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High-sensitivity non-enzymatic glucose sensor and preparation method thereof

A glucose sensor and high-sensitivity technology, applied in the field of glucose sensors, can solve the problems of low working voltage biocompatibility and stability, poor biocompatibility of nano-copper oxide, low detection limit and working voltage, etc., and achieve good biological phase. Capacitive and corrosion resistance, large detection linear range, small detection limit effect

Pending Publication Date: 2022-04-12
BEIFANG UNIV OF NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Platinum and zinc oxide modified carbon nitride electrodeas non-enzymatic highly selective and reusable electrochemical diabetic sensor in human blood" produced a platinum and zinc oxide modified carbon nitride electrode as a non-enzymatic glucose sensor, which has a wide detection linear range , lower detection limit, but the sensitivity is too low, and the higher operating voltage also affects the selectivity of detection
Nano-copper oxide has poor biocompatibility, and nickel foam also has certain biotoxicity
In addition, the preparation process of these non-enzyme sensors and the first three generations of enzyme sensors is very cumbersome and requires multi-step modification, which is extremely unfavorable for further research and development.
[0004] Existing non-enzymatic glucose sensors based on gold have low operating voltage and good biocompatibility and stability, but there are still some problems, such as "Ternary nanocomposites of Au / CuS / TiO2 for anultrasensitive photoelectrochemicalnon-enzymatic The sensor prepared in "glucose sensor" has high sensitivity, but the detection limit is too high, the detection linear correlation range is small, and the preparation is complicated
The sensor prepared by "Porous goldcluster film prepared from Au@BSA microspheres for electrochemicalnonenzymatic glucose sensor" has a lower detection limit and working voltage, but the sensitivity is too low, and the detection linear correlation range is also small

Method used

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  • High-sensitivity non-enzymatic glucose sensor and preparation method thereof
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  • High-sensitivity non-enzymatic glucose sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of Example 1 Gold@Bovine Serum Albumin

[0028] A. Slowly add 10ml of chloroauric acid solution with a concentration of 10mmol / L dropwise into 10ml of bovine serum albumin solution with a concentration of 5mg / mL in stirring, and stir for 5min.

[0029] B. Slowly add sodium hydroxide solution with a concentration of 0.2 mol / L dropwise, adjust the pH value to 12, and stir for 1 min.

[0030] C. Quickly add 50 mg of ascorbic acid and stir for 5 minutes.

[0031] D. Centrifuge the fully reacted solution at a speed of 8000r / min for 10min, wash the precipitate with deionized water and centrifuge, repeat the washing and centrifugation twice, and finally prepare 10ml of deionized water, disperse the centrifuged precipitate and seal it. As for aging at a low temperature of about 4°C for 24 hours, set aside for later use to obtain a gold@bovine serum albumin solution.

Embodiment 2

[0032] Embodiment 2 Preparation of high-sensitivity non-enzyme glucose sensor

[0033] A. Based on fluorine-doped tin oxide conductive glass, the specification is 25×8×1.1mm. Take 10 μL of the prepared gold@bovine serum albumin solution and drop it on the fluoride-doped tin oxide glass to form a circular drop with a diameter of about 5 mm.

[0034] B. Dry the fluorine-doped tin oxide conductive glass with droplets on it at 50°C until the droplets are completely dry to form a dark blue circular film.

[0035]C. Calcining the fluorine-doped tin oxide conductive glass dried in the previous step at a high temperature of 400°C for 2 hours, and after cooling to room temperature, the film takes on an obvious golden yellow color. So far, a high-sensitivity non-enzymatic glucose sensor of the present invention has been prepared.

Embodiment 3

[0036] Embodiment 3 detection experiment

[0037] The detection experiment was carried out on a CHI600E electrochemical workstation and a three-electrode system. The working electrode is the high-sensitivity non-enzymatic glucose sensor of the present invention, the counter electrode is a platinum wire electrode, and the reference electrode is a saturated calomel electrode. First, activate the sensor in sulfuric acid with a concentration of 0.1mol / L, that is, scan the cyclic voltammetry curve. The start and end voltages are 0V and 1.5V respectively, and the cycle is more than 20 cycles, so that the adjacent two curves are almost overlapped, so far. activation. Then, use the single potential step chronoamperometry to characterize the sensor performance of the present invention, under the action potential of 0.15V, add glucose continuously in the bottom liquid of test, measure the current time curve under different concentrations, as figure 1 , figure 2 and image 3 shown. ...

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Abstract

The invention provides a high-sensitivity non-enzymatic glucose sensor and a preparation method thereof. The high-sensitivity non-enzymatic glucose sensor is formed by sintering a gold and bovine serum albumin film on the surface of fluorine-doped tin oxide conductive glass. The specific operation comprises the steps that a chloroauric acid solution is taken and added into a bovine serum albumin solution, the pH value is adjusted to be alkaline after stirring, ascorbic acid is added, centrifugation and washing are conducted after sufficient reaction, and obtained precipitates are dispersed in deionized water and placed in a low-temperature environment for aging for use. Taking the fully aged prepared solution, dropwise adding the fully aged prepared solution on fluorine-doped tin oxide conductive glass to form round liquid drops, drying, sintering at high temperature, and finally naturally cooling to room temperature to obtain the high-sensitivity non-enzymatic glucose sensor. The sensor has the advantages of being large in detection linear range, high in sensitivity, low in detection limit, good in stability and the like.

Description

technical field [0001] The invention relates to the technical field of glucose sensors, in particular to a high-sensitivity non-enzymatic glucose sensor and a preparation method thereof. Background technique [0002] As a chronic disease, the incidence of diabetes in the population is increasing year by year because it does not receive enough attention. According to the 2019 news from the National Health and Medical Commission of China, my country has more than 97 million diabetic patients and about 150 million pre-diabetic patients. It is one of the countries with the fastest growing prevalence of diabetes in the world. Diabetes has become a major hidden danger to human health. Rapid and accurate determination of glucose content in human blood is extremely important for the prevention and treatment of diabetes. [0003] The development of glucose sensors has basically gone through four generations. The first three generations belong to enzymatic glucose sensors. These sen...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/36
Inventor 李维银赵瞳瞳丁玲玲
Owner BEIFANG UNIV OF NATITIES
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