic fluid capable of replacing serum for application is prepared, is applicable to long-term culture ofmarine invertebrate cells, not only provides support for the culture of marine invertebrate cells, but also realizes waste utilization. The preparation cost of sea cucumber coelomic fluid is much lower than that of bovine serum, thus also greatly lowering the preparation cost of the marine invertebrate cell culture medium.

Description

technical field [0001] The invention belongs to the technical field of marine invertebrate cell culture, and in particular relates to a marine invertebrate cell culture medium. Background technique [0002] At present, the composition of animal cell culture medium mainly includes basal medium, natural medium (usually serum) and other regulatory components such as cytokines, among which serum is an essential source of nutrition and cell regulatory factors in cell culture. At present, the medium used to cultivate cells of land mammals and insects is relatively mature, but there are species differences between marine invertebrate cells and land animal cells, and the seawater environment in which marine invertebrates live will also cause cells to survive. The environmental requirements are quite different, so the culture conditions of marine invertebrate cells are quite different from those of mature terrestrial mammals, insects and other cells, and there is no stable and effect...

AI Technical Summary

Problems solved by technology

[0004] Although the medium uses the coelom fluid of C. monocircleum as a nutrient and cell regulation factor, which makes this medium more suitable for cultivating the trochophore larvae cell line of C. monocircleum than the traditional animal cell culture medium, but the medium still exists The following defects: (1) still need to use fetal bovine serum in the culture medium, and the usage amount of fetal bovine serum is also much larger than the cavity fluid of C. monocircleum, showing that although this medium can not utilize fetal bovine serum well Still have greater dependence on fetal bovine serum, and have not really obtained a stable and effective medium suitable for marine invertebrates; at the same time, fetal bovine serum is expensive, but it has not been fully utilized, resulting in greater waste; (2 ) This medium can only be used to cultivate the trochophore larvae cell line of C. monocircleum, and it is not suitable for other marine invertebrates, and the applicable range is narrow; Membrane filtration treatment without removing the immune molecules contained in it, and the immune molecules are likely to cause damage to the cultured objects if they are directly added to the culture medium

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