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Method for producing bio-organic fertilizer by degrading agricultural wastes with biological agent

A bio-organic fertilizer and agricultural waste technology, applied to fertilizers made from bio-waste, treatment of bio-organic parts, organic fertilizers, etc., can solve the problems of slow germination and growth of microbial spores, incomplete organic fertilizer nutrients, and degradation efficiency of raw materials Low-level problems, to achieve the effect of increasing production and income, increasing organic matter and total nutrient content of organic fertilizers, improving nutritional quality and functional value

Active Publication Date: 2020-09-04
DALIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problems existing in the prior art, the object of the present invention is to provide a method for producing bio-organic fertilizers by using biological bacterial agents to degrade agricultural wastes, aiming to solve the problems in the existing decomposing fermentation technology due to the nutrient substances in the decomposing and fermenting environment. Insufficient growth factors lead to slow germination and growth of microbial spores, low degradation efficiency of raw materials, insufficient decomposition, long fermentation time, and incomplete nutrients in organic fertilizers, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for producing bio-organic fertilizer by degrading agricultural waste using biological bacteria agents, the specific steps of preparing biological bacteria agents are as follows:

[0033] 1. Preparation of dry distiller's grains enzymatic hydrolysis solution:

[0034] Mix the dried distiller's grains with tap water at a mass ratio of 10%, adjust the pH to 9 with ammonia solution, bathe in water at 80°C for 60 minutes, and add alkaline protease to hydrolyze the dried distiller's grains at 400U / g for 24 hours at 50°C, then centrifuge That is, the dried distiller's grains enzymatic hydrolysis solution is obtained.

[0035] 2. Activation of strains

[0036] Bacillus subtilis was spread on LB (Luria-Bertani Agar) medium and cultured at 30°C for 20 hours to obtain Bacillus subtilis colonies; Cultivate for 96 hours to obtain Aspergillus niger and Trichoderma reesei spores; spread Saccharomyces cerevisiae on YPD (Yeast Extract PeptoneDextrose Agar) medium, and culture...

Embodiment 2

[0056] A method for producing bio-organic fertilizer by degrading agricultural waste with biological bacteria agent, the specific decomposing and fermenting steps are as follows:

[0057] 1. Mixed heap

[0058] Mix fresh cow dung, crushed straw and dried distiller's grains, adjust the carbon-to-nitrogen ratio to 30, wherein the amount of dried distiller's grains is 10% of the mass of fresh cow dung; add L-alanine fermentation waste liquid, stir well, pH7.8, Control water content 65%; Then add the biological bacteria agent prepared in embodiment 1 by adjusting 0.5% of the total mass of the heap after water content (the number of effective viable bacteria is 6.6 * 10 9 CFU / mL), mix well, and the total mass of the heap is close to 100kg.

[0059] 2. Decomposed and fermented

[0060]Use 100kg scale static decomposing fermentation. When the temperature rises to 55°C, do not turn the pile; when the temperature rises to 65°C, turn the pile every day to keep the temperature not high...

Embodiment 3

[0067] A method for producing bio-organic fertilizer by degrading agricultural waste with biological bacteria agent, the specific decomposing and fermenting steps are as follows:

[0068] 1. Mixed heap

[0069] Mix fresh cow dung, crushed wheat bran and dried distiller's grains, adjust the carbon-to-nitrogen ratio to 25, wherein the amount of dried distiller's grains is 5% of the mass of fresh cow dung; add L-alanine fermentation waste liquid, stir well, pH7.8 , control moisture content 55%; Then add the biological bacterial agent prepared in embodiment 1 (the number of effective viable bacteria is 6.6 * 109CFU / mL) by 0.1% of the total mass of the heap body after adjusting the moisture content, mix uniformly, the total mass of the heap body Close to 100kg.

[0070] 2. Decomposed and fermented

[0071] Use 100kg scale static decomposing fermentation. When the temperature rises to 55°C, do not turn the pile; when the temperature rises to 65°C, turn the pile every day to keep t...

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PUM

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for producing a bio-organic fertilizer by degrading agricultural wastes with a biological agent. The bio-organic fertilizer is prepared by mixing fresh cow dung, lignocellulose waste, dry vinasse and L-alanine fermentation waste liquid, adjusting the carbon-nitrogen ratio to 25-30, adjusting the moisturecontent to 55-65%, adding 0.1-0.5% of a biological agent, and performing thorough decomposition and fermentation for 6-18 days. The dry vinasse and enzymatic hydrolysate thereof are utilized to provide nutrient substances and growth promoting factors required in the functional microbial fermentation process, thereby enhancing the effective viable count in the biological agent and lowering the nutrient difference in the rotten fermentation environment; and the spore germination is accelerated by utilizing mycoprotein and L-alanine components in the L-alanine fermentation waste liquid. Various amino acids, vitamins and calcium phosphate rock substance components are effectively supplemented, the nutritional quality and functional titer of the bio-organic fertilizer are improved, and the yield and income increase and quality improvement of crops are facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing bio-organic fertilizer by degrading agricultural waste with a bio-bacteria agent. Background technique [0002] With the rapid development of modern agriculture in my country, a large amount of agricultural waste such as straw and livestock manure is produced, and the corresponding environmental pollution problems are gradually intensified, which is not conducive to the sustainable development of social economy. Among them, the annual production of agricultural straw lignocellulosic waste in my country exceeds 900 million tons, and the annual production of livestock and poultry manure reaches 3.8 billion tons, with great potential for resource utilization. Lignocellulosic waste is rich in organic matter, cellulose, crude protein, crude fat, and nutrients such as nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur. The nutrient content of...

Claims

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Application Information

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IPC IPC(8): C05G3/60C05F17/20
CPCC05C11/00C05F17/20C05G3/60C05F3/00C05F5/002C05F5/008C05F11/00C05F11/08C05F11/10Y02W30/40
Inventor 薛闯吴又多李苗苗
Owner DALIAN UNIV OF TECH