Lactobacillus acidipiscis strain and application thereof

A technology of lactobacillus and sour fish, which is applied in the direction of bacteria, application, biological water/sewage treatment, etc., can solve the problems of irregular production and market management, only focus on output and number of viable bacteria, and underestimate the actual application effect, etc., to achieve the realization of The effect of green development of agriculture, improvement of animal gastrointestinal function, and improvement of growth environment

Active Publication Date: 2020-11-06
浙江森井生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of agricultural microbial agents is relatively single, mainly consisting of several very traditional microorganisms such as Bacillus, yeast, and Clostridium.
Manufacturers also only pay attention to the output and the number of live bacteria and despise the actual application effect, and there are many irregularities in production and market management.

Method used

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  • Lactobacillus acidipiscis strain and application thereof
  • Lactobacillus acidipiscis strain and application thereof
  • Lactobacillus acidipiscis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Application of a Lactobacillus acidus strain:

[0024] a. Preparation medium: sucrose 1.5% (g / ml), yeast extract 1.5% (g / ml), sodium chloride 0.5% (g / ml), supplemented with water, and used after autoclaving;

[0025] b. Liquid submerged fermentation culture: insert a single colony of the strain into the above-mentioned culture medium, shake culture at 100r / min, the fermentation temperature is 35°C, the fermentation time is 48h, and the OD value of the fermentation broth is measured to be 7;

[0026] c. In 2019, a rice growth promotion test was conducted on the standard planting plot of China Rice Research Institute, Fuyang District, Hangzhou City, Zhejiang Province, and the fermentation broth of Lactobacillus acidi was applied to the soil in the form of base fertilizer, of which 20kg of fermentation broth was used per mu of land. , Start transplanting rice seedlings (seedling variety: Yongyou 12) one week after the application, harvest rice uniformly after the rice matu...

Embodiment 2

[0029] Application of a Lactobacillus acidus strain:

[0030] a. Preparation medium: sucrose 1.5% (g / ml), yeast extract 1.5% (g / ml), sodium chloride 0.5% (g / ml), supplemented with water, and used after autoclaving;

[0031] b. Liquid submerged fermentation culture: insert a single colony of the strain into the above-mentioned culture medium, shake culture at 100r / min, the fermentation temperature is 35°C, the fermentation time is 48h, and the OD value of the fermentation broth is measured to be 7;

[0032] c. In 2020, a wheat straw degradation test was conducted on the standard planting plot of China Rice Research Institute, Fuyang District, Hangzhou City, Zhejiang Province. The dry weight per mu is 300kg, the average length is about 10cm) and the quick-acting nitrogen fertilizer (urea 18kg) is ploughed together and applied to the soil. Bury a gauze mesh bag at 10-15cm underground, containing 100 wheat stalks in the fourth internode. One week after irrigation, before rice tra...

Embodiment 3

[0035] Application of a Lactobacillus acidus strain:

[0036] a. Preparation medium: sucrose 1.5% (g / ml), yeast extract 1.5% (g / ml), sodium chloride 0.5% (g / ml), supplemented with water, and used after autoclaving;

[0037] b. Liquid submerged fermentation culture: insert a single colony of the strain into the above-mentioned culture medium, shake culture at 100r / min, the fermentation temperature is 35°C, the fermentation time is 48h, and the OD value of the fermentation broth is measured to be 7;

[0038] c. In 2020, conduct the growth promotion test of Penaeus vannamei at the Zhejiang Provincial Freshwater Fisheries Research Institute Test Base in Pinghu City, Zhejiang Province, and mix the Lactobacillus acidi fermented liquid with the feed at each stage (purchased from Yangzhou Hongda Feed Co., Ltd., Shrimp feed) is used for feeding, wherein the amount of fermentation broth used is 1‰ (mL / g) of the feed, and the whole process is added since the feeding of the feed, and the ...

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Abstract

The invention relates to a lactobacillus acidipiscis strain and application thereof. The preservation number of the Lactobacillus acidipiscis strain is CGMCC No.20471, and the 16s rDNA base sequence of the strain is as shown in SEQ ID NO: 1. The invention also relates to a method for planting and breeding the strain. The method comprises the following specific steps: (1) a fermentation culture medium; (2) a fermentation method (including fermentation parameters and methods); (3) a fermentation liquid planting application method; and (4) a fermentation liquid culture application method. The strain has the beneficial effects that: the strain rapidly propagates in soil, straw, feed or aquaculture water, occupies the advantage of a micro-ecological region meanwhile by continuously inhibiting other microorganisms (including pathogenic bacteria), prevents harmful substances from being formed, improves the growth environment of plants, promotes straw decomposition and harmlessness of decomposition products, improves the gastrointestinal function of animals, and degrades nitrite in aquaculture water.

Description

technical field [0001] The present invention relates to a strain and application thereof, in particular to a Lactobacillus acidi strain and its application which can promote crop growth, degrade straw, promote animal growth and reduce nitrite accumulation in fish and shrimp culture water. Background technique [0002] At present, the excessive use of chemical fertilizers and pesticides is the main cause of non-point source pollution in the planting industry. At the same time, the bottleneck of comprehensive utilization of straw in my country still exists. On the one hand, it is necessary to prohibit the burning of straw to avoid air pollution. The domestic aquaculture industry is also entering a vicious circle: low-cost high-density breeding leads to poor breeding environment, poor quality of feed materials - poor disease resistance of animals - extensive use of antibiotics - antibiotics affect the digestive tract system of animals - gastrointestinal flora disorder and low i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/20A23K10/18A23K50/80C02F3/34C12R1/225C02F103/20C02F101/16
CPCA23K10/18A23K50/80C02F3/34C02F2101/166C02F2103/20C05F11/08C12N1/20C05F17/20C12N1/205C12R2001/225Y02W30/40
Inventor 刘侃诚
Owner 浙江森井生物技术股份有限公司
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