A kind of fluorescent probe and its preparation method and application
A technology of fluorescent probes and compounds, applied in the field of fluorescent probe detection, can solve the problems of large ionic radius of iodide, low hydrogen bonding ability, low charge density, etc., and achieve the effects of strong selectivity, easy operation and high sensitivity
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Embodiment 1
[0036] A fluorescent probe, the molecular formula of the fluorescent probe is: C 90 h 81 N 3 o 9 ; The structural formula of the fluorescent probe is:
[0037]
[0038] This embodiment also provides a method for preparing the above-mentioned fluorescent probe, which method includes the following steps:
[0039]Step 1. Under the protection of nitrogen, add 500mg of compound A, 210mg of NaH and 25mL of dry tetrahydrofuran into a 50mL round bottom flask, heat and reflux at 100°C for 1 hour, add 780mg of N,N-diethyl -2-Chloroacetamide, continue to heat and reflux for 20 hours, distill off THF, add 20 mL of water to the refluxed product, adjust the pH to 7.0 with 1 mol / L hydrochloric acid solution, and then extract with 20 mL of ethyl acetate a 3 times, combined the organic phase a, washed the organic phase a 3 times with water, dried with anhydrous magnesium sulfate, and evaporated to dryness at a temperature of 100°C to obtain a yellow viscous substance, which was reconsti...
Embodiment 2
[0049] This embodiment provides the application of the fluorescent probe prepared in Example 1. The fluorescent probe is used to identify iodide ions, and the method for identifying iodide ions is:
[0050] Dissolve the fluorescent probe prepared in Example 1 in the blank stock solution, configure the fluorescent reagent with a concentration of 50 μmol / L of the fluorescent probe, pipette 2.4 mL of the blank stock solution into a quartz cuvette with a pipette gun, and then Add 300 μL fluorescent reagent and 300 μL aqueous solution with a concentration of 10 mmol / L to obtain a mixture solution with a fluorescent probe concentration of 5 μmol / L, perform fluorescence excitation at a wavelength of 346 nm, and identify iodide ions in the aqueous solution to be tested ;
[0051] The blank stock solution consists of tetrahydrofuran and 2 × 10 -3 The Tris-HCl buffer solution (trishydroxymethylaminomethane and hydrochloric acid mixture that the molar concentration ratio is 1:1) that th...
Embodiment 3
[0059] This embodiment is the anti-interference test when the fluorescent probe prepared in Example 1 is used to identify iodide ions:
[0060] Dissolve the fluorescent probe prepared in Example 1 in the blank stock solution, configure the fluorescent reagent with a concentration of 50 μmol / L of the fluorescent probe, pipette 2.1 mL of the blank stock solution into a quartz cuvette with a pipette gun, and then Add 300 μL of fluorescent reagent and 300 μL of iodide ion aqueous solution with a concentration of 10 mmol / L to obtain test solution A, and then add 300 μL of F with a concentration of 10 mmol / L to test solution A respectively. - Aqueous solution, Cl - Aqueous solution, Br - Aqueous solution, Aco - Aqueous solution, SO 3 2- Aqueous solution, SO 4 2- Aqueous solution, HSO 3 - Aqueous solution, NO 2 - aqueous solution and CO 3 2- Aqueous solution, obtain respectively test No. liquid B, test No. liquid C, test No. liquid, test No. E liquid, test No. F liquid, t...
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