The invention relates to a keep-alive system and a keep-alive method, and belongs to the field of keep-alive transportation. A keep-alive transportation vehicle for circulation transportation is characterized in that the transportation vehicle is divided into two relatively independent space regions including a keep-alive object loading region and a keep-alive equipment region, electric power supply equipment, a heat and cold supply system, an atomization spraying device, aeration oxygenation equipment, purification sterilization equipment, a compartment environment monitoring system and an intelligent control system are respectively arranged in the keep-alive equipment region, the intelligent control system controls and maintains the operation work of all systems and equipment of the keep-alive equipment region, the heat and cold supply system can realize the refrigeration mode of cold air preparation or cold water preparation, or can convert the refrigeration mode into a heat pump heating mode of hot air preparation or hot water preparation, the intelligent control system can control the operation of all equipment or systems in the keep-alive transportation vehicle according to keep-alive objects or keep-alive process requirements, and the independent work of any one keep-alive mode of three keep-alive modes is realized. Through the system and the method, the keep-alive transportation adaptation capability can be greatly improved.