The invention provides a
virus vector capable of realizing
polygene editing of plants as well as a construction method and application of the
virus vector, and relates to the technical field of
gene editing of the plants. According to the
virus vector, tRNA and sgRNA are sequentially linked to form unit sequences, and the unit sequences are linked with vectors pCE2; a target sequence of a
target gene is inserted between the tRNA and sgRNA of each unit sequence by virtue of a ''golden-gate''
cloning method, the multiple unit sequences are linked and arrayed in series to form a series-wound sequence, the series-wound sequence is inserted into a TRV2 virus vector, after plants capable of overexpressing Cas9 proteins are infected by TRV, and a tRNA-sgRNA expression sequence unit assembled withdifferent unit sequences is transcribed, thereby finishing a
polygene editing process, so that editing of multiple genes or combined editing of different genes according to targets can be realized. According to the construction method, the expression quantity of mRNA capable of being translated into
normal protein sequences in the plants is lower, a
gene silencing
phenotype is relatively stable,and meanwhile, multiple genes can be precisely knocked out in a targeting manner.