Hypoallergenic and anti-aging yak collagen composition and application thereof

A collagen and anti-aging technology, applied in the field of dermatology, can solve the problems of high endotoxin content, skin allergic reactions, induced skin diseases, etc., and achieve the effects of low cytotoxicity, promotion of cell proliferation, and low sensitization

Active Publication Date: 2021-10-22
胶原蛋白(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many commercially available collagen products currently have the problem of high endotoxin content. They are highly sensitizing, and can easily cause skin allergic reactions after contact with human skin, and even induce skin diseases.

Method used

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  • Hypoallergenic and anti-aging yak collagen composition and application thereof
  • Hypoallergenic and anti-aging yak collagen composition and application thereof
  • Hypoallergenic and anti-aging yak collagen composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 yak collagen

[0043] 1. Preparation method

[0044]Wash the yak tendons, remove foreign matter, and crush them into small pieces; treat the pulverized small pieces with 1% chlorhexidine and 5% hydrogen peroxide, take the precipitate, wash it with water until neutral; wash the obtained precipitate with 10 Degrease with % n-butanol, take the precipitate, wash with water until neutral; decalcify the precipitate obtained in the previous step with 0.5M hydrochloric acid, after decalcification is complete, take the precipitate, wash with water until neutral; the tissue precipitate obtained in the previous step Soak in 0.5M sodium hydroxide solution, stir at 10°C for 2 hours, take the precipitate, wash with water until neutral; extract collagen with 0.5M acetic acid solution containing pepsin to obtain a crude collagen extract; adjust pH to neutral The enzyme is inactivated; the dialysis bag is used for dialysis, and after the dialysis, it is ...

Embodiment 2

[0045] The quality evaluation of embodiment 2 yak collagen

[0046] 1. Determination of endotoxin content

[0047] Using ToxinSensor TM Chromogenic LAL endotoxin detection kit, and the standard curve of endotoxin content was obtained according to the method described in the kit manual; the endotoxin content was determined after each operation step in the yak collagen extraction process, and the determination method was sensitive Reliable, the measurement results are shown in Table 1 below.

[0048] Table 1 Test results of endotoxin content in each step of yak collagen preparation

[0049]

[0050] As can be seen from Table 1: the yak collagen extracted and prepared by the method of the present invention can significantly reduce the content of endotoxin in the yak collagen product, so that the content of endotoxin in the yak collagen product is lower than the detection limit, which is A yak collagen with good biosafety and hypoallergenicity.

[0051] 2. Characterization ...

Embodiment 3

[0066] Example 3 Yak Collagen Antioxidation Experiment

[0067] 1. DPPH free radical scavenging experiment process

[0068] Sample blank sample: Take 2ml of yak collagen prepared in Example 1 with different concentrations respectively, add 2ml of methanol to prepare Sample blank sample;

[0069] Control sample: Take 2ml of dissolved DPPH methanol solution and add 2ml of purified water to prepare the control sample;

[0070] The reference solution is methanol aqueous solution (methanol: water = 1:1);

[0071] Mix 2ml of DPPH methanol solution with 2ml of yak collagen solution of different concentrations to prepare sample test samples. After stirring vigorously for 1 minute, keep each of the above reaction mixtures in the dark at 28°C for 30 minutes; Measure the absorbance of the sample and each group of controls at 517nm, and calculate the DPPH free radical scavenging rate according to the following formula:

[0072] DPPH free radical scavenging rate (%)={1-(A sample test -...

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Abstract

The invention belongs to the field of skin medicine, and particularly relates to a hypoallergenic and anti-aging yak collagen compound. The invention provides the yak collagen with low endotoxin and low sensitization, and the yak collagen is low in cytotoxicity, can promote cell proliferation and cell adhesion, and has good properties such as oxidation resistance and aging resistance. Meanwhile, the yak collagen composition containing yak collagen, hyaluronic acid, dipotassium glycyrrhizinate, acetyl hexapeptide-8, ellagic acid, a Salix alba bark extract, nicotinamide, vitamin E and resveratrol is obtained through screening, and the yak collagen composition has low sensitization and more excellent antioxidant and anti-aging performance, and can be widely applied to the field of cosmetics or dermatology.

Description

technical field [0001] The invention belongs to the field of dermatology, and in particular relates to a hypoallergenic and anti-aging yak collagen composition and its application. Background technique [0002] Collagen is the most abundant protein in the human body, accounting for about 30% of the total protein in the human body. Collagen is the main component of the extracellular matrix and the main component of the skin. It is widely distributed in the skin, blood vessels, bones, tendons, corneas and other connective tissues of the human body. At present, 29 different types of collagen have been found, among which the dermis of human skin mainly contains type I collagen, and the fibrous scaffold formed by the assembly of type I collagen provides elasticity and strength for the skin. [0003] Collagen, a natural component of the skin, has been used extensively in cosmetics and skin repair dressings. The tyrosine residues in the collagen fight against the tyrosine in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/65A61K8/63A61K8/9789A61K8/49A61K8/34A61K8/64A61K8/67A61K8/73A61Q19/08A61Q19/00A61K38/39A61P17/18A61P37/08C07K14/78C12P21/06C07K1/30C07K1/14A61K31/704A61K36/76A61K31/366A61K31/05A61K38/08A61K31/047A61K31/355A61K31/728
CPCA61K8/65A61K8/63A61K8/9789A61K8/498A61K8/347A61K8/64A61K8/345A61K8/678A61K8/735A61Q19/08A61Q19/005A61K38/39A61K31/704A61K36/76A61K31/366A61K31/05A61K38/08A61K31/047A61K31/355A61K31/728A61P17/18A61P37/08C07K14/78C12P21/06A61K2300/00
Inventor 肖建喜付彩虹
Owner 胶原蛋白(武汉)生物科技有限公司
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