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Virus vector capable of realizing polygene editing of plants as well as construction method and application of virus vector

ActiveCN111334527APrecise Targeted KnockoutLow mRNA expressionMicrobiological testing/measurementFermentationBiotechnologyGolden Gate Cloning
The invention provides a virus vector capable of realizing polygene editing of plants as well as a construction method and application of the virus vector, and relates to the technical field of gene editing of the plants. According to the virus vector, tRNA and sgRNA are sequentially linked to form unit sequences, and the unit sequences are linked with vectors pCE2; a target sequence of a target gene is inserted between the tRNA and sgRNA of each unit sequence by virtue of a ''golden-gate'' cloning method, the multiple unit sequences are linked and arrayed in series to form a series-wound sequence, the series-wound sequence is inserted into a TRV2 virus vector, after plants capable of overexpressing Cas9 proteins are infected by TRV, and a tRNA-sgRNA expression sequence unit assembled withdifferent unit sequences is transcribed, thereby finishing a polygene editing process, so that editing of multiple genes or combined editing of different genes according to targets can be realized. According to the construction method, the expression quantity of mRNA capable of being translated into normal protein sequences in the plants is lower, a gene silencing phenotype is relatively stable,and meanwhile, multiple genes can be precisely knocked out in a targeting manner.
Owner:JIANGSU UNIV OF SCI & TECH

Information management system and method based on big data

The invention provides an information management method based on big data, and the method comprises the following steps: obtaining an input data stream, carrying out the association of image attribute data and text attribute data according to a data generation time sequence, and obtaining a plurality of groups of associated data streams; taking the current associated data stream as the input of a deep learning model; when the output result meets the first performance condition, starting a first video memory allocation process to allocate a first video memory space, and storing the next associated data stream in the first video memory space; when the video memory occupancy rate of the first video memory space exceeds a preset video memory capacity control threshold value, starting a second video memory allocation process to allocate a second video memory space, and storing the next associated data stream into the second video memory space; and judging whether the total capacity of the allocated video memory space exceeds a system control value or not, and if so, transferring the target associated data of the allocated video memory space to a memory component. The invention further discloses a system for implementing the method and a computer readable storage medium.
Owner:苏州优鲜信网络生活服务科技有限公司
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