A fluorescent polymer microsphere loaded with quantum dots and its preparation method

Abstract

The invention discloses a fluorescent polymer microsphere with quantum dots inside and a preparation method thereof. First, acrylic acid and S‑1‑dodecyl‑S′‑(α,α′‑dimethyl‑α″‑acetic acid) trithiocarbonate are copolymerized to obtain a macromolecular RAFT agent PAA‑TTC; Glycidyl acrylate is a functional monomer, the macromolecular RAFT reagent PAA-TTC is a stable dispersant, and a cross-linking agent is added to synthesize cross-linked PGMA microspheres through the RAFT light dispersion polymerization method; then the obtained microspheres are placed in an organic solvent After swelling in medium, cadmium selenide quantum dots stabilized by mercaptopropionic acid ligands are added, and a catalyst is added under heating conditions to make the carboxyl groups on the mercaptopropionic acid ligands react with the epoxy groups inside the microspheres, thereby preparing the Polymer microspheres loaded with fluorescent quantum dots inside. By introducing epoxy groups inside the microspheres, they can react with water-soluble quantum dots stabilized by mercaptopropionic acid ligands, providing a basis for the preparation of quantum dot fluorescent polymer microspheres A new path has been developed, and the obtained fluorescent polymer microspheres have uniform particle size, good monodispersity, and very stable and long-lasting fluorescent properties.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a fluorescent polymer microsphere with quantum dots inside and a preparation method thereof. Background technique [0002] Polymer microspheres with sizes ranging from nanometers to micrometers, with fluorescent substances on the surface or inside, are widely used in biomedical detection, in vivo labeling, immunoassays, immobilized enzymes, etc. due to their stable morphology and high luminous efficiency. field has great application potential. At present, the preparation methods of fluorescent polymer microspheres mainly include physical and chemical methods. The physical method to prepare polymer fluorescent microspheres is the most direct and simplest method, often through the physical adsorption force on the surface of the microspheres, or hydrogen bond force, or embedding, encapsulation, etc., to combine fluorescent substances with polymer microspheres. The bigges...

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Problems solved by technology

The swelling method is simple to operate, but there is a problem of large differences in fluorescence brightness between individual fluorescent microspheres; the method of preparing quantum dot fluorescent microspheres by layer-by-layer self-assembly method is relatively simple, but it faces poor controllability, poor stability, and decreased fluorescence performance. and other problems; the fluorescent microspheres prepared by the copolymerization method have uniform brightness, and the quantum dots and microspheres are firmly combined, but the quantum dots are easily oxidized and aggregated during the polymerization process, which leads to a decrease in the fluorescence intensity of the quantum dots or even quenching

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