Preparation method of composite protein powder with high amino acid grade

A compound protein powder, high amino acid technology, applied in protein food processing, animal protein processing, vegetable protein processing and other directions, to achieve the effect of full and efficient utilization

Inactive Publication Date: 2019-04-26
刘良忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the above-mentioned protein powders currently on the market are not suitable for the elderly and patients with poor pro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] The rice protein and the egg white protein were prepared into a water suspension with a protein concentration of 8% at a mass ratio of 6:4, and the protein was denatured by heat treatment at 100°C for 10 minutes. The homogenization pressure was 30Mpa, and the temperature was 30°C. 3 times, colloid mill micronization treatment 3 times to make the protein particle size below 10μm; adjust the pH of the mixed protein solution to 2-3, temperature 35℃, papain and pepsin in a ratio of 1:1, the amount of enzyme added is that of the substrate protein 2%, ultrasonic frequency of 30kHz, power density of 100W / L, stirring speed of 30rpm, ultrasonic time of 60min, to obtain a protein hydrolysate with a degree of hydrolysis of 9%;

[0032] The protein hydrolysate is sterilized by ultra-high temperature at 121℃ for 9 seconds, cooled to 37℃, and the protease hydrolysate is added 10 per kilogram of fermentation broth. 8 The number of viable cfu bacteria, using Bacillus subt...

Example Embodiment

[0035] Example 2

[0036] Mix wheat protein, soy protein and duck egg white protein at a mass ratio of 7:2:1 to form a water suspension with a protein concentration of 6%. Heat at 95°C for 15 minutes to denature the protein and make the colloid mill finer. 4 Once again, make the protein particle size to 15μm.

[0037] Adjust the pH of the mixed protein solution to 9.5, the temperature of 45℃, the mass ratio of alkaline protease and trypsin at 1:1, the amount of enzyme added is 3% of the substrate protein, the ultrasonic frequency is 50kHz, the power density is 250W / L, the ultrasonic time is 60min, Obtain a protein hydrolysate with a degree of hydrolysis of 25%;

[0038] The protein hydrolyzate is sterilized at 95°C for 10 minutes and cooled to 38°C. The protease hydrolyzate is added to 10 per kilogram of fermentation broth. 7 The ratio of the number of cfu viable bacteria is combined fermentation of yeast-lactic acid bacteria, the ratio of yeast-lactic acid bacteria is 1:1, the yeas...

Example Embodiment

[0042] Example 3

[0043] Corn gluten, pea protein and oyster protein are mixed evenly according to the mass ratio of 5:4:1, prepared into a water suspension with a protein concentration of 5%, heated at 92°C for 16 minutes to denature the protein, and the high-pressure homogenizer is finely processed. The mass pressure is 40Mpa, the temperature is 50℃, and the number of times is 3 times, so that the size of the protein particles is about 10μm.

[0044] Adjust the mixed protein solution pH 7.5, temperature 37 ℃, neutral protease, papain and ficin in a mass ratio of 2:1:1, the amount of enzyme added is 4% of the substrate protein, ultrasonic frequency 40kHz, power density 150W / L , Ultrasonic time is 70min, and the protein hydrolysate with hydrolysis degree of 20% is obtained;

[0045] The protein hydrolysate is sterilized at 123°C for 15 seconds, cooled to 41°C, and the protease hydrolysate is added 5×10 per kilogram of fermentation broth. 6 The ratio of the number of cfu viable bact...

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Abstract

The invention provides a preparation method of composite protein powder with a high amino acid grade. The preparation method specifically comprises the following steps: performing micronization treatment on raw material protein by a colloid mill and/or a high-pressure homogenizer, heating the raw material protein to denature the protein, performing countercurrent ultrasonic assisted enzymatic hydrolysis through single protease or multi-enzyme combination, performing individual or joint fermentation of yeast, lactic acid bacteria and bacillus subtilis to remove stinking and peculiar smell, performing ultrafiltration and ion exchange resin desalting, evaluating and supplementing essential amino acids, performing enzymatic polymerization and reconstitution reaction, and performing drying or performing drying and mixing to obtain a high-quality protein product with a reasonable proportion of the essential amino acids and an amino acid grade being 95 or above. The obtained composite proteinpowder has the high essential amino acid grade, belongs to high-quality protein, is easy to digest and absorb, has low sodium content, is suitable for people who need the high-quality protein, elderly people with a low protein digestion function, and persons under rehabilitation; the protein powder with low contents of sodium, potassium and phosphorus is suitable for people suffering from chronickidney disease (CKD) and the like; the product also has the effects of resisting oxidation, lowering blood cholesterol, strengthening the body and enhancing immunity.

Description

technical field [0001] The invention relates to the technical field of food raw material processing, in particular to a method for preparing composite protein powder with high amino acid score. Background technique [0002] Protein is an indispensable substance in the human body, the material basis of life, the basic organic matter that constitutes cells, and the main bearer of life activities. Protein is one of the seven essential nutrients for the human body. Protein exists in various parts and cells of the body, and the content in the human body is about 17%-21%. Different proteins have different functions and properties. Protein functions include: forming and repairing human tissues, providing essential amino acids, regulating body fluids and maintaining acid-base balance, synthesizing physiologically active substances, enhancing immunity, and providing energy. There are many sources of protein, including grains, beans, eggs, meat, milk, aquatic products, etc. The Ch...

Claims

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Application Information

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IPC IPC(8): A23J3/34A23J3/04A23J3/08A23J3/14A23J3/16A23J3/18A23J3/20
CPCA23J3/04A23J3/08A23J3/14A23J3/16A23J3/18A23J3/20A23J3/341A23J3/343A23J3/346A23J3/347A23V2400/133A23V2400/121A23V2400/151A23V2400/137A23V2400/147A23V2400/143A23V2400/313A23V2400/231A23V2400/321A23V2400/413A23V2400/427A23V2400/125A23V2400/123A23V2400/513A23V2400/529A23V2400/517A23V2400/519A23V2400/175A23V2400/113A23V2400/533A23V2400/249A23V2400/169
Inventor 刘良忠王梦颖陶鑫
Owner 刘良忠
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