6 results about "Hyaluronan binding" patented technology

The invention discloses a preparation method of anti-tumor hyaluronic acid-naringenin self-assembled nanoparticles, and belongs to the field of medicines. According to the method, carboxyl on hyaluronic acid and hydroxyl on naringenin are combined to form an ester group, and the hyaluronic acid-naringenin polymer is obtained. Hydrophilic hyaluronic acid is combined with hydrophobic naringenin to form a hydrophilic and hydrophobic polymer, then under the action of ultrasonic waves in an aqueous solution, the hydrophobic end repels water molecules and is mutually gathered, and the hydrophilic end wraps the periphery of the hydrophobic end and is in contact with water, so that the structure that the core is hydrophobic naringenin and the core is hydrophobic naringenin is formed. The outer core is a nanoparticle of hydrophilic hyaluronic acid. The particle size of the nanoparticles is 140-200nm, the nanoparticles are stable and uniform, in-vitro release experiment results show that the nanoparticles can be slowly released, and cell experiments show that the preparation can obviously improve the toxicity of naringenin to tumor cells, which shows that the nanoparticles have good anti-tumor activity.

The present disclosure is directed to a method that involves selecting a subject having a primary tumor and obtaining, from the selected subject, a sample containing exosomes derived from primary tumor cells. The exosomes are isolated from the sample and expression levels of cell migration-inducing and hyaluronan-binding protein (CEMIP) are detected in the isolated exosomes.

The invention provides a composition containing keratinase. The composition is prepared from the following components: 5000 to 20000 U / g of keratinase, 2 to 10 wt% of calcium thioglycolate, 0.5 to 5 wt% of folium cortex eucommiae extract, 0.3 to 2 wt% of eucalyptus multibracteata essential oil, more than or equal to 70% of cineole and 0.05 to 1 wt% of hyaluronic acid binding peptide HABP. The composition is used for preparing the painless depilatory paste. The keratinase is innovatively compounded with the calcium thioglycolate and other components, so that the skin irritation is remarkably reduced while efficient hair removal is ensured, and the keratinase hair removal cream has the multiple advantages of long-acting hair inhibition, immediate repair, use experience improvement and the like.

The present invention relates to new hyaluronic acid binding proteins. The invention refers to new engineered fusion proteins for use in treating disorders of the eye or the joints. The fusion proteins comprise a subunit that specifically binds to hyaluronic acid. In addition to the hyaluronic acid binding protein, the new fusion proteins comprise a protein therapeutically effective in diseases of the eye or of joints. The fusion proteins are particularly well-suited for medical applications that require extended half-life of eye specific therapeutic proteins.

This invention belongs to the field of contact lens technology, and provides a moisturizing protein-based contact lens and its preparation method. The preparation method includes the following steps: mixing a silk fibroin solution and a hyaluronic acid-binding protein solution to obtain a protein mixture solution; mixing a hydrophilic modification component, an oxygen-permeable modification component, and water to obtain a composite modifier; mixing the protein mixture solution, the composite modifier, and a crosslinking agent to obtain a pre-crosslinked mixture; molding the pre-crosslinked mixture and performing gradient crosslinking, followed by sequential soaking in buffer solution, rinsing, and sterilization to obtain the moisturizing protein-based contact lens. The contact lens of this invention uses protein as the main component, does not rely on silicon-based substances or harmful crosslinking agents, and possesses high biocompatibility, high oxygen permeability, suitable mechanical properties, and controllable degradation.

The invention discloses polypeptide type micromolecule glue and application thereof in preparation of cosmetics with a skin anti-aging effect. The polypeptide type small molecule glue comprises a hyaluronic acid binding peptide fragment and a proteoglycan binding peptide fragment. The polypeptide type small molecule glue provided by the invention is polypeptide with biological activity, the polypeptide can be combined with hyaluronic acid and proteoglycan after entering cells, the function of connecting the hyaluronic acid and the proteoglycan to protein 1 (HAPLN1) can be replaced, the content of collagen which is gradually reduced along with skin aging can be recovered to a certain extent, and the collagen type small molecule glue has the advantages of being good in biocompatibility, good in stability and good in stability. The composition may become a promising innovative strategy for preventing or repairing the aged skin.