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Preparation method of composite coating with excellent biocompatibility and antibacterial property on surface of medical implant

A biocompatible and composite coating technology, applied in coatings, prostheses, medical science, etc., can solve problems such as high cost, lack of antibacterial properties of coatings, loose implants, etc., to achieve optimized surface morphology, Suitable for cell growth and enhanced antibacterial effect

Active Publication Date: 2019-07-23
BEIJING NATON TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of clinical studies have found that bacterial infection on the surface of implant materials is one of the important reasons leading to implant loosening and implant failure.
Cao Yazhou et al (Cao Yazhou, Cheng Wei, Hu Qingang. Effects of RGD / bionic calcium phosphate composite coating on titanium surface on adhesion, proliferation and differentiation of MC3T3-E1 cells[J]. Stomatological Research, 2017, 33(7): 693-697.) constructed RGD / bionic calcium phosphate composite coating on titanium surface by two-step immersion method. Studies have shown that the composite coating has a good ability to promote the proliferation and differentiation of osteoblasts, but the coating lacks antibacterial properties
Guo Yu et al. (Guo Yu, Liu Yong, Tan Yanni, et al. Mechanical properties and bioactivity of spark plasma sintered titanium-based tricalcium phosphate ceramic composites[J]. Powder Metallurgy Materials Science and Engineering, 2017(04):576- 584.) A titanium-based tricalcium phosphate composite material with high compressive strength and excellent biological activity can be obtained by using the spark plasma sintering method. This technology has a fast heating rate and high production efficiency, but the composite coating has no antibacterial properties and cannot inhibit bacteria Adhesion
There are also studies (Hu Yan, Peng Bingqing, Zhao Shuyun, etc. Preparation and antibacterial properties of silver-loaded hydroxyapatite [J]. Journal of Beijing Normal University (Natural Science Edition), 2007, 43(2): 163-165.) Combining multiple technologies to prepare silver-loaded hydroxyapatite composite coatings on titanium-based surfaces, the operation process is complicated and cumbersome, with high cost and low efficiency. Although silver has a significant antibacterial effect, the cytotoxicity and biocompatibility of silver and The bactericidal mechanism of nanosilver is still controversial

Method used

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  • Preparation method of composite coating with excellent biocompatibility and antibacterial property on surface of medical implant
  • Preparation method of composite coating with excellent biocompatibility and antibacterial property on surface of medical implant
  • Preparation method of composite coating with excellent biocompatibility and antibacterial property on surface of medical implant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Composite films of octacalcium phosphate and ε-polylysine were prepared by electrochemical co-deposition. The pure titanium foil was cut into a size of 1cm×1cm, ultrasonically cleaned in acetone, ethanol and deionized water for 15min, and then air-dried. Preparation solution: HF and HNO 3 The volume ratio is 1:10, and the pretreated titanium foil is immersed in the mixed acid solution for 2 minutes, rinsed with deionized water, and dried in the air. After removal, the deposition solution contained 10 mg / mL ε-polylysine, 0.014M Ca(NO 3 ) 2 and 0.04M NH 4 h 2 PO 4 , the treated titanium foil was used as the cathode, the graphite rod was used as the anode, and the constant current mode (0.5mA / cm 2 ) was electrodeposited on the surface of titanium foil to obtain a composite film of octacalcium phosphate and ε-polylysine, and the deposition time was 10 min.

[0030] figure 1 The scanning electron microscope images of the octacalcium phosphate and ε-polylysine composi...

Embodiment 2

[0032] Prepare the octacalcium phosphate and ε-polylysine composite film layer in Example 1 on the surface of pure titanium foil, and use the octacalcium phosphate film layer without ε-polylysine prepared under the same conditions as a comparison sample with WST -1 detection kit for cell proliferation test, using MC3T3-E1 cells, cell density 2×10 4 / cm 2 , and incubated for 1 day, 4 days, and 7 days. The result is as figure 2 As shown, the cells grow and adhere well on the surface of the octacalcium phosphate and ε-polylysine composite film, and have a certain ability to promote cell proliferation. When the cells were cultured for 7 days, the octacalcium phosphate and ε-polylysine composite film The cell proliferation rate is even higher than that of the octacalcium phosphate film layer, indicating that the composite film layer of octacalcium phosphate and ε-polylysine has good biocompatibility and biological activity.

Embodiment 3

[0034] Prepare the octacalcium phosphate and ε-polylysine composite film layer in Example 1 on the surface of pure titanium foil, and use the octacalcium phosphate film layer without ε-polylysine prepared under the same conditions as a comparison sample Pyridine orange staining was used for antibacterial experiments, using Escherichia coli and Staphylococcus aureus, cultured for 24 hours. The result is as image 3 and 4 As shown, due to the presence of ε-polylysine, the composite film layer of octacalcium phosphate and ε-polylysine significantly enhanced the inhibitory effect of the material surface on Escherichia coli and Staphylococcus aureus, making the implant have a strong Antibacterial properties.

[0035] The invention uses titanium, titanium alloy, tantalum alloy, stainless steel and other medical implants as the substrate, and constructs a composite film layer of octacalcium phosphate and ε-polylysine on the surface. Compared with the octacalcium phosphate film lay...

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Abstract

The invention discloses a preparation method of a composite coating with excellent biocompatibility and antibacterial property on the surface of a medical implant, and relates to the medical implant.The method comprises the following steps of pretreating a to-be-treated medical implant; putting the pretreated medical implant into a mixed acid solution to be etched; and preparing an calcium-phosphorus deposition solution containing epsilon-polylysine, and constructing octacalcium phosphate and epsilon-polylysine composite coating on the surface of the medical implant so as to obtain the composite coating with the excellent biocompatibility and antibacterial property on the surface of the medical implant. According to the method, in the construction process of introducing the epsilon-polylysine into an octacalcium phosphate film layer, the surface morphology of the octacalcium phosphate film layer can be optimized, so that growth and adhesion of cells can be facilitated, and the antibacterial property of the implant is remarkably improved on the basis that the excellent biological activity and biocompatibility of the octacalcium phosphate film layer are kept; and for different medical implants, the octacalcium phosphate and epsilon-polylysine composite coating can be directly constructed on the surfaces of different medical implants, so that the biological performance and the antibacterial property of the implant surface are effectively enhanced.

Description

technical field [0001] The invention relates to a medical implant, in particular to a method for preparing a composite coating with excellent biocompatibility and antibacterial properties on the surface of a medical implant. Background technique [0002] With the rapid development of biotechnology, medical implants have been widely used in artificial joints, bone plates and nails, vascular stents, human soft and hard tissue restorations, and artificial organs. Medical implants not only need to have good mechanical properties and physical and chemical properties, but also must have excellent biological activity. As an important component of human hard tissue, octacalcium phosphate has good biological activity and biocompatibility. Moreover, octacalcium phosphate is a precursor to form apatite, which can be converted into apatite spontaneously under normal physiological conditions, and combined with bone tissue, thereby significantly improving the biological activity of medic...

Claims

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Application Information

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IPC IPC(8): C23F1/16C25D9/04A61L27/34A61L27/32A61L27/06A61L27/04A61L27/54A61L31/10A61L31/02A61L31/16A61L31/08
CPCA61L27/04A61L27/06A61L27/32A61L27/34A61L27/54A61L31/022A61L31/088A61L31/10A61L31/16A61L2300/214A61L2300/404A61L2300/606A61L2420/02A61L2420/06A61L2430/02A61L2430/12C23F1/16C25D9/04C08L77/04
Inventor 林昌健胡洁洁张艳梅胡仁杨云樊丽丽段红平董骧
Owner BEIJING NATON TECH GRP CO LTD
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