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Nanometer mesoporous carbon eye drops loaded with natamycin and silver as well as preparation method and application thereof

A nano-mesoporous and natamycin technology, applied in nano-carbon, nano-technology for materials and surface science, nano-technology, etc., can solve the high blinding rate of fungal keratitis and ocular surface residence time Short, strong irritation and other problems, to achieve the effect of improving solubility and bioavailability, prolonging antifungal time, and easy storage

Active Publication Date: 2021-10-22
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antifungal eye drops currently used clinically have problems such as poor water solubility, short ocular surface residence time, strong irritation, and high toxicity. The lack of treatment methods leads to a high blinding rate of fungal keratitis. Therefore, looking for fungal keratitis New dosage forms of inflammation treatment drugs are crucial
However, there is no report on the application of nano-mesoporous carbon materials in ophthalmic drugs

Method used

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  • Nanometer mesoporous carbon eye drops loaded with natamycin and silver as well as preparation method and application thereof
  • Nanometer mesoporous carbon eye drops loaded with natamycin and silver as well as preparation method and application thereof
  • Nanometer mesoporous carbon eye drops loaded with natamycin and silver as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment relates to the preparation method of nano-mesoporous carbon eye drops loaded with natamycin and silver, and the specific steps are as follows:

[0035] 1) Dissolve 5g of sodium alginate in 500mL of double-distilled water, stir at room temperature for 4 hours, configure a sodium alginate solution with a mass volume concentration of 10mg / mL, and drop it into 500mL of calcium chloride with a concentration of 0.1mol / L solution, stirred at room temperature for 8 hours, and filtered through gauze to obtain calcium alginate gel;

[0036] 2) Put the calcium alginate gel in a freeze dryer (-60°C) for 48 hours, then put it into a tube furnace, heat it to 950°C in a nitrogen atmosphere (the heating rate is 5°C / min), and keep it warm for 1 Hour;

[0037] 3) Place the carbonized sample in a hydrochloric acid solution with a molar concentration of 1 mol / L, soak it overnight at room temperature, then filter and wash it with double distilled water and ethanol, and plac...

Embodiment 2

[0051] This embodiment relates to the preparation method of nano-mesoporous carbon eye drops loaded with natamycin and silver, and the specific steps are as follows:

[0052] 1) Dissolve 2.5g of sodium alginate in 500mL of double-distilled water, stir at room temperature for 4 hours, configure a sodium alginate solution with a density of 5mg / mL, and drop it into 500mL of calcium chloride with a molar concentration of 0.1mol / L In the aqueous solution, stir at room temperature for 8 hours, and obtain calcium alginate gel after filtering through gauze;

[0053] 2) Put the calcium alginate gel in a freeze dryer (-60°C) for 48 hours, then put it into a tube furnace, heat it to 950°C in a nitrogen atmosphere (the heating rate is 5°C / min), and keep it warm for 1 Hour;

[0054] 3) Place the carbonized sample in 1mol / L aqueous hydrochloric acid solution, soak overnight at room temperature, filter and wash with double distilled water and ethanol, and place it in a blast dryer (60°C) fo...

Embodiment 3

[0059] This embodiment relates to the drug release ability and influencing factors analysis experiment of nano mesoporous carbon loaded with natamycin, specifically:

[0060] (1) In vitro drug release experiment

[0061] Weigh 2 mg of natamycin-loaded nano-mesoporous carbon, add 3 mL of PBS solution, put it into a dialysis bag, clamp both ends, and put it into a beaker with 50 mL of PBS solution at a temperature of 37 ° C and a rotation speed of 100 r / min, take out 2mL of dialysate every 2 hours, and immediately replenish the same amount of PBS solution, measure the absorbance of the dialysate at 304nm, calculate its concentration according to the regression equation, and calculate the cumulative drug release rate: cumulative drug release rate = Released drug amount / administered amount × 100%. The result is as figure 2 As shown in A.

[0062] From figure 2 It can be seen from A that the release of natamycin presents sustained release, with a release rate of about 56% in...

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Abstract

The invention relates to the technical field of medical materials, in particular to natamycin and silver loaded nano mesoporous carbon eye drops as well as a preparation method and application thereof. The preparation method comprises the following steps: loading silver on the nano mesoporous carbon, loading natamycin on the nano mesoporous carbon, and preparing the eye drops. Herein, the nano mesoporous carbon is used as a carrier to load natamycin and silver, the nano mesoporous carbon has the characteristics of high drug loading capacity and large specific surface area, the purpose of slowly releasing natamycin can be achieved, and the solubility and bioavailability of natamycin can be improved; therefore, the medication frequency of a fungal keratitis patient is reduced, the compliance of the patient is improved, and the treatment effect is improved; meanwhile, the nano mesoporous carbon can also adsorb inflammatory factors into an internal carbon structure, so that inflammatory response is effectively relieved; in addition, the silver elementary substance has a strong fungus killing effect, the antifungal time of the silver elementary substance can be prolonged by loading the silver elementary substance into the nano mesoporous carbon, meanwhile, the dosage of natamycin is reduced, and adverse reactions are relieved. The preparation method is simple, low in cost and easy to store.

Description

Technical field: [0001] The invention relates to the technical field of medical materials, and relates to a nano mesoporous carbon eye drop loaded with natamycin and silver and its preparation method and application. The nano mesoporous carbon is used as a carrier to load natamycin and silver. Improve the solubility and bioavailability of natamycin, reduce the dosage of natamycin, and reduce adverse reactions. [0002] technical background: [0003] Fungal keratitis is an infectious eye disease related to pathogenic fungi. Fusarium and Aspergillus are the main pathogens of fungal keratitis in my country, which can cause serious visual impairment. As a developing country with industry and agriculture as the main industries in my country, the incidence of fungal keratitis has increased significantly in recent years, ranking first in my country's infectious keratitis. Most of the patients with the disease are young and middle-aged laborers working in the front line, with high b...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K47/04A61K31/7048A61K33/38A61P27/02A61P31/10C01B32/15B82Y30/00B82Y40/00
CPCA61K9/08A61K9/0048A61K47/02A61K31/7048A61K33/38A61P27/02A61P31/10C01B32/15B82Y30/00B82Y40/00A61K2300/00Y02A50/30
Inventor 李翠赵桂秋顾凌雯李道浩姜楠徐强
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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