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Promoter for the production of hyaluronic acid containing ginsenoside compound k

a technology of ginsenoside and hyaluronic acid, which is applied in the direction of biocide, dermatological disorders, drug compositions, etc., can solve the problems of accelerating cartilage damage, hyaluronic acid metabolism mechanism has not yet been fully elucidated, and studies on the distribution of hyaluronic acid in cells and tissues, so as to promote the production of hyaluronic acid and increase the expression of the hyaluronic acid synth

Inactive Publication Date: 2006-07-20
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Benefits of technology

[0008] Under these circumstances, the present inventors researched to find an effective method for supplying hyaluronic acid into the human body. As a result thereof, we found that compound K (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol), a chief metabolite of ginsepeng saponin having immunity-increasing, tumor angiogenesis-suppressing and cancerous cell permeation-inhibiting effects, can increase the expression of gene cording hyaluronic acid synthase in human cells and thereby promote the production of hyaluronic acid in the human body. That is, the production of hyaluronic acid can be promoted by treatment with compound K, resulting in increasing the concentration of hyaluronic acid in the human body. This result suggests that compound K can be applied to various purposes utilizing the efficacy of hyaluronic acid such as skin-care uses for improvement of skin elasticity and prevention of skin drying or skin aging and pharmaceutical uses for treatment or prevention of osteoarthritis. Based on this finding, the present invention has been completed.
[0009] Therefore, an object of the present invention is to provide a new use of compound K to increase the expression of the hyaluronic acid synthase gene and thereby to promote the production of hyaluronic acid.

Problems solved by technology

Therefore as the concentration of hyaluronic acid in a joint decreases, the joint cannot absorb or disperse shocks, so accelerating cartilage damage.
However, the detailed mechanism for the metabolism of hyaluronic acid has not yet been fully elucidated.
However, studies on distribution of hyaluronic acid in cells and tissues and on various factors and enzymes related to hyaluronic acid, for example HAS or factors regulating the activity of hyaluronic acid, remain insufficient up to now.
However, obvious results are not yet known.

Method used

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  • Promoter for the production of hyaluronic acid containing ginsenoside compound k
  • Promoter for the production of hyaluronic acid containing ginsenoside compound k
  • Promoter for the production of hyaluronic acid containing ginsenoside compound k

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Compound K

[0028] 10 g of ginseng extract (red ginseng, white ginseng and the root hair and leaf of ginseng) was dissolved in 2 l of citrate buffer (pH 4.0). Thereto was added 10 g of naringinase (Sigma, St. Louis, Mo.), 10 g of pectinase (Novozyme, Copenhagen, Denmark) then the mixture was cultured in 38° C. of water bath for 48 hours. After enzymetic hydrolysis was completed, the reaction mixture was extracted with 2 l of ethylacetate and then evaporated under a vacuum condition to give 2.8 g of residue. For purification of compound K, the obtained product was subjected to silica gel column chromatography, eluted with chloroform- methanol (9:1) and then with chloroform-methanol (6:1), to give 0.28 g of pure compound K.

experimental example 1

Effect of Compound K on HAS2 Gene Expression in Human Epidernal Cell Line HaCaT

[0029]

[0030] Spontaneously immortalized human keratinocyte cell line, HaCaT, was provided by Dr. N.E. Fusenig (Deutsches Krebsforschungszentrum(DKFZ), Heidelberg, Germany) and human diploid fibroblast cell line, HDF, was provided by Dr. S.C. Park (Seoul National University, Seoul, Korea).

[0031] The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (HyClone), sodium bicarbonate (3.6g / l) and antibiotics of streptomycin (100 μg / ml) and penicillin (100 U / ml) (Life Technologies, Inc.) at 37° C. in a humidified atmosphere containing 5% CO2 and 95% air. The culture was changed with fresh medium every 3 days. On reaching the maximum cell-density, the cells were recultured at a 1:5 division ratio. 48 hours before treatment with compound K, the cells were seeded into a tissue culture flask at 1×105 cells per 75 cm2 and cultured in the medium with 10% fetal b...

experimental example 2

Effect of Compound K on Production of Hyaluronic Acid in Human Epidermal and Dermal Cell Lines

[0039] HaCaT and HDF cells were washed with PBS then fixed in a fixative with 2% paraformaldehyde(v / v) and 0.5% glutaraldehyde(v / v) at room temperature for 20 min. After fixation, the cells were washed three times for 2 min each with 0.1 M sodium phosphate buffer (pH7.4), then blocked in 1% bovine serum albumin (w / v) containing 0.1% Triton X-100 (v / v) in the same buffer at room temperature for 30 min. Hyaluronan staining was carried out with a specific probe of biotinylated hyaluronan binding protein (bHABP) (Seikagaku, Tokyo, Japan). The bHABP probe, diluted to 5 μg / ml in 3% bovine serum albumin (w / v), was added to the fixed cells and was incubated overnight at 4° C. Afterwashing, avidin-fluorescein isothocyanate (FITC) was added. Images were analyzed with fluorescent microscope and are shown in FIG. 3.

[0040]FIG. 3 shows the effect of compound K on the distribution of hyaluronic acid in ...

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Abstract

There are provided a promoter containing ginsenoside compound K for the production of hyaluronic acid, and more particularly, a new efficacy of 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol (compound K), a chief metabolite of ginseng saponin, to increase the expression of hyaluronic acid synthase gene in human cell and thereby to promote the production of hyaluronic acid, and an anti-aging agent containing the promoter for the production of hyaluronic acid as an effective ingredient.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a promoter containing ginsenoside compound K for the production of hyaluronic acid. More particularly, the present invention provides a new efficacy of 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol (called “compound K”), a chief metabolite of ginseng saponin, to increase the expression of the hyaluronic acid synthase (HAS) gene in human cells and thereby to promote the production of hyaluronic acid (HA), and provides a promoter containing compound K for the production of hyaluronic acid. BACKGROUND OF THE INVENTION [0002] Hyaluronic acid is a nonsulfated glycosaminoglycan, linear polysaccharide with a very large molecular weight of 200,000 to 400,000 composed of repeating glucuronic acid and N-acetylglucosamine residues. Hyaluronic acid is a major structural component of the extracellular matrix and involved in water retention, maintenance of the extracellular space and storage and diffusion of cell growth factors and nu...

Claims

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Application Information

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IPC IPC(8): A61K31/704C07J17/00
CPCA61K8/735A61K31/704A61Q19/08A61P17/00A61P19/02A61P43/00C07J17/00
Inventor KIM, SU-JONGKANG, BYUNG-YOUNGCHO, SI-YOUNGCHANG, HUI-KYOUNGSUNG, DAE-SEOKYEOM, MYEONG-HOONWOE, KWANG-SIKKIM, DUCK-HEEKIM, HAN-KONSIM, YOUNG-CHULKANG, HAK-HEELEE, YONG-SUNG
Owner AMOREPACIFIC CORP
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