Notoginseng class sweet protein gene PnTLP5 and application thereof
A kind of sweet protein and gene technology, applied in the notoginseng sweet protein gene PnTLP5 and its application field, to achieve the effect of saving cost, reducing environmental pollution, and simple operation
Active Publication Date: 2019-01-18
KUNMING UNIV OF SCI & TECH
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The invention discloses a notoginseng disease course related protein 5 family sweet protein gene PnTLP5, which has a nucleotide sequence as described in SEQ IDNO: 1 and encodes a panax notoginseng type sweet protein as shown in SEQ IDNO: 2 amino acid sequence. The invention proves that the PnTLP5 gene has the function of improving plant anti-fungus through molecular biology and genetic engineeringrelated technology research, As that antifungal PnTLP5 gene of the invention is construct on a plant expression vector and transferred into tobacco for overexpression, Results The transgenic tobaccoplants had strong antifungal activity in vitro, and the transgenic tobacco overexpressing PnTLP5 had significant inhibitory effect on the growth of Sclerotinia sclerotiorum, Fusarium solani, Fusariumverticillatum, Alternaria ginseng and Botrytis citrina.
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Nicotiana tabacumFusarium solani +15
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