The invention relates to the technical field of breeding, and discloses a young chicken colibacillosis prevention method. The method comprises the steps of brooding room treatment, young chicken eggshell breaking treatment, brooding management and young chicken treatment; before the young chicken enters the brooding room, conducting two times of sterilization and disinfection on the brooding room, using no chemical disinfection agent, which is safe and environmentally friendly and produces no drug resistance; after the young chicken enters the brooding room, regularly conducting sterilization treatments by using the Chinese medicine solutions, killing the escherichia coli, and also increasing the anti-disease ability of the young chicken; after the young chicken breaks the eggshell, immediately spraying the special agent for eggshell breaking, ensuring the required humidity of the young chicken, killing etiology microbes, increasing the adaptability to the outside environment of the young chicken, and increasing the anti disease ability; after the young chicken enters the brooding room, in advance feeding the young chicken on water agent and feeding material with Chinese medicine content, increasing the functionality of stomach and intestines, training the digestion ability of stomach and intestines, increasing the resistibility to the escherichia coli, lowering the illness rate to 2-3%, and lowering the breeding cost to 13.7%.