Fibroin/alginate fiber-based composite transparent hydrocolloid dressing with biological activity and preparation method thereof
A technology of seaweed fiber and silk fibroin, which is applied in pharmaceutical formulations, drug delivery, medical science and other directions, can solve the problems of restricting the application and development of alginic acid dressings, slow biodegradation, poor mechanical properties, etc., and achieves good biodegradability. , Easy to operate, stable sticking effect
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Embodiment 1
[0039] The preparation of embodiment 1 silk fiber solution
[0040]Select high-quality silkworm cocoons, cut them into pieces, and perform ultrasonic cleaning to remove impurities, and then put them into 2L of 10wt% sodium carbonate solution and boil them for 30 minutes to degumming to obtain silk fibroin fibers. Wash the degummed silk fibroin with deionized water After drying, put 3g of silk fibroin into the prepared sodium hydroxide / urea mixed solution (9g of sodium hydroxide and 12g of urea are added to ultrapure water and stirred to 100ml, then put into -12 ℃ refrigerator), after stirring the silk fibroin, put it back into the refrigerator to dissolve, and dissolve it for a total of three days, wherein the beaker is taken out and stirred for 20 minutes every half day, and then put back into the refrigerator.
[0041] After the dissolution, put the eluate into a pretreated dialysis bag (molecular weight cut-off 7000) and dialyze in 2L ultrapure water for three days to remov...
Embodiment 2
[0042] The preparation of embodiment 2 novel composite transparent hydrocolloid dressings
[0043] 1. Preparation: Adjust the concentration of the silk fiber solution extracted in Example 1 to 2% (W / V) and place it in a refrigerator at 4°C for standby; at the same time, configure the same alginic acid with a concentration of 2% (W / V) Sodium solution: Add 2g of sodium alginate powder (Sanko, CAS: [9005-38-3]) into 50ml of ultrapure water and stir on a magnetic stirrer at 50°C at a speed of 500rpm for 6-8h to dissolve, then dilute to 100ml , Store in a refrigerator at 4°C for later use.
[0044] 2. Preparation: The preparation process is as follows: figure 1 As shown, the prepared silk fiber solution and sodium alginate solution were evenly mixed into three solutions in different volume ratios (3:1, 1:1, 1:3) and the total volume of each solution was kept at 20ml. The composite dressings prepared according to the aforementioned ratios were named as F3A1, F1A1, and F1A3, and th...
Embodiment 3
[0046] Example 3 Exploration of the Optimal Proportion of Composite Dressing and Verification of its Biological Activity
[0047] Various characterizations and tests such as scanning electron microscope test, light transmittance test, tensile test, degradation test and air permeability test were carried out on the composite dressings prepared in three different proportions prepared in Example 2, and the most suitable properties were selected by comparison. As a ratio of skin dressing, when the content of silk seaweed is equivalent, it is found that the composite dressing has the best properties as a wound dressing. Therefore, based on this, further in vivo and in vitro biological applications such as cytotoxicity experiments, hemostasis experiments and small Rat full-thickness skin lesion model to verify its biological activity.
[0048] 1. Composite dressing scanning electron microscope observation
[0049] Firstly, the composite dressings with different proportions were fre...
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