High-sensitivity non-enzymatic glucose sensor and preparation method thereof

A glucose sensor and high-sensitivity technology, applied in the field of glucose sensors, can solve the problems of low working voltage biocompatibility and stability, poor biocompatibility of nano-copper oxide, low detection limit and working voltage, etc., and achieve good biological phase. Capacitive and corrosion resistance, large detection linear range, small detection limit effect

Pending Publication Date: 2022-04-12
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AI-Extracted Technical Summary

Problems solved by technology

"Platinum and zinc oxide modified carbon nitride electrodeas non-enzymatic highly selective and reusable electrochemical diabetic sensor in human blood" produced a platinum and zinc oxide modified carbon nitride electrode as a non-enzymatic glucose sensor, which has a wide detection linear range , lower detection limit, but the sensitivity is too low, and the higher operating voltage also affects the selectivity of detection
Nano-copper oxide has poor biocompatibility, and nickel foam also has certain biotoxicity
In addition, the preparation process of these non-enzyme sensors and the first three generations of enzyme sensors is very cumbersome and requires multi-step modification, which is extremely unfavorable for further res...
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The invention provides a high-sensitivity non-enzymatic glucose sensor and a preparation method thereof. The high-sensitivity non-enzymatic glucose sensor is formed by sintering a gold and bovine serum albumin film on the surface of fluorine-doped tin oxide conductive glass. The specific operation comprises the steps that a chloroauric acid solution is taken and added into a bovine serum albumin solution, the pH value is adjusted to be alkaline after stirring, ascorbic acid is added, centrifugation and washing are conducted after sufficient reaction, and obtained precipitates are dispersed in deionized water and placed in a low-temperature environment for aging for use. Taking the fully aged prepared solution, dropwise adding the fully aged prepared solution on fluorine-doped tin oxide conductive glass to form round liquid drops, drying, sintering at high temperature, and finally naturally cooling to room temperature to obtain the high-sensitivity non-enzymatic glucose sensor. The sensor has the advantages of being large in detection linear range, high in sensitivity, low in detection limit, good in stability and the like.

Application Domain

Material electrochemical variables

Technology Topic

AlbuminTin oxide +12


  • High-sensitivity non-enzymatic glucose sensor and preparation method thereof
  • High-sensitivity non-enzymatic glucose sensor and preparation method thereof
  • High-sensitivity non-enzymatic glucose sensor and preparation method thereof


  • Experimental program(3)

Example Embodiment

[0027] The preparation of embodiment 1 gold @ bovine serum albumin
[0028] A. Slowly drop 10 ml of chloroauric acid solution with a concentration of 10 mmol/L into the stirring 10 ml of bovine serum albumin solution with a concentration of 5 mg/mL, and stir for 5 min.
[0029] B. Slowly add sodium hydroxide solution with a concentration of 0.2 mol/L dropwise, adjust the pH value to 12, and stir for 1 min.
[0030] C. Quickly add 50 mg of ascorbic acid and stir for 5 min.
[0031] D. Centrifuge the fully reacted solution at 8000r/min for 10min, wash the precipitate with deionized water and centrifuge, repeat the washing and centrifugation twice, finally prepare 10ml of deionized water, disperse the centrifuged sediment and seal it, As for aging at about 4°C low temperature for 24h, for use, gold@bovine serum albumin solution was obtained.

Example Embodiment

[0032] Example 2 Preparation of high-sensitivity non-enzymatic glucose sensor
[0033] A. Using fluorine-doped tin oxide conductive glass as the base, the size is 25×8×1.1mm. Take 10 μL of the prepared gold@bovine serum albumin solution and drop it on the fluorine-doped tin oxide glass to form a circular droplet with a diameter of about 5 mm.
[0034] B. Dry the fluorine-doped tin oxide conductive glass with droplets at 50°C until the droplets are completely dried, forming a dark blue circular film.
[0035]C. The fluorine-doped tin oxide conductive glass dried in the previous step is calcined at a high temperature of 400° C. for 2 hours, and after cooling to room temperature, the film exhibits an obvious golden yellow color. So far, the preparation of a high-sensitivity non-enzymatic glucose sensor of the present invention is completed.

Example Embodiment

[0036] Embodiment 3 Detection experiment
[0037] The detection experiment was carried out on a CHI600E electrochemical workstation and a three-electrode system. The working electrode was the high-sensitivity non-enzymatic glucose sensor of the present invention, the counter electrode was a platinum wire electrode, and the reference electrode was a saturated calomel electrode. First, activate the sensor in sulfuric acid with a concentration of 0.1mol/L, that is, scan the cyclic voltammetry curve. The starting and ending voltages are 0V and 1.5V, respectively, and the cycle is repeated for more than 20 cycles, so that the adjacent two curves almost overlap. activation. Then, the performance of the sensor of the present invention was characterized by the single-potential step chronoamperometry. Under the action potential of 0.15V, glucose was continuously added to the test bottom solution, and the current time curves at different concentrations were measured, as shown in the following figure: figure 1 , figure 2 and image 3 shown. Finally, according to the above three time-current time curves, it can be found that the high-sensitivity non-enzymatic glucose sensor of the present invention has three different linear ranges, such as Figure 4 shown. The standard curves of glucose concentration and response current in the three linear ranges are as follows: Figure 5 0.1~1mmol/L shown, the sensitivity is 330.002μA/mmol/cm 2; Image 6 1~8mmol/L shown, the sensitivity is 103.786μA/mmol/cm 2; Figure 7 8~34mmol/L shown, the sensitivity is 53.738μA/mmol/cm 2. The goodness of fit R of the three-segment regression equation 2 were 0.998, 0.997, and 0.998, respectively, and the detection limit was 3.6 μmol/L.


Particle size50.0 ~ 200.0nm

Description & Claims & Application Information

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