A Peptide Fluorescent Probe Specifically Binding to Copper Ion and Cysteine

A fluorescent probe, species-specific technology, applied in the field of analysis and detection, can solve the problems of low probe sensitivity, poor biocompatibility, complicated preparation, etc., and achieve the effect of good biocompatibility, low toxicity, and simple preparation
CN110186891BActive Publication Date: 2020-05-15LANZHOU UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU UNIVERSITY
Publication Date
2020-05-15

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Abstract

The invention discloses a polypeptide fluorescence probe specifically binding cupric ions with cysteine. The amino acid sequence of the polypeptide is as shown in SEQ ID NO: 1; an N end of the polypeptide is modified with a fluorescent luminescent substance to form the polypeptide fluorescence probe P, the probe is capable of targeting and binding the Cu2+, has good selectivity, low toxicity and low detection limit, is simple to prepare and convenient to detect, and provides an efficient detection method for detecting cupric ions in biological and environmental samples; and the probe P is combined with the Cu2+ to form a P-Cu probe, and the probe can specifically identify Cys, and provide an efficient detection method for the rapid detection of the Cys in living bodies and environmental samples.
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Description

technical field

[0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a polypeptide fluorescent probe specifically binding to copper ions and cysteine. Background technique

[0002] Cu 2+ Cys (cysteine) is an indispensable small molecule in the human body and participates in the regulation of various physiological activities in the body. Cu 2+ It plays an important role in gene expression, immune regulation, and hematopoiesis, and an appropriate amount of Cu 2+ It will promote the physiological process of the body, but when Cu in the body 2+ When excessive, it will accumulate in the liver and cause liver cirrhosis, liver ascites, and diseases such as Alzheimer's disease and Down's syndrome are also related to the imbalance of copper metabolism in the body. Cys plays a vital role in biological functions such as protein synthesis and translation modification in organisms. When the Cys in the body is insufficient, it wi...

Claims

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