High-sensitivity non-enzymatic glucose sensor and preparation method thereof

Abstract

The invention provides a high-sensitivity non-enzymatic glucose sensor and a preparation method thereof. The high-sensitivity non-enzymatic glucose sensor is formed by sintering a gold and bovine serum albumin film on the surface of fluorine-doped tin oxide conductive glass. The specific operation comprises the steps that a chloroauric acid solution is taken and added into a bovine serum albumin solution, the pH value is adjusted to be alkaline after stirring, ascorbic acid is added, centrifugation and washing are conducted after sufficient reaction, and obtained precipitates are dispersed in deionized water and placed in a low-temperature environment for aging for use. Taking the fully aged prepared solution, dropwise adding the fully aged prepared solution on fluorine-doped tin oxide conductive glass to form round liquid drops, drying, sintering at high temperature, and finally naturally cooling to room temperature to obtain the high-sensitivity non-enzymatic glucose sensor. The sensor has the advantages of being large in detection linear range, high in sensitivity, low in detection limit, good in stability and the like.

Description

technical field [0001] The invention relates to the technical field of glucose sensors, in particular to a high-sensitivity non-enzymatic glucose sensor and a preparation method thereof. Background technique [0002] As a chronic disease, the incidence of diabetes in the population is increasing year by year because it does not receive enough attention. According to the 2019 news from the National Health and Medical Commission of China, my country has more than 97 million diabetic patients and about 150 million pre-diabetic patients. It is one of the countries with the fastest growing prevalence of diabetes in the world. Diabetes has become a major hidden danger to human health. Rapid and accurate determination of glucose content in human blood is extremely important for the prevention and treatment of diabetes. [0003] The development of glucose sensors has basically gone through four generations. The first three generations belong to enzymatic glucose sensors. These sen...

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Problems solved by technology

"Platinum and zinc oxide modified carbon nitride electrodeas non-enzymatic highly selective and reusable electrochemical diabetic sensor in human blood" produced a platinum and zinc oxide modified carbon nitride electrode as a non-enzymatic glucose sensor, which has a wide detection linear range , lower detection limit, but the sensitivity is too low, and the higher operating voltage also affects the selectivity of detection

Nano-copper oxide has poor biocompatibility, and nickel foam also has certain biotoxicity

In addition, the preparation process of these non-enzyme sensors and the first three generations of enzyme sensors is very cumbersome and requires multi-step modification, which is extremely unfavorable for further research and development.

[0004] Existing non-enzymatic glucose sensors based on gold have low operating voltage and good biocompatibility and stability, but there are still some problems, such as "Ternary nanocomposites of Au / CuS / TiO2 for anultrasensitive photoelectrochemicalnon-enzymatic The sensor prepared in "glucose sensor" has high sensitivity, but the detection limit is too high, the detection linear correlation range is small, and the preparation is complicated

The sensor prepared by "Porous goldcluster film prepared from Au@BSA microspheres for electrochemicalnonenzymatic glucose sensor" has a lower detection limit and working voltage, but the sensitivity is too low, and the detection linear correlation range is also small

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